Reviewed, UniProtKB/Swiss-Prot Q2IJ54 (TRMD_ANADE)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Adeh_1913

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000257388
Regions
	Region	134 – 139	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	115	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IJ54-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: F93A857E36442DCE	FASTA	247	26,889
	10 20 30 40 50 60 MARLEVDILT LFPRMCAGYL GESILGKAQE AGLLAATITD IRDHATGKHR VCDDAPYGGG 70 80 90 100 110 120 AGMVMKPEPL VEAIEAARAR LPGAWVVLTS PRGARLDQAL ARRFAEHGRL ILACGRYEGV 130 140 150 160 170 180 DERVMTAVDM QVSIGDFVLT GGELAALCVV DAAARLVPGV LGNAASADAE SFAGAEGLLE 190 200 210 220 230 240 YPQYTRPPEF RGMRVPEVLL SGDHRRIERW RRREALRATR ERRPDLFTRV SLPESDLRLI DAGDDEL

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC81684.1.
RefSeq	YP_465121.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3887577.
GenomeReviews	Gene locus Adeh_1913 in contig CP000251_GR.
KEGG	ade:Adeh_1913.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IJ54.
OMA	Q2IJ54. HRSVDDT.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1913-MON.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ANADE

Accession

Primary (citable) accession number: Q2IJ54

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)