Q2ISZ6 (ACSF_RHOP2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
Short name=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase
EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	RPB_3971

Organism

Rhodopseudomonas palustris (strain HaA2) [Complete proteome] [HAMAP]

Taxonomic identifier

316058 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Rhodopseudomonas

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity. HAMAP MF_01840
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840
Cofactor	Iron By similarity. HAMAP MF_01840
Pathway	Porphyrin biosynthesis; bacteriochlorophyll biosynthesis (light-independent). HAMAP MF_01840
Sequence similarities	Belongs to the AcsF family.

Ontologies

Keywords
Biological process	Bacteriochlorophyll biosynthesis Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	bacteriochlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840		PRO_1000070550

Sequences

	Sequence		Length	Mass (Da)
	Q2ISZ6 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 09DC0949FD6B91F5	FASTA	365	42,415
	10 20 30 40 50 60 MIPMEGGAQS ALRSRPAIKG SVESLNIAKE DTILTPRFYT TDYAAMDKLD VSLVRSEWNV 70 80 90 100 110 120 MMNEMRADYN KSHFKKNDEF LESDLDKLPP ALRAEFKDFL VSSLTAEFSG CVLYAEIKKR 130 140 150 160 170 180 IKNPEIRELF GLLSRDEARH AGFINEILKD HGIGVDLSFL TKVKKYTYFR PKFIFYATYL 190 200 210 220 230 240 SEKIGYARYI TIYRQMERHP ERRFHPIFKW FERWCNDEFR HGEAFALLMR ADPSLLSGVN 250 260 270 280 290 300 KLWIRFFLLA VFATMYVRDH MRPAFYEALG MDAAEYGMQV FRITTEISKQ VFPVTINLDD 310 320 330 340 350 360 PRFLAGLERL RVASEKLADC RSQGFVGKLK RPFYVASAAL AFGRLFLLPA KRNELPRVIG LRPAW

References

[1]

"Complete sequence of Rhodopseudomonas palustris HaA2." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Pelletier D.A. , Kyrpides N., Anderson I., Oda Y., Harwood C.S., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HaA2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000250 Genomic DNA. Translation: ABD08664.1.
RefSeq	YP_487575.1. NC_007778.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2ISZ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3911778.
GenomeReviews	Gene locus RPB_3971 in contig CP000250_GR.
KEGG	rpb:RPB_3971.
PATRIC	23302779. VBIRhoPal125544_4158.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG10633.
HOGENOM	HBG284843.
OMA	HGDFFDA.
ProtClustDB	PRK13654.
Enzyme and pathway databases
BioCyc	RPAL316058:RPB_3971-MONOMER.
Family and domain databases
HAMAP	MF_01840. AcsF. [Tree]
InterPro	IPR008434. AcsF. IPR012347. Ferritin-rel. IPR009078. Ferritin/RR-like. IPR003251. Rubrerythrin. [Graphical view]
Gene3D	G3DSA:1.20.1260.10. Ferritin_rel. 1 hit.
KO	K04035.
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_RHOP2

Accession

Primary (citable) accession number: Q2ISZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program