Reviewed, UniProtKB/Swiss-Prot Q2IJI0 (ACEK_ANADE)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	Adeh_2039

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity. HAMAP MF_00747
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity HAMAP MF_00747.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288280
Regions
	Nucleotide binding	316 – 322	7	ATP By similarity
Sites
	Active site	372	1	By similarity
	Binding site	337	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IJI0-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 29465DA3327695B2	FASTA	586	65,597
	10 20 30 40 50 60 MLDERGALAR GAAEAIRAGY EAYQAERARI TARARGRFEA RDWAGAQRDA RERLDLRDGV 70 80 90 100 110 120 VHRTVGEVRA ELGGAVQDRE VWRRAKEAFE AVAAARPDAE IAGSFFNSVT RRVLTTVGVD 130 140 150 160 170 180 PAIEFLAADA PPPREDPPQH RAFAREATTE ALLARILRAA PISAPFEDLA RDARLAALEL 190 200 210 220 230 240 DAHVRGLPDR QPIDAVELAR PVFYRGKGAY LVGRIRRGRH LTPLVLALAH GDRGVALDAV 250 260 270 280 290 300 LFTEEDVSIV FGFTRSYFHV ALERPRAMVA FLSTLLPLKR RSELYTGLGY HKHGKAELYR 310 320 330 340 350 360 EVAQHLAEGD DRFVPARGDR GLVMCVFTLP GLDVIFKVIR DRFAPPKQTT RREVMDRYRH 370 380 390 400 410 420 VFRHDRAGRL VDAQEYEHLA FPAARFSPAL LEELRTECGD GVRVAGGEVA IRHLYAERRV 430 440 450 460 470 480 TPLNLFVREA DEWTARQAVL DFGCALRDLA ATDTFPGDLL LKNFGVTRHG RVIFYDYDEL 490 500 510 520 530 540 TRVTDCNFRD LPGAGPGDGD DGWGGGPDAG YDGGDPPFYV GPADVFPEEL LPFLGLTGRL 550 560 570 580 REVFLRAHGE LLTGRWWRDI QARLRAGEIV DIFPYREEQR LRHAHP

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC81809.1.
RefSeq	YP_465246.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IJI0.
Genome annotation databases
GeneID	3887128.
GenomeReviews	Gene locus Adeh_2039 in contig CP000251_GR.
KEGG	ade:Adeh_2039.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4579.
HOGENOM	HBG298593.
OMA	RMTPLNI.
PhylomeDB	Q2IJI0.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_2039-MONOMER.
Family and domain databases
HAMAP	MF_00747. AceK. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProDom	PD043552. Isocitrate_DH_AceK. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ACEK_ANADE

Accession

Primary (citable) accession number: Q2IJI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 7, 2006
Last modified:	January 19, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)