Q2IIV4 (HUTH_ANADE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histidine ammonia-lyase
Short name=Histidase
EC=4.3.1.3

Gene names

Name:	hutH
Ordered Locus Names:	Adeh_1814

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-histidine = urocanate + NH3. HAMAP MF_00229
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3. HAMAP MF_00229
Subcellular location	Cytoplasm Potential HAMAP MF_00229.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly By similarity. HAMAP MF_00229
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro histidine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histidine ammonia-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Histidine ammonia-lyase HAMAP MF_00229		PRO_0000336582
Amino acid modifications
	Modified residue	144	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	143 ↔ 145		5-imidazolinone (Ala-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IIV4 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 52FF0095D52F0AD5	FASTA	508	53,101
	10 20 30 40 50 60 METLLLDGET LTLEQVRAVA TGAARAALAP AARERVRRSR ALVDARLEDG EAHYGINTGF 70 80 90 100 110 120 GTLAEVRIPR ADLERLQRNL VLSHAAGVGA PLPLAEARAL VLLRANVLAK GVSGIRERTL 130 140 150 160 170 180 ELLLAMLERG VVPVVPERGS VGASGDLAPL AHLALVLIGD GEAFLAPPGA AAPPERLPGG 190 200 210 220 230 240 EALRRAGLEP VVLQPKEGLA LVNGTQAMAA VGTLALLRAE RLAALADLAG AMTLEGLLGS 250 260 270 280 290 300 HRPFAPEIQA ARGQPGQIEA AAHLRALLAG SELNASHQGP GCHKVQDPYS LRCMPQVHGA 310 320 330 340 350 360 ARDGIGFCRG VLAREVNAAT DNPLVFPDTG EIVSGGNFHG QPVALALDVL AVAASHLAAI 370 380 390 400 410 420 SERRVEQLVN PSLSGLPPFL APQHGLNSGF MIAQVTSAAL VSENKVLCHP ASVDSIPSSA 430 440 450 460 470 480 GREDHVSMGM TAALKARQVV ENVRTCLAIE LLVAAQALDL RAPLRPAQRV ADAHARLRER 490 500 VPHLSEDRAL YRDIEAVSRL VDEGALEL

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2CP-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC81586.1.
RefSeq	YP_465023.1. NC_007760.1.
3D structure databases
ProteinModelPortal	Q2IIV4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IIV4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3887396.
GenomeReviews	Gene locus Adeh_1814 in contig CP000251_GR.
KEGG	ade:Adeh_1814.
PATRIC	20919793. VBIAnaDeh31384_1878.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2986.
HOGENOM	HBG510887.
OMA	QKGQIDS.
PhylomeDB	Q2IIV4.
ProtClustDB	PRK09367.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1814-MONOMER.
Family and domain databases
HAMAP	MF_00229. His_ammonia-lyase. [Tree]
InterPro	IPR005921. HutH. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR001106. Phe/His_NH3-lyase. IPR022313. Phe/His_NH3-lyase_AS. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KO	K01745.
Pfam	PF00221. PAL. 1 hit. [Graphical view]
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR01225. HutH. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HUTH_ANADE

Accession

Primary (citable) accession number: Q2IIV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program