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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Anaeromyxobacter dehalogenans (strain 2CP-C)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi495 – 4951MagnesiumUniRule annotation
Metal bindingi501 – 5011MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciADEH290397:GI2Z-1116-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Adeh_1107
OrganismiAnaeromyxobacter dehalogenans (strain 2CP-C)
Taxonomic identifieri290397 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeAnaeromyxobacteraceaeAnaeromyxobacter
ProteomesiUP000001935: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Polyribonucleotide nucleotidyltransferasePRO_0000329498Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290397.Adeh_1107.

Structurei

3D structure databases

ProteinModelPortaliQ2IQ01.
SMRiQ2IQ01. Positions 627-701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini562 – 62160KHUniRule annotationAdd
BLAST
Domaini631 – 69969S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2IQ01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPIQKTATV GGKEVLLETG KVAKQAHGSV WVRLGDSIVL VTAVSAAEKK
60 70 80 90 100
EGIDFFPLTV DYQEKLFAAG RVPGSFFRRE GRPTEKETLT SRLVDRSCRP
110 120 130 140 150
LFAEGYSNET QVIATVISFD QENDTDVLAL TGASAALHIS DIPFGGPIAG
160 170 180 190 200
VRVARVGGQL VANPTLAQRA DADLDVVMAA SRDAIVMVEG GAQEVSEAVM
210 220 230 240 250
IEALLFGQAA VQPLLDAQDA LRAATGNKPR RAFDPPKNDV ELRAKVKALT
260 270 280 290 300
WEKVKEAYGR DEKHDRYGRL SEIKKELLQA LKEEAAGDAA KLATIALREK
310 320 330 340 350
EIKGYYEDVK YDYMRKMITD ERRRIGGRGM ADIRKITCEV GLLPRVHGSS
360 370 380 390 400
LFTRGETQAL VATTLGTAED EQRVEMLTGM VFKKFMLHYN FPPFSVGEVK
410 420 430 440 450
FLRSPGRREI GHGALAERAL RAVMPPEDQF PYTVRVVSDI MESNGSSSMA
460 470 480 490 500
SVCGGCLSLM DAGVPIKAPV AGIAMGLIKE GEKIAILSDI LGDEDHLGDM
510 520 530 540 550
DFKVCGTAAG ITSIQMDIKI GGVTREILEQ ALSQAAEGRK HILGEMAKAL
560 570 580 590 600
SAPRGSISAY APRITTIKIR PERIKDIIGP GGKTIKDITA RTGTSINIED
610 620 630 640 650
DGSVSIASPN QDKVEEAIKM IRGLTQEAEV GRIYMGTVRK IAEFGAFVEI
660 670 680 690 700
FPGTDGLIHI SELSDKRVKS VSDVLSEGEE VMVKVISVDR SGKIRLSRKE
710 720
ALADSAKKSE GTEPPKGEPA K
Length:721
Mass (Da):77,920
Last modified:April 29, 2008 - v2
Checksum:i01A55B2271539260
GO

Sequence cautioni

The sequence ABC80882.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000251 Genomic DNA. Translation: ABC80882.1. Different initiation.
RefSeqiYP_464319.1. NC_007760.1.

Genome annotation databases

EnsemblBacteriaiABC80882; ABC80882; Adeh_1107.
GeneIDi3888643.
KEGGiade:Adeh_1107.
PATRICi20918297. VBIAnaDeh31384_1146.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000251 Genomic DNA. Translation: ABC80882.1. Different initiation.
RefSeqiYP_464319.1. NC_007760.1.

3D structure databases

ProteinModelPortaliQ2IQ01.
SMRiQ2IQ01. Positions 627-701.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290397.Adeh_1107.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABC80882; ABC80882; Adeh_1107.
GeneIDi3888643.
KEGGiade:Adeh_1107.
PATRICi20918297. VBIAnaDeh31384_1146.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciADEH290397:GI2Z-1116-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 2CP-C.

Entry informationi

Entry nameiPNP_ANADE
AccessioniPrimary (citable) accession number: Q2IQ01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: January 7, 2015
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.