Reviewed, UniProtKB/Swiss-Prot Q2IH81 (PYRG_ANADE)

Names and origin

Protein names

Recommended name:
    CTP synthase
    EC=6.3.4.2
Alternative name(s):
    UTP--ammonia ligase
    CTP synthetase

Gene names

Name:	pyrG
Ordered Locus Names:	Adeh_4174

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	555 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen By similarity.
Catalytic activity	ATP + UTP + NH3 = ADP + phosphate + CTP. HAMAP MF_01227
Enzyme regulation	Allosterically activated by GTP, when glutamine is the substrate. Inhibited by CTP By similarity.
Pathway	Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2. HAMAP MF_01227
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the CTP synthase family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW CTP synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 555	555	CTP synthase HAMAP MF_01227		PRO_0000266053
Regions
	Domain	297 – 537	241	Glutamine amidotransferase type-1
	Region	1 – 258	258	Aminator domain HAMAP MF_01227
Sites
	Active site	385	1	Nucleophile By similarity
	Active site	510	1	By similarity
	Active site	512	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IH81-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 27D67205E2F1C146	FASTA	555	61,367
	10 20 30 40 50 60 MVKRGKKTKY LFVTGGVVSS LGKGLSAASI GALLENRGLE VQHLKLDPYI NVDPGTMSPF 70 80 90 100 110 120 QHGEVFVTDD GAETDLDLGH YERFTSAKMT RRNNYTTGRI YQNVIQRERR GEYLGKTVQV 130 140 150 160 170 180 IPHITDEIKA VIREAAGGAD ILIVEVGGTV GDIESLPFLE AIRQMKYDVG EENAVYAHLT 190 200 210 220 230 240 LVPFIAAAGE LKTKPTQHSV KELREIGIQP DLLLCRSDRE IPRDMKDKIA LFCNVDPSAV 250 260 270 280 290 300 FTALDVPSIY EVPLSLHREG LDDKLAELFN IWSRAPRLER WETIVDKVKN PRRGEVRIGI 310 320 330 340 350 360 VGKYVELHES YKSLNEALVH GGIANDARVK LAFIDSTKLE EGDLSDLDKV DAILVPGGFG 370 380 390 400 410 420 IRGTEGKILG VKYAREHKVP FFGICLGLQM AVIEMARNVL GLAGANSLEF DEQTPHPVVT 430 440 450 460 470 480 LMEGQKGVTD KGGTMRLGAY PCALKEGTKA RALYGADLVH ERHRHRFEFN NDYRAQFEAA 490 500 510 520 530 540 GMVFSGVNPD LGLVEMIELP GQHFVGCQFH PEFRSKPFAP HPLFAGFVKA ALEHRDAQQR 550 QPSAEVKKLP VGKNG

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC83938.1.
RefSeq	YP_467375.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3887994.
GenomeReviews	Gene locus Adeh_4174 in contig CP000251_GR.
KEGG	ade:Adeh_4174.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IH81.
OMA	Q2IH81. EFNNAYR.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_4174-MON.
Family and domain databases
HAMAP	MF_01227. [Tree]
InterPro	IPR004468. CTP_synthase. IPR017456. CTP_synthase_N. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11550. PyrG_synth. 1 hit.
Pfam	PF06418. CTP_synth_N. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00337. PyrG. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRG_ANADE

Accession

Primary (citable) accession number: Q2IH81

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	March 7, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)