Reviewed, UniProtKB/Swiss-Prot Q2IPT7 (SYY_ANADE)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	Adeh_1044

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity. HAMAP MF_02006
Subcellular location	Cytoplasm By similarity HAMAP MF_02006.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	Tyrosyl-tRNA synthetase HAMAP MF_02006		PRO_0000234664
Regions
	Domain	353 – 419	67	S4 RNA-binding
	Motif	38 – 47	10	"HIGH" region HAMAP MF_02006
	Motif	227 – 231	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	33	1	Tyrosine By similarity
	Binding site	167	1	Tyrosine By similarity
	Binding site	171	1	Tyrosine By similarity
	Binding site	230	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IPT7-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: BB93A25BBA1431C1	FASTA	420	45,350
	10 20 30 40 50 60 MQNLLEALVP RSLVHDQTPG LQARLAQGPI TGYVGFDPTA DSLHVGHLLA VMSLAWLQRC 70 80 90 100 110 120 GGTPIIVVGG GTGMVGDPSG KRSERPVLSV EEIDRNVAAI RAQLERFVSF EGQNAARVRN 130 140 150 160 170 180 NADWLRSIGL MEFLRDVGKH FTVNYMLAKD SVKGRMESGI SFTEFSYQLI QAYDFWHLFH 190 200 210 220 230 240 AERCELQMGG SDQWGNITAG AELVSRKDGA SVHGLTFPLL TTASGTKFGK TEGGAVWLDP 250 260 270 280 290 300 ARTSPYKFFQ FWLNTDDRDV ERLLKFFTFL SLDEIAALLA EQARDPGKRP AQRRLAEDVT 310 320 330 340 350 360 ARVHGPDVTR SVIEASRILF GGTDLRAAGV DVLDVLAGEI PSATVTGDEL AALTVADLLV 370 380 390 400 410 420 KVGLAASKGE VRRGVAGRGF SLNGAVLESG DAKVAAGELL AGGYALLQKG KRNYALVKVR

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC80819.1.
RefSeq	YP_464256.1.
3D structure databases
SMR	Q2IPT7. Positions 3-309, 4-419.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IPT7.
Genome annotation databases
GeneID	3888470.
GenomeReviews	Gene locus Adeh_1044 in contig CP000251_GR.
KEGG	ade:Adeh_1044.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0162.
HOGENOM	HBG288125.
OMA	TEFAYQV.
PhylomeDB	Q2IPT7.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1044-MONOMER.
Family and domain databases
HAMAP	MF_02006. Tyr_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_ANADE

Accession

Primary (citable) accession number: Q2IPT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)