Reviewed, UniProtKB/Swiss-Prot Q2INL1 (CHEB2_ANADE)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 2
    EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	Adeh_0614

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Chemotaxis response regulator protein-glutamate methylesterase 2 HAMAP MF_00099		PRO_0000264257
Regions
	Domain	11 – 126	116	Response regulatory
	Domain	152 – 341	190	CheB-type methylesterase
Sites
	Active site	166	1	By similarity
	Active site	193	1	By similarity
	Active site	285	1	By similarity
Amino acid modifications
	Modified residue	62	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2INL1-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 8DEF2F328BB20AF4	FASTA	341	35,782
	10 20 30 40 50 60 MSLAGGDRPI RVLVADDSEL FRELLARVVA AEPGFEVAAV AADGDAAAAM ARALRPDVVT 70 80 90 100 110 120 MDLHMPDADG YSGIARIMAE TPTPILVLTA NPTEAAGFRA LSLGALDILE KPSATADLGE 130 140 150 160 170 180 YGRLIRSRLR LLAGVKVIRH LRGLRERRDA APARAARVEV VVIGASLGGP RALAAVLRGL 190 200 210 220 230 240 PPDFPAPIAV VQHIADGFTA GLAGWLAQES RLDVREARHG DPLRAGRVLI APSGRHLVLG 250 260 270 280 290 300 EGVARLSDAP PVDTFRPSVT PLFTSAARQY GRRCCGVLLT GMGRDGAEGL RVIKDAGGPT 310 320 330 340 LAQDEATSAV FGMARAAVEL GAVDRVLPVD EIPRALRELT R

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC80390.1.
RefSeq	YP_463827.1.
3D structure databases
SMR	Q2INL1. Positions 8-339.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2INL1.
Genome annotation databases
GeneID	3888053.
GenomeReviews	Gene locus Adeh_0614 in contig CP000251_GR.
KEGG	ade:Adeh_0614.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	DEASCAI.
PhylomeDB	Q2INL1.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_0614-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_ANADE

Accession

Primary (citable) accession number: Q2INL1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	March 7, 2006
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)