Reviewed, UniProtKB/Swiss-Prot Q2IIA8 (DNAE2_ANADE)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	Adeh_1612

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	1132 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1132	1132	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000088470

Sequences

	Sequence		Length	Mass (Da)
	Q2IIA8-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 27804FBFF75A32F5	FASTA	1,132	121,076
	10 20 30 40 50 60 MSHAPRYAEL RCKSCFSFLE GASHPEELVG RAAELGLSGL ALADVNGLYG IVRAHAEAKR 70 80 90 100 110 120 QGLPLIVGAE LVVAGLAPGR PARLVLLAQD REGYAGLCRL VTRAHCGEGW TGAPPRRERD 130 140 150 160 170 180 AVAVPFEAVA AGARGLFALY PGADGDAVAR LKDAFGRRAA LAVARHRVAG EEARVLAARS 190 200 210 220 230 240 AGRRLGVPVA VTNDVHTHAR ARQVLQDVLT CVRHGTTVDR AGRRLFPNAE RTLKGPEELA 250 260 270 280 290 300 RLWSDFPEGL AAAADIADQC RFRMEEIRGE HPLPPVVVER GGLAGGVEVA TSSPAQAARD 310 320 330 340 350 360 GARSTTPSLR LRASLPAEPP AAPAPEAPAA AAAPGLAASA ASVAADTGAD RDGALAGMAL 370 380 390 400 410 420 LRELVREGAR WRYGGEPPED VARQLARELD LVESLGYASY FLTVWDVVRF ARSRGILCQG 430 440 450 460 470 480 RGSAANSAVC YVLGITSIDP VRMGLLFERF ISAERGEPPD IDVDFEHERR EEVLQYVYQR 490 500 510 520 530 540 YGRDRAGMVC EVITYRGKSA LRDVGKALGL SLGQVDRLAK LVGSYEDLGQ VGPELLAQAG 550 560 570 580 590 600 LDAADSERVR MTLALARELQ GFPRHLSIHV GGFVITRRPL CETVPIEPAA MPGRTIVQWD 610 620 630 640 650 660 KDDLAELDLL KVDLLGLGML TALSRALALL ARHRPAPASP TPVPHPDALA TIPAEDPEVY 670 680 690 700 710 720 EMLGRADSIG VFQVESRAQM SLAPRLRPRN FYDLVISVAI IRPGPIQGGM IHPYLRRRDG 730 740 750 760 770 780 KEQVRYPYAP LEPVLARTLG VPLFQEQAMR LAVIAAGFTP GEADELRRVM THRRSHEKLA 790 800 810 820 830 840 AMKARLVAGM AERGISGADA EEIFKQLLGF AGYGFPESHA ASFALLVYAS AWLKRYHPAA 850 860 870 880 890 900 FACALLNSQP MGFYAPHTLV EDAKRHGVEV RGVDVGCSGW ESSLEGAAPG RPAAPGEAAV 910 920 930 940 950 960 LRVGLHAIRG LPRAVGEAIL EARAAGPFGS VAELVRRARL SRAWLVRLAE AGALGTLAPD 970 980 990 1000 1010 1020 RRGAVWRSLA VEADGGDLFA GLAPPEPEAA LPEASAADEV SADFTTTGLS VRGHPMALVR 1030 1040 1050 1060 1070 1080 PGLGGDRIRT ARELGRLPDR APVEVAGLVI VRQRPETARG IVFVSLEDET GIANLVVMPD 1090 1100 1110 1120 1130 VYERFRPVVR GAPFLLARGR VERSGKVVNV RVDSVAPLAL APSMDARARD FH

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC81385.1.
RefSeq	YP_464822.1.
3D structure databases
SMR	Q2IIA8. Positions 1-967.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IIA8.
Genome annotation databases
GeneID	3888111.
GenomeReviews	Gene locus Adeh_1612 in contig CP000251_GR.
KEGG	ade:Adeh_1612.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	EGEVTYP.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_1612-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_ANADE

Accession

Primary (citable) accession number: Q2IIA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)