Reviewed, UniProtKB/Swiss-Prot Q2IHC1 (PURL_ANADE)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase 2
    EC=6.3.5.3
Alternative name(s):
    Phosphoribosylformylglycinamidine synthase II
      Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	Adeh_4214

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, purQ and purL. HAMAP MF_00420
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000236647
Regions
	Nucleotide binding	103 – 114	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2IHC1-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 11E3DA4A3FEEBFE4	FASTA	759	80,522
	10 20 30 40 50 60 MTEQITPEIV AQHGLKPDEY QRILEHLGRV PTLTELGVFS VMWSEHCSYK SSRVHLKTFP 70 80 90 100 110 120 TSGPRVLQGP GENAGVVDLG DGLAAAFKME SHNHPSYIEP YQGAATGVGG ILRDVFTMGA 130 140 150 160 170 180 RPIASLNALR FGDPSHPRTA YLLEGVVAGI GGYGNCMGVP TVGGEVAFHP SYNGNCLVNA 190 200 210 220 230 240 FTLGILPADK IFRGTAAGVG NPVMYVGAKT GRDGIHGATM ASAEFDASTE EKRPTVQVGD 250 260 270 280 290 300 PFMEKLLLEA CLELFQTDAV VGIQDMGAAG LTSSSVEMAG RGGNGLDLFL DKVPLREEGM 310 320 330 340 350 360 TPYEILLSES QERMLLVAAE GKEELVRSIC EKWDLDVAVI GRVTASGRWR AHWRGEVVAD 370 380 390 400 410 420 LPVDPLTEGA PKYHRPMTPH PALPALHAFD PATLPEPSDL GAALLRLLAR PTIASKEWVY 430 440 450 460 470 480 RQYDHMVRLV GAVRPGGDAA VVRLAVSHEK HAHKGIALSV GVNGRFCFLD PYLGAMHAVA 490 500 510 520 530 540 ECARNIACVG GEPIAITDCL NFGNPEKPEI MWQFAECVRG IGDACRAFGT PVVSGNVSLY 550 560 570 580 590 600 NETEGQGILP TPTVGMVGLV EPVERTCHST FRAAGDVVAL VGSLQGEVGG SEYLSAEHGK 610 620 630 640 650 660 EAGRPPALDL AREKAVQETV RRAVRAGLLS SAHDCSEGGL AVALAESCMM HEVPADGSTP 670 680 690 700 710 720 AWIGCAVRIP FPVRKDFVLF GEDASRILVS LPKENAARFV ELAQECGAPV IRLGAVGGDR 730 740 750 LEIQGALSVP VEELARAWRD GIPAVLRRDA AHAGAAAPA

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC83978.1.
RefSeq	YP_467415.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IHC1.
Genome annotation databases
GeneID	3888189.
GenomeReviews	Gene locus Adeh_4214 in contig CP000251_GR.
KEGG	ade:Adeh_4214.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IHC1.
OMA	GNCVGVP.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_4214-MON.
Family and domain databases
HAMAP	MF_00420. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_ANADE

Accession

Primary (citable) accession number: Q2IHC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)