Reviewed, UniProtKB/Swiss-Prot Q2IJN4 (TDH_ANADE)

Names and origin

Protein names

Recommended name:
    L-threonine 3-dehydrogenase
    EC=1.1.1.103

Gene names

Name:	tdh
Ordered Locus Names:	Adeh_2095

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH. HAMAP MF_00627
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Amino-acid degradation; L-threonine degradation via oxydo-reductase pathway; glycine from L-threonine: step 1/2. HAMAP MF_00627
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW threonine catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-threonine 3-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	L-threonine 3-dehydrogenase HAMAP MF_00627		PRO_1000051614
Sites
	Metal binding	39	1	Zinc 1; catalytic By similarity
	Metal binding	64	1	Zinc 1; catalytic By similarity
	Metal binding	94	1	Zinc 2 By similarity
	Metal binding	97	1	Zinc 2 By similarity
	Metal binding	100	1	Zinc 2 By similarity
	Metal binding	108	1	Zinc 2 By similarity
	Metal binding	149	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IJN4-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 1EA58B8901D05EF0	FASTA	345	38,025
	10 20 30 40 50 60 MKALVKAKRE EGIWMQHDVP VPEVGVHDVM IRVTKSAICG TDVHIYNWDE WSQKTVPVPM 70 80 90 100 110 120 VVGHEYVGRV ERVGAEVEAF RPGERVSGEG HVTCGFCRNC RAGRRHLCRH TVGVGVNRPG 130 140 150 160 170 180 SFAEYVVIPA DNVYRIPDDI PDDIAAIFDP FGNATHTALS FDLVGEDVLV TGAGPIGVMA 190 200 210 220 230 240 AAIARHVGAR HVVVTDVNDY RLDLARRMGA SRAVNVARED LRAVMSELGM REGFDVGLEM 250 260 270 280 290 300 SGNGRAFRQL LEVMNHGGRI ALLGIMPGPE PIDWSQVVFK GLQLKGVYGR EMYETWYKMV 310 320 330 340 AMLQSGLDLS AVVTHRFSID DFQQGFDVMR SGRSGKVVLD WGVAR

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC81865.1.
RefSeq	YP_465302.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IJN4.
Genome annotation databases
GeneID	3888333.
GenomeReviews	Gene locus Adeh_2095 in contig CP000251_GR.
KEGG	ade:Adeh_2095.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IJN4.
OMA	FKAITIK.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_2095-MON.
Family and domain databases
HAMAP	MF_00627. [Tree]
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. IPR004627. L-Threonine_3-DHase. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
ProDom	PD040557. GroES_related. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00692. tdh. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TDH_ANADE

Accession

Primary (citable) accession number: Q2IJN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)