Reviewed, UniProtKB/Swiss-Prot Q2IU52 (DNAE2_RHOP2)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	RPB_3563

Organism

Rhodopseudomonas palustris (strain HaA2) [Complete proteome] [HAMAP]

Taxonomic identifier

316058 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	1165 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1165	1165	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000070595

Sequences

	Sequence		Length	Mass (Da)
	Q2IU52-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 8CE6E61C64440D16	FASTA	1,165	129,923
	10 20 30 40 50 60 MNAPRYAEIG VTTNFSFLEG GSHPQDYVHQ ASRLGLDAIG IADRNTLAGV VRAYSELDNE 70 80 90 100 110 120 ELAYKPKLLI GARLCFVDGT PDVLAYPTDR AAYGRLCRLL SAGKLRAGKG ECHLTFADLE 130 140 150 160 170 180 AFVSSNAARP FPSSWPGLTR PSTSLQPRVL QDVDARDKPG HDGGKREIGR SQILFVLMPP 190 200 210 220 230 240 YRFQAKAITA ALERLTALNS GNVWLALAPY YRGDDKRRLA RLRRIAAAAD VPGIATNDVL 250 260 270 280 290 300 YHHPSRRALQ DVLTCVRDKT TIDKAGRRLE GNAERHLKPA AEMARLFRAD LDAVAETLRF 310 320 330 340 350 360 ADRISFTLDE LKYHYPDEPV PPGKTAQQHL EDLTREGIAT YFPNGISDRL RATIAKELTL 370 380 390 400 410 420 IAKRDYAHYF LTVHDIVRYA RSQNILCQGR GSAANSAVCY VLGITCVDPT EIDLLFERFV 430 440 450 460 470 480 SEERDEPPDI DVDFEHSRRE EVMQYIYRRY GRHRAAIVST VIHYRPRSAI RDVGKALGLS 490 500 510 520 530 540 EDVTAALADT VWGSWGKGLS EMQVRQAGLD PTNPMIGRAV ELATELIGFP RHLSQHVGGY 550 560 570 580 590 600 VLTQDRLDSY VPIGNAAMAD RTFIEWDKDD IDAVKMMKVD VLALGMLTCI RKGFDLIAQH 610 620 630 640 650 660 KGVRFQLSDI KSEDDNNVYQ MLQRGESIGV FQVESRAQMN MLPRLKPRCF YDLVIEVAIV 670 680 690 700 710 720 RPGPIQGDMV HPYLRRRNGQ EPVVYPSPSG EAGDKNELQQ ILGKTLGVPL FQEQAMRIAI 730 740 750 760 770 780 EAAHFTPDEA NQLRRAMATF RNVGTIGKFE SKMIGNLMAR GYDATFAKNC FEQIKGFGSY 790 800 810 820 830 840 GFPESHAASF AKLVYVSAWM KCEHPDAFCC ALLNSQPMGF YAPAQIVGDA RANKVEVRPV 850 860 870 880 890 900 DVSFSDGQCT LEERCGAYHA VRLGFRQIDG FRWADPDEER VRLEAGLPPS DDWAARIVAA 910 920 930 940 950 960 RARGPFGSLE QFARITALPK RALILLADAD AFRSLGLDRR AALWAVRRLP DDVPLPLFEA 970 980 990 1000 1010 1020 ASAREQQDEQ AAPLPQMPMA EHVVADYQTV RLSLKGHPLE FLRAVFATER VVTCREVSET 1030 1040 1050 1060 1070 1080 RRNGRRVRCA GVVLVRQRPG SASGVIFMTI EDETGIANIV VWPSVMEKFR KEVMGARLIL 1090 1100 1110 1120 1130 1140 VEGKIQASPE GVVHLVAERL VDRSSEMGRL SEGLARPPLP TGADLYEPLT YEPLNGDRRD 1150 1160 NPDAPAQRLR HPRDVRILPP SRDFH

References

[1]

"Complete sequence of Rhodopseudomonas palustris HaA2." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A. , Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000250 Genomic DNA. Translation: ABD08258.1.
RefSeq	YP_487169.1.
3D structure databases
HSSP	HSSP built from PDB template 1X56 based on UniProtKB O57980.
SMR	Q2IU52. Positions 1-949.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IU52.
Genome annotation databases
GeneID	3911365.
GenomeReviews	Gene locus RPB_3563 in contig CP000250_GR.
KEGG	rpb:RPB_3563.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	MGFYSPD.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_RHOP2

Accession

Primary (citable) accession number: Q2IU52

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)