Reviewed, UniProtKB/Swiss-Prot Q2IKQ7 (PUR9_ANADE)

Names and origin

Protein names

Recommended name:
    Bifunctional purine biosynthesis protein purH
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazolecarboxamide formyltransferase
              EC=2.1.2.3
        Alternative name(s):
            AICAR transformylase
    2- Recommended name:
            IMP cyclohydrolase
              EC=3.5.4.10
        Alternative name(s):
            Inosinicase
            IMP synthetase
            ATIC

Gene names

Name:	purH
Ordered Locus Names:	Adeh_2468

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity.
Sequence similarities	Belongs to the purH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	Bifunctional purine biosynthesis protein purH HAMAP MF_00139		PRO_1000018834

Sequences

	Sequence		Length	Mass (Da)
	Q2IKQ7-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 81A77AAAD34769E7	FASTA	524	55,984
	10 20 30 40 50 60 MTRRALVSVS DKTGLVPFAR RLAALGVELL STGGTQKALA EAGVPVTGVG DYTQAPEILG 70 80 90 100 110 120 GRVKTLHPRV HGGILYRRGL ASDEADVKAR DIPPIDLVVV NLYPFREAVA AGKPFETCVE 130 140 150 160 170 180 EIDIGGPTMV RSAAKNSAHV GVVVDPADYD KVAAELEATR ALSDATRFYL MKKAFAHTAA 190 200 210 220 230 240 YDAAISEYLT ARETPEAAPA HFPATLAAVY TKAYDLRYGE NPHQAGAFYR AAREPEEPSV 250 260 270 280 290 300 AFAQVLQGKE LSYNNLLDLQ AALAGVMEFD ETACVVIKHN TPCGVSTGRT AGEAFARARE 310 320 330 340 350 360 CDPVSAFGGI VALNRPVDEA TASELTSLFL ECVIAPGYDA AARAALAVKK NLRLLEAPRL 370 380 390 400 410 420 GAARATWRRR PEEGRELRSI PGGLLVMDRD LGSVRREDCK VMTKRAPTEQ EWKDLLFAWK 430 440 450 460 470 480 VVKHVKSNAI VFAKDDRTVA IGGGQTSRVE SVKTAVMKAA LDVRGSSVGS DAFFPFADGV 490 500 510 520 EEIIKAGATA IIQPGGSMRD AEVIAAADKA GIAMVATGMR HFRH

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC82238.1.
RefSeq	YP_465675.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3890166.
GenomeReviews	Gene locus Adeh_2468 in contig CP000251_GR.
KEGG	ade:Adeh_2468.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IKQ7.
OMA	Q2IKQ7. VVKHVKS.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_2468-MON.
Family and domain databases
HAMAP	MF_00139. [Tree]
InterPro	IPR002695. AICARFT_IMPCHas. IPR013982. AICARFT_IMPCHas_formly. IPR011607. MGS. [Graphical view]
PANTHER	PTHR11692. AICARFT_IMPCHas. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. [Graphical view]
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_ANADE

Accession

Primary (citable) accession number: Q2IKQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)