Reviewed, UniProtKB/Swiss-Prot Q2IQG8 (ISPDF_ANADE)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme ispD/ispF
Including the following 2 domains:
    1- Recommended name:
            2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
              EC=2.7.7.60
        Alternative name(s):
            4-diphosphocytidyl-2C-methyl-D-erythritol synthase
            MEP cytidylyltransferase
              Short name=MCT
    2- Recommended name:
            2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
                Short name=MECPS
                Short name=MECDP-synthase
              EC=4.6.1.12

Gene names

Name:	ispDF
Ordered Locus Names:	Adeh_1272

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (ispD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (ispF) By similarity.
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_01520 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_01520
Cofactor	Divalent metal cations By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_01520 Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
Sequence similarities	In the N-terminal section; belongs to the ispD family. In the C-terminal section; belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	Bifunctional enzyme ispD/ispF HAMAP MF_01520		PRO_0000296738
Regions
	Region	1 – 229	229	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_01520
	Region	230 – 386	157	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_01520
Sites
	Metal binding	236	1	Divalent metal cation By similarity
	Metal binding	238	1	Divalent metal cation By similarity
	Metal binding	269	1	Divalent metal cation By similarity
	Site	19	1	Transition state stabilizer By similarity
	Site	24	1	Transition state stabilizer By similarity
	Site	155	1	Positions MEP for the nucleophilic attack By similarity
	Site	211	1	Positions MEP for the nucleophilic attack By similarity
	Site	261	1	Transition state stabilizer By similarity
	Site	360	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IQG8-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 6FEF0790B3FD25E4	FASTA	386	39,134
	10 20 30 40 50 60 MIRGERVIGI LAAGGSGQRA GVAKQWLVLG GESVLRRSAR VLAACDAVDG LVVVVPPGDE 70 80 90 100 110 120 ARGEAELAGL GKPVRAVAGG PARADSVRNG LAAADGAVVL VHDAARPFAS AALAGRVAEA 130 140 150 160 170 180 AARDGAALAA LPATDTVKRA EAGAEVPRVL ETLDRRTVWL AQTPQGFRRA VLEQAYAAAG 190 200 210 220 230 240 PSASAATDEC ALVEAAGAPV TLVPGEPGNF KITGPDDVRR ARALLEAPVA TGVGYDTHRF 250 260 270 280 290 300 APGRRLVLGG VEFEGDGLLG HSDADVCAHA IGDAILGAAG LGDLGRHFPD TDPRWKGVSS 310 320 330 340 350 360 LALLREIAAK AAERGWRVGN CDVTLAAKRP KIAPRAEEMR ARLAGALGIS PAQVNVKATT 370 380 GEGMGFVGRE EGVAAHAIAL LVRAAG

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC81046.1.
RefSeq	YP_464483.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3888705.
GenomeReviews	Gene locus Adeh_1272 in contig CP000251_GR.
KEGG	ade:Adeh_1272.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IQG8.
OMA	Q2IQG8. IVLIHDA.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1272-MON.
Family and domain databases
HAMAP	MF_01520. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. IPR003526. MECDP_synthase_core. [Graphical view]
Pfam	PF01128. IspD. 1 hit. PF02542. YgbB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. ispD. 1 hit. TIGR00151. ispF. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. PS01350. ISPF. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPDF_ANADE

Accession

Primary (citable) accession number: Q2IQG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)