Reviewed, UniProtKB/Swiss-Prot Q2ILX2 (SYP2_ANADE)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase 2
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase 2
      Short name=ProRS 2

Gene names

Name:	proS2
Ordered Locus Names:	Adeh_0024

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Prolyl-tRNA synthetase 2 HAMAP MF_01571		PRO_0000249114

Sequences

	Sequence		Length	Mass (Da)
	Q2ILX2-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 5E835CE43B0EED0B	FASTA	510	57,291
	10 20 30 40 50 60 MSDASAETAI RPTRAENFSE WYLEVIKAAD LAESSSVRGC MVIKPWGYAL WEHVQRVLDG 70 80 90 100 110 120 MFKATGHVNA YFPLFIPLSL LEKEAAHVEG FAKECAVVTH HRLEARDGKL VPVGELEEPL 130 140 150 160 170 180 IVRPTSETII GESFARWVQS YRDLPLLINQ WANVVRWEMR TRMFLRTAEF LWQEGHTAHA 190 200 210 220 230 240 TEPEAIDETM QMLGIYARFA EEWMALPVVQ GEKTESERFP GAVRTYCIEA MMQDRKALQA 250 260 270 280 290 300 GTSHFLGQNF AKASGIQFQD EKGTLTHAWT TSWGLSTRMI GAMIMTHGDD DGMVCPPRLA 310 320 330 340 350 360 PQQVVIIPVI QKPEVREQVL AWCTALKREL EAQTYAGAPV RVHLDARDLQ GAKKSWEWIK 370 380 390 400 410 420 KGTPVRLEVG PRDIEKGAVF MGRRDRKPRE KQSVPRAELV AGVGALLQEI QDALLERART 430 440 450 460 470 480 MRAAHTRVID TKDEFYAYFT PPPTRRPNDP TPIHGGFALA HFAGDPAVEA RIKEDLGVTV 490 500 510 RCIPLEPGEP GTCAFTGQPS PKRVVWAKSY

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC79802.1.
RefSeq	YP_463239.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3890389.
GenomeReviews	Gene locus Adeh_0024 in contig CP000251_GR.
KEGG	ade:Adeh_0024.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2ILX2.
OMA	Q2ILX2. CIEAMMQ.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_0024-MON.
Family and domain databases
HAMAP	MF_01571. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004499. Pro-tRNA-synth_IIa_pro-type. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP2_ANADE

Accession

Primary (citable) accession number: Q2ILX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)