Reviewed, UniProtKB/Swiss-Prot Q2IH69 (DNLI_ANADE)

Names and origin

Protein names

Recommended name:
    Probable DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	Adeh_4160

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	Probable DNA ligase HAMAP MF_00407		PRO_0000365216
Sites
	Active site	215	1	N6-AMP-lysine intermediate By similarity
	Binding site	213	1	ATP By similarity
	Binding site	220	1	ATP By similarity
	Binding site	235	1	ATP By similarity
	Binding site	264	1	ATP By similarity
	Binding site	304	1	ATP By similarity
	Binding site	376	1	ATP By similarity
	Binding site	382	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IH69-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 96017811B13D10FB	FASTA	513	54,363
	10 20 30 40 50 60 MLLAELAEVS RAVAATPARL EKIARLADAL GRLAPDERAV GASWLAGDLP GGRVGIGAAT 70 80 90 100 110 120 LRAALEAAPP ERSGPGLTVA EVDAALRRIA AVAGPGSGAA RRRELDALLA RAGNPERRFL 130 140 150 160 170 180 AALVLGELRQ GALEGVLADA VAKAAGLPAA EVRRAAMLAG ALPPVAEAAL SEGAAGLARF 190 200 210 220 230 240 RLRVGEPVSP MLAQTAADVD EALRALGGEA ALEWKLDGAR IQAHRDGEAV RVFSRSLRDV 250 260 270 280 290 300 TAAVPEVVAL LRAAPEPRLV LDGEAIALRA DGTPEPFQVT MRRFGRKLDV ERLAPELPLT 310 320 330 340 350 360 AFFFDALVAG GAELLARPER ERWAALARAI PAERRVPRLV TRDPAEARAF LEDALARGQE 370 380 390 400 410 420 GVVAKALDAP YEAGRRGAAW LKVKRAHTLD LVVLAAEWGS GRRRGWLSNL HLGARDPASG 430 440 450 460 470 480 GFVMLGKTFK GMTDAMLAWQ TDRLKALATG PLDAWQVPVR PELVVEVAFD GIQSSPRYPG 490 500 510 GLALRFARVK RYREDKRPED ADTIETVRGL YGG

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC83924.1.
RefSeq	YP_467361.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IH69.
Genome annotation databases
GeneID	3887980.
GenomeReviews	Gene locus Adeh_4160 in contig CP000251_GR.
KEGG	ade:Adeh_4160.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IH69.
OMA	SNLHLGA.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_4160-MON.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_ANADE

Accession

Primary (citable) accession number: Q2IH69

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)