Reviewed, UniProtKB/Swiss-Prot Q2IQ01 (PNP_ANADE)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	Adeh_1107

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000329498
Regions
	Domain	562 – 621	60	KH
	Domain	631 – 699	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q2IQ01-1 [UniParc]. Last modified April 29, 2008. Version 2. Checksum: 01A55B2271539260	FASTA	721	77,920
	10 20 30 40 50 60 MTPIQKTATV GGKEVLLETG KVAKQAHGSV WVRLGDSIVL VTAVSAAEKK EGIDFFPLTV 70 80 90 100 110 120 DYQEKLFAAG RVPGSFFRRE GRPTEKETLT SRLVDRSCRP LFAEGYSNET QVIATVISFD 130 140 150 160 170 180 QENDTDVLAL TGASAALHIS DIPFGGPIAG VRVARVGGQL VANPTLAQRA DADLDVVMAA 190 200 210 220 230 240 SRDAIVMVEG GAQEVSEAVM IEALLFGQAA VQPLLDAQDA LRAATGNKPR RAFDPPKNDV 250 260 270 280 290 300 ELRAKVKALT WEKVKEAYGR DEKHDRYGRL SEIKKELLQA LKEEAAGDAA KLATIALREK 310 320 330 340 350 360 EIKGYYEDVK YDYMRKMITD ERRRIGGRGM ADIRKITCEV GLLPRVHGSS LFTRGETQAL 370 380 390 400 410 420 VATTLGTAED EQRVEMLTGM VFKKFMLHYN FPPFSVGEVK FLRSPGRREI GHGALAERAL 430 440 450 460 470 480 RAVMPPEDQF PYTVRVVSDI MESNGSSSMA SVCGGCLSLM DAGVPIKAPV AGIAMGLIKE 490 500 510 520 530 540 GEKIAILSDI LGDEDHLGDM DFKVCGTAAG ITSIQMDIKI GGVTREILEQ ALSQAAEGRK 550 560 570 580 590 600 HILGEMAKAL SAPRGSISAY APRITTIKIR PERIKDIIGP GGKTIKDITA RTGTSINIED 610 620 630 640 650 660 DGSVSIASPN QDKVEEAIKM IRGLTQEAEV GRIYMGTVRK IAEFGAFVEI FPGTDGLIHI 670 680 690 700 710 720 SELSDKRVKS VSDVLSEGEE VMVKVISVDR SGKIRLSRKE ALADSAKKSE GTEPPKGEPA K

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC80882.1. Different initiation.
RefSeq	YP_464319.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3888643.
GenomeReviews	Gene locus Adeh_1107 in contig CP000251_GR.
KEGG	ade:Adeh_1107.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IQ01.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1107-MON.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_ANADE

Accession

Primary (citable) accession number: Q2IQ01

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 29, 2008
Last modified:	June 16, 2009
This is version 23 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)