Reviewed, UniProtKB/Swiss-Prot Q2IQG7 (SYC_ANADE)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	Adeh_1273

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_0000240887
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	272 – 276	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	210	1	Zinc By similarity
	Metal binding	235	1	Zinc By similarity
	Metal binding	239	1	Zinc By similarity
	Binding site	275	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IQG7-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 190A70B0FFE8E0B1	FASTA	481	53,174
	10 20 30 40 50 60 MSIQVHDTLT AQKRELVPLE PGKLRLYVCG PTVYDYSHLG HARCYVVWDV VVRHLRARGL 70 80 90 100 110 120 EVRFVRNFTD VDDKIIQRAN ERGEDPIALA SRFADAFHED MDALGNLRPD VEPRVSGHIP 130 140 150 160 170 180 EIVALIARLV ERGFAYAPGN GDVYYAVRKF PEYGRLSKRN LDDLIAGARV EPGEAKRDPL 190 200 210 220 230 240 DFALWKAAKP GEPAWDSPWG KGRPGWHIEC SAMTQKHLGA PIDLHAGGKD LVFPHHTNEI 250 260 270 280 290 300 AQSVAATSDG LHAEDFARYW MHNGFVQIDD EKMSKSLGNF FTIRDVLARF DGEALRFFLL 310 320 330 340 350 360 GTHYRRDFNF SDQVLAEAER RLSALYETVE KAERLGAGVE PGAEPAFVER ARAALDDDFN 370 380 390 400 410 420 TPQVLGIVAE AFTEANALAD RKGKKSPEEK ARLAAFARGA RAVGAVLGIL GRPPAQALSA 430 440 450 460 470 480 IRDRAAARRG IDGSEVERLI AERAAARAAK DFARSDAIRD GLLARGVVLM DGPQGTTWKV E

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC81047.1.
RefSeq	YP_464484.1.
3D structure databases
SMR	Q2IQG7. Positions 4-416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IQG7.
Genome annotation databases
GeneID	3888706.
GenomeReviews	Gene locus Adeh_1273 in contig CP000251_GR.
KEGG	ade:Adeh_1273.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HBG327651.
OMA	VLWKAAK.
PhylomeDB	Q2IQG7.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1273-MONOMER.
Family and domain databases
HAMAP	MF_00041_B. Cys_tRNA_synth_B. [Tree]
InterPro	IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ANADE

Accession

Primary (citable) accession number: Q2IQG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)