Reviewed, UniProtKB/Swiss-Prot Q2IQI6 (PYRD_ANADE)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	Adeh_1294

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity. HAMAP MF_00225
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity. HAMAP MF_00225
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000024149
Sites
	Active site	178	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IQI6-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: B750C3601ADADEC6	FASTA	364	38,378
	10 20 30 40 50 60 MLWPALRSVL FQLDPERVHH LAHWALHRVP PALARARRPA PVPALAVRCM GLDFDGPMGL 70 80 90 100 110 120 AAGFDKGDVA LPGLFGLGFS HVEIGTITPR PQPGNDRPRL FRLPEHRALL NRMGFNNEGM 130 140 150 160 170 180 EACARRLAAL PAAARLGPVG INVGKNKATP NEDAASDYLA CIERLHPYAD YLVVNISSPN 190 200 210 220 230 240 TPGLRQLQER EALDRLLRAC VRHLAERAPG KPLLVKLAPD LSPEALDEAV DVAIAAGAAG 250 260 270 280 290 300 IVATNTTLSR AGVERHPRAA EAGGLSGAPL ERLATEVVRR CYARAAGRVP IVGVGGVMDA 310 320 330 340 350 360 EGAYAKIRAG ATLVQAYTGL IYGGPGFVGR VNAGLARLLE RDGFSTLSDA VGADHRQGGG KAAG

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC81068.1.
RefSeq	YP_464505.1.
3D structure databases
SMR	Q2IQI6. Positions 1-335.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IQI6.
Genome annotation databases
GeneID	3888688.
GenomeReviews	Gene locus Adeh_1294 in contig CP000251_GR.
KEGG	ade:Adeh_1294.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0167.
HOGENOM	HBG351027.
OMA	AALNRMG.
PhylomeDB	Q2IQI6.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1294-MONOMER.
Family and domain databases
HAMAP	MF_00225. DHO_dh_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_ANADE

Accession

Primary (citable) accession number: Q2IQI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)