Reviewed, UniProtKB/Swiss-Prot Q2IMG0 (PROA_ANADE)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	Adeh_0212

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Gamma-glutamyl phosphate reductase		PRO_0000252563

Sequences

	Sequence		Length	Mass (Da)
	Q2IMG0-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: EA1F4626CCDB8EEB	FASTA	427	45,377
	10 20 30 40 50 60 MRKEKSLGLA AEMRKLAEAS REAARALSHA DPRRKDAALR AAAEAIGRRE RRILSENARD 70 80 90 100 110 120 VAAARAAGQN AAYLDRLKLD PKRLAGIAAS LREIAGLRDP VGEVTASWRR PNGLEIRKVR 130 140 150 160 170 180 IPLGVVLMVY EARPNVTVDA AALCLKSGNA AILRPGSDAL RSSLALAEAF AEGLEKAGLP 190 200 210 220 230 240 AASAQVVPTP DREATYELLA LDDLIDLAIP RGGPSLIRAV AERSRVPVLK HYQGVCHLYL 250 260 270 280 290 300 DASAPPQQAV DLALNGKVQR PGVCNATECL LVHRAAAGKL LPPVGRALAD AGVELRCDPT 310 320 330 340 350 360 ALTILKRAGV AAVPARPDDF GKEFLDKILA VRVVADLDGA LDHIARYGSL HTEAIVTRDL 370 380 390 400 410 420 ASARRFQREV DASAVMVNAS TRFNDGGELG LGAEIGISTT KLHAFGPMGL AELTTQKFLV EGEGHVR

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC79989.1.
RefSeq	YP_463426.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3886294.
GenomeReviews	Gene locus Adeh_0212 in contig CP000251_GR.
KEGG	ade:Adeh_0212.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IMG0.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_0212-MON.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_ANADE

Accession

Primary (citable) accession number: Q2IMG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	March 7, 2006
Last modified:	November 25, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)