Reviewed, UniProtKB/Swiss-Prot Q2IGK6 (SYP1_ANADE)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase 1
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase 1
      Short name=ProRS 1

Gene names

Name:	proS1
Ordered Locus Names:	Adeh_3947

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Prolyl-tRNA synthetase 1 HAMAP MF_01569		PRO_0000248640

Sequences

	Sequence		Length	Mass (Da)
	Q2IGK6-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 13A94BFAE299D30A	FASTA	575	62,611
	10 20 30 40 50 60 MHAVRYTQAF IPTLKEAPAD AQVASHKLLV RAGFIRQLGA GIYDYLPLAK RSLTKIEAIV 70 80 90 100 110 120 REEMNAIGGQ EFFLPALHPA EIWKESGRWE VMGDNMFRLK DRKGGDYGLG MTHEEIFTAI 130 140 150 160 170 180 ARDELRSYRQ LPQVWYQIQT KFRDEPRPKS GLLRVRQFTM KDAYSFDVDR AGLDKSYEDQ 190 200 210 220 230 240 RQAYEKIFTR CGLDFVAVQA HSGSMGGSES SEFMVRTDAG EDLVAACPRC RYAANTETAT 250 260 270 280 290 300 SRVAAEADGP GLAAPEKFPT PGVVTIEALE QAPHSVPARR QLKTLVYMAD ERPVIAVVRG 310 320 330 340 350 360 DQELNEAKLQ TATGAQVVRP AHAEEIPPLM GAHAGSLGAV RFTRARVVVD PSLADRKDMV 370 380 390 400 410 420 TGANEDGFHL RGVDVRRDLL AHGATLAELR TVKAGEGCPR CDGALEVFKA LEVGHIFKLG 430 440 450 460 470 480 TKYSESMKAT VLDAGGKQVP IVMGSYGIGV ERILAAAIEL HHDDNGIVFP MAIAPFHATV 490 500 510 520 530 540 LTLGPEPELR KAAEEVVAAL GKEGVEVLFD DRDERAGVKF KDADLLGIPI RVAVGKKGLA 550 560 570 AGNVEWKLRR GGAVELVPLG EVARKAAEAV RAATT

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC83711.1.
RefSeq	YP_467148.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IGK6.
Genome annotation databases
GeneID	3887759.
GenomeReviews	Gene locus Adeh_3947 in contig CP000251_GR.
KEGG	ade:Adeh_3947.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	DFVLGPT.
PhylomeDB	Q2IGK6.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_3947-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP1_ANADE

Accession

Primary (citable) accession number: Q2IGK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)