Reviewed, UniProtKB/Swiss-Prot Q2IK16 (MDH2_ANADE)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase 2
    EC=1.1.1.37

Gene names

Name:	mdh2
Ordered Locus Names:	Adeh_2226

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Malate dehydrogenase 2 HAMAP MF_00487		PRO_0000241940
Regions
	Nucleotide binding	10 – 15	6	NAD By similarity
	Nucleotide binding	119 – 121	3	NAD By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	34	1	NAD By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	96	1	NAD By similarity
	Binding site	121	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IK16-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 5DFE384EA886C555	FASTA	316	33,020
	10 20 30 40 50 60 MARSKIALIG GGQIGGVLAQ LAALRELGDV VLFDIVEGLP QGKTLDIAEA APVDGFDVSL 70 80 90 100 110 120 KGTNTYEDIK GADVVIVTAG LPRKPGMSRD DLIAVNSKIM TTVAEGIKQY APNAFVIVIS 130 140 150 160 170 180 NPLDAMVTLC QRITGFPHNR VVGQAGVLDS ARFAAFIAWE LGVSVKDVTA VTLGGHGDDM 190 200 210 220 230 240 VPLVRYTSVC GVPVMELLEQ KYGAAKAAEV MAAMVKRTRG AGGEVVALLK TGSAFYSPAS 250 260 270 280 290 300 SAIAMAESFL KDQKRVLPTC AFLKGEFGVD GLYVGVPVVI GAGGAERVLQ LKLNAEEQAM 310 MDKSVKAVKD LVATLK

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC81996.1.
RefSeq	YP_465433.1.
3D structure databases
SMR	Q2IK16. Positions 3-315.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IK16.
Genome annotation databases
GeneID	3889615.
GenomeReviews	Gene locus Adeh_2226 in contig CP000251_GR.
KEGG	ade:Adeh_2226.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HBG566126.
OMA	EINARIM.
PhylomeDB	Q2IK16.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_2226-MONOMER.
Family and domain databases
HAMAP	MF_00487. Malate_dehydrog_3. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_NAD-dep_bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH2_ANADE

Accession

Primary (citable) accession number: Q2IK16

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)