Reviewed, UniProtKB/Swiss-Prot Q2IIJ0 (LEXA_ANADE)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	Adeh_1701

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	LexA repressor HAMAP MF_00015		PRO_1000001255
Regions
	DNA binding	28 – 48	21	H-T-H motif By similarity
Sites
	Active site	147	1	For autocatalytic cleavage activity By similarity
	Active site	184	1	For autocatalytic cleavage activity By similarity
	Site	111 – 112	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IIJ0-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: CEC54734F74E898F	FASTA	229	25,071
	10 20 30 40 50 60 MEGLTDRQLE VLRFIASQIE DHGYPPTIRE IGEALDIRST NGVNDHLKAL ERKGYLSRDP 70 80 90 100 110 120 VKSRALIPTS AAREALGGGG EAGSNVVPLV RGPARPGSRM IEIPIVGRVA AGMPILAQER 130 140 150 160 170 180 VEDTVQVDAF LLGTNKKVYG LRVQGDSMIG DGILPGDYVF VKKQLNADDG EIVVAMIDDE 190 200 210 220 ATVKRVYFEG DRVRFQPSNP RMAPIYVRHS DFRSTMILGV VVGVYRKLT

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC81474.1.
RefSeq	YP_464911.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3887649.
GenomeReviews	Gene locus Adeh_1701 in contig CP000251_GR.
KEGG	ade:Adeh_1701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IIJ0.
OMA	Q2IIJ0. KVIGVFR.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1701-MON.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_ANADE

Accession

Primary (citable) accession number: Q2IIJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)