ID   SYT_ANADE               Reviewed;         650 AA.
AC   Q2IJC0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-MAR-2006, sequence version 1.
DT   16-JUN-2009, entry version 28.
DE   RecName: Full=Threonyl-tRNA synthetase;
DE            EC=6.1.1.3;
DE   AltName: Full=Threonine--tRNA ligase;
DE            Short=ThrRS;
GN   Name=thrS; OrderedLocusNames=Adeh_1982;
OS   Anaeromyxobacter dehalogenans (strain 2CP-C).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Anaeromyxobacter.
OX   NCBI_TaxID=290397;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C.,
RA   Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M.,
RA   Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S.,
RA   Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P.;
RT   "Complete sequence of Anaeromyxobacter dehalogenans 2CP-C.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + L-threonine + tRNA(Thr) = AMP +
CC       diphosphate + L-threonyl-tRNA(Thr).
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; CP000251; ABC81753.1; -; Genomic_DNA.
DR   RefSeq; YP_465190.1; -.
DR   GeneID; 3887205; -.
DR   GenomeReviews; CP000251_GR; Adeh_1982.
DR   KEGG; ade:Adeh_1982; -.
DR   HOGENOM; Q2IJC0; -.
DR   OMA; Q2IJC0; NTKIAIG.
DR   BioCyc; ADEH290397:ADEH_1982-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004829; F:threonine-tRNA ligase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006435; P:threonyl-tRNA aminoacylation; IEA:HAMAP.
DR   HAMAP; MF_00184; -; 1.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb_cons-reg.
DR   InterPro; IPR006195; aa-tRNA-synth_II_cons-reg.
DR   InterPro; IPR004154; Anticodon_bd.
DR   InterPro; IPR012675; b-grasp_ferredoxin-like.
DR   InterPro; IPR004095; TGS.
DR   InterPro; IPR002320; Thr-tRNA-synth_IIa.
DR   InterPro; IPR018158; Thr-tRNA-synth_IIa_cons-reg.
DR   InterPro; IPR012947; tRNA_SAD.
DR   Gene3D; G3DSA:3.40.50.800; Anticodon_bd; 1.
DR   Gene3D; G3DSA:3.10.20.30; Ferredoxin_fold; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF02824; TGS; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   Pfam; PF07973; tRNA_SAD; 1.
DR   PRINTS; PR01047; TRNASYNTHTHR.
DR   TIGRFAMs; TIGR00418; thrS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis; Zinc.
FT   CHAIN         1    650       Threonyl-tRNA synthetase.
FT                                /FTId=PRO_1000020336.
FT   REGION      248    548       Catalytic.
FT   METAL       349    349       Zinc; catalytic (By similarity).
FT   METAL       400    400       Zinc; catalytic (By similarity).
FT   METAL       525    525       Zinc; catalytic (By similarity).
SQ   SEQUENCE   650 AA;  72874 MW;  8D12CC9FDCAECD33 CRC64;
     MSDLVKVTLP DGSQKEAPRG TPVIDFVKGQ IGAGLAKAAY FAKLDGAPVD LSRALDRDAR
     LEIVTTRSPE ALEVARHDAA HVMASVVQKL YPGTQVTIGP AIEDGFYYDF ARETPFTPED
     LEKIEKATNE AIKADLPFVR SEISMEAALA LFEGMGERYK VEIVKDIAAK GAKTLTLYKH
     GDWVDFCLGP HGPSTGRIGV VKLLNVAGAY WRGDAKNAML QRIYGTAFFD KKELDAHLAK
     LEEVKKRDHR RLGPQLGLFT FHEYAPGAPF WLPAGTVLYN VLEDAMRRLV LKNGYQEVKT
     PLLFNKRLWE TSGHWGKYRE NMFLVVDSES DPALALEDRC SFSLKPMNCP SHHLIYRMDK
     RSYRELPVRY FTTDALHRNE ASGSLGGLTR VRQFEQDDAH IYLREEQVTD EVLRIFELMK
     VVYGAFGLGF EATFSTRPEQ RIGDDALWDR AEALLRKSLD ATGLKWTLNP GDGAFYGPKI
     DMLVTDSLGR RWQTCTIQLD YAAPERFDLT FVGEDNKEHR PVVIHRAIYG SFERFIAILT
     EHYAGAFPAW LAPVQARVVT VSDRFDAWAR EAAAALQARG WRVEVDGSSD KLGAKIRNAQ
     LAKIPFTLVV GEKEVEARGV SPRRHGGEDL KTMPLETFAE LMAREATAPF
//