Reviewed, UniProtKB/Swiss-Prot Q2IH17 (ILVD_ANADE)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Adeh_4111

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000000954
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2IH17-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: CCF9228F9C263478	FASTA	560	59,489
	10 20 30 40 50 60 MRSDRIKKGF ERAPHRSLLR ATGLNDGDFE KPFIGIANSH IDIIPGHYYL QEYGRIAKDE 70 80 90 100 110 120 IRKAGGVPFE FNTIGVDDGI AMGHEGMRYS LPSRELIADS IETVMNAHQL DALVCIPNCD 130 140 150 160 170 180 KIVPGMLMGA LRVNVPTVFV SGGPMKAGHL HDGTPIDLNT AFEAVGKRAQ GQITDAELYE 190 200 210 220 230 240 IECQACPSGG SCSGMFTANS MNVLCEAMGV ALPGNGTVLA LTPEREALVR RAARRAVEIA 250 260 270 280 290 300 ADERFRLRNL VNRDAIHNAM VVDMAMGGSS NTVLHMLAIS REAGAPLSLR DIEEIAGKVS 310 320 330 340 350 360 HIAKIAPSLA TVHMEDIHRA GGVPAVLREA ARRGGIVRED ALTVTGETVG ERIRGARTAD 370 380 390 400 410 420 PELIRPLENA YSPVGGLAVL FGNLAAEGAV VKTAGIQPSM RTFTGEAICF DSQDEAIAGI 430 440 450 460 470 480 MGGKVKPGHF VVIRYEGPKG GPGMQEMLSP TSLIMGMGLG ESVALVTDGR FSGATRGACV 490 500 510 520 530 540 GHVSPEAAEG GVIGLVQDGD RITIDVAARA LTVDVSDAEL ARRREGFRPK RRDPGSSWLR 550 560 RYAHLVTNAA NGAVLRSTDL

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC83875.1.
RefSeq	YP_467312.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IH17.
Genome annotation databases
GeneID	3888243.
GenomeReviews	Gene locus Adeh_4111 in contig CP000251_GR.
KEGG	ade:Adeh_4111.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IH17.
OMA	SRKVPCL.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_4111-MON.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_ANADE

Accession

Primary (citable) accession number: Q2IH17

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)