Reviewed, UniProtKB/Swiss-Prot Q2IIB0 (PYRC_ANADE)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	Adeh_1619

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Dihydroorotase		PRO_0000325580
Sites
	Metal binding	63	1	Zinc 1 By similarity
	Metal binding	65	1	Zinc 1 By similarity
	Metal binding	145	1	Zinc 1; via carbamate group By similarity
	Metal binding	145	1	Zinc 2; via carbamate group By similarity
	Metal binding	182	1	Zinc 2 By similarity
	Metal binding	235	1	Zinc 2 By similarity
	Metal binding	310	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	145	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IIB0-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 36351670669BD114	FASTA	433	45,437
	10 20 30 40 50 60 MSDVLFIEGG RVIDPAGGVD GVRTVVIRDG KVAEVAERVE RPRDARVLDA RNRWVTPGFV 70 80 90 100 110 120 DLHVHLREPG QEYKETVATG ARAAVAGGFT AVCAMPNTKP VNDCAAVTEL VLARAAAAGL 130 140 150 160 170 180 ARVYPVGAIS KGSGGEELAE YGELKASGCV ALSDDGRPVM SSALMRRALE YARAFGLPLT 190 200 210 220 230 240 VHEEDLHLVG KGVMHEGAAA TRLGLKGIPS QAEDVMVLRD IALVELTGGR LHVAHVSTAG 250 260 270 280 290 300 AVRAIREAKR RGLPVTGEVT PHHLALTDDD VAASGYSTDF KMNPPLRSAD DVRACREGLA 310 320 330 340 350 360 DGTLDAIATD HAPHSAVEKD VEFDAAANGI VGLETAFSVC LGLVREGALT ERRLVEALTA 370 380 390 400 410 420 GPARVFGLPA GTLARGAAAD VAVLDAAAEW TLDPARLQSK GRNTPWKGRR LAGRCTHTIV 430 GGRIVHEEGK ADR

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC81392.1.
RefSeq	YP_464829.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3888118.
GenomeReviews	Gene locus Adeh_1619 in contig CP000251_GR.
KEGG	ade:Adeh_1619.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IIB0.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1619-MON.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_ANADE

Accession

Primary (citable) accession number: Q2IIB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 7, 2006
Last modified:	September 2, 2008
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)