Reviewed, UniProtKB/Swiss-Prot Q2IJC6 (GLO2_ANADE)

Names and origin

Protein names

Recommended name:
    Hydroxyacylglutathione hydrolase
    EC=3.1.2.6
Alternative name(s):
    Glyoxalase II
      Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Adeh_1984

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: InterPro hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_0000309624
Sites
	Metal binding	52	1	Zinc 1 By similarity
	Metal binding	54	1	Zinc 1 By similarity
	Metal binding	56	1	Zinc 2 By similarity
	Metal binding	57	1	Zinc 2 By similarity
	Metal binding	109	1	Zinc 1 By similarity
	Metal binding	126	1	Zinc 1 By similarity
	Metal binding	126	1	Zinc 2 By similarity
	Metal binding	166	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IJC6-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: CF90897C32CCA4E2	FASTA	255	26,228
	10 20 30 40 50 60 MIFDRRRYGK DNYTYLLAAG GDAALVDPGD PDAALALAAA HGVRPRWILH THGHADHTGG 70 80 90 100 110 120 TAAVARALGA QVLGHGGDAA RYRPDVDLAG RAEVALGALA LRVHPVPGHT PGSVLLEWEG 130 140 150 160 170 180 RLLTGDTLFW AGCGNCRHGG DPARLAESFL GPIARLDGGL EVHPGHDYAA PNLAFALALE 190 200 210 220 230 240 PDGAAARARL AEVEAAHAAG LEPAPSTLAG ERAVNPFLRL DAPGVAAAVA RAEPSAAAAG 250 PARRFVALRA LRDRA

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC81755.1.
RefSeq	YP_465192.1.
3D structure databases
HSSP	HSSP built from PDB template 2GCU based on UniProtKB Q9C8L4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IJC6.
Genome annotation databases
GeneID	3887208.
GenomeReviews	Gene locus Adeh_1984 in contig CP000251_GR.
KEGG	ade:Adeh_1984.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	MIFDRRR.
PhylomeDB	Q2IJC6.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1984-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001018. Beta-lactamase_class-B_CS. IPR001279. Blactmase-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLO2_ANADE

Accession

Primary (citable) accession number: Q2IJC6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	March 7, 2006
Last modified:	January 19, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)