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Q2IQ01 (PNP_ANADE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8
Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase
Gene names
Name:pnp
Ordered Locus Names:Adeh_1107
OrganismAnaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]
Taxonomic identifier290397 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length721 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP MF_01595

Catalytic activity

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595

Subunit structure

Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_01595.

Sequence similarities

Belongs to the polyribonucleotide nucleotidyltransferase family.

Contains 1 KH domain.

Contains 1 S1 motif domain.

Sequence caution

The sequence ABC80882.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandRNA-binding
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processRNA processing

Inferred from electronic annotation. Source: InterPro

mRNA catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3'-5'-exoribonuclease activity

Inferred from electronic annotation. Source: InterPro

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

polyribonucleotide nucleotidyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 721721Polyribonucleotide nucleotidyltransferase HAMAP MF_01595
PRO_0000329498

Regions

Domain562 – 62160KH
Domain631 – 69969S1 motif

Sequences

Sequence LengthMass (Da)Tools
Q2IQ01 [UniParc].

Last modified April 29, 2008. Version 2.
Checksum: 01A55B2271539260

FASTA72177,920
        10         20         30         40         50         60 
MTPIQKTATV GGKEVLLETG KVAKQAHGSV WVRLGDSIVL VTAVSAAEKK EGIDFFPLTV 

        70         80         90        100        110        120 
DYQEKLFAAG RVPGSFFRRE GRPTEKETLT SRLVDRSCRP LFAEGYSNET QVIATVISFD 

       130        140        150        160        170        180 
QENDTDVLAL TGASAALHIS DIPFGGPIAG VRVARVGGQL VANPTLAQRA DADLDVVMAA 

       190        200        210        220        230        240 
SRDAIVMVEG GAQEVSEAVM IEALLFGQAA VQPLLDAQDA LRAATGNKPR RAFDPPKNDV 

       250        260        270        280        290        300 
ELRAKVKALT WEKVKEAYGR DEKHDRYGRL SEIKKELLQA LKEEAAGDAA KLATIALREK 

       310        320        330        340        350        360 
EIKGYYEDVK YDYMRKMITD ERRRIGGRGM ADIRKITCEV GLLPRVHGSS LFTRGETQAL 

       370        380        390        400        410        420 
VATTLGTAED EQRVEMLTGM VFKKFMLHYN FPPFSVGEVK FLRSPGRREI GHGALAERAL 

       430        440        450        460        470        480 
RAVMPPEDQF PYTVRVVSDI MESNGSSSMA SVCGGCLSLM DAGVPIKAPV AGIAMGLIKE 

       490        500        510        520        530        540 
GEKIAILSDI LGDEDHLGDM DFKVCGTAAG ITSIQMDIKI GGVTREILEQ ALSQAAEGRK 

       550        560        570        580        590        600 
HILGEMAKAL SAPRGSISAY APRITTIKIR PERIKDIIGP GGKTIKDITA RTGTSINIED 

       610        620        630        640        650        660 
DGSVSIASPN QDKVEEAIKM IRGLTQEAEV GRIYMGTVRK IAEFGAFVEI FPGTDGLIHI 

       670        680        690        700        710        720 
SELSDKRVKS VSDVLSEGEE VMVKVISVDR SGKIRLSRKE ALADSAKKSE GTEPPKGEPA 


K

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References

[1]"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2CP-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000251 Genomic DNA. Translation: ABC80882.1. Different initiation.
RefSeqYP_464319.1. NC_007760.1.

3D structure databases

ProteinModelPortalQ2IQ01.
SMRQ2IQ01. Positions 627-701.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2IQ01.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3888643.
GenomeReviewsGene locus Adeh_1107 in contig CP000251_GR.
KEGGade:Adeh_1107.
PATRIC20918297. VBIAnaDeh31384_1146.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1185.
HOGENOMHBG382411.
ProtClustDBPRK11824.

Enzyme and pathway databases

BioCycADEH290397:ADEH_1107-MONOMER.

Family and domain databases

HAMAPMF_01595. PNPase.
[Tree]
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH.
IPR009019. KH_prok-type.
IPR004088. KH_type_1.
IPR018111. KH_type_1_subgr.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR012162. PNPase.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
KOK00962.
PANTHERPTHR11252. PNPase. 1 hit.
PfamPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFPIRSF005499. PNPase. 1 hit.
SMARTSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF46915. 3_ExoRNase. 1 hit.
SSF55666. 3_ExoRNase. 2 hits.
SSF54814. KH_prok. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMsTIGR03591. Polynuc_phos. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNP_ANADE
AccessionPrimary (citable) accession number: Q2IQ01
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: January 25, 2012
This is version 40 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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