Reviewed, UniProtKB/Swiss-Prot Q2IG43 (TRMB_ANADE)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	Adeh_3785

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	201 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000288117
Sites
	Binding site	33	1	S-adenosyl-L-methionine By similarity
	Binding site	58	1	S-adenosyl-L-methionine By similarity
	Binding site	85	1	S-adenosyl-L-methionine By similarity
	Binding site	108	1	S-adenosyl-L-methionine By similarity
	Binding site	112	1	Substrate By similarity
	Binding site	144	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2IG43-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 3A642EC0FF43E3C5	FASTA	201	22,375
	10 20 30 40 50 60 MIFSIEDDAP IVRDLLPDWR ARFGEAGGRL ELEIGCGHGG FALGFARAFP ERALVGIEQR 70 80 90 100 110 120 RKFAAELAAK GARHGLSNLL VLNGDARLLA PRLFAAGSLA AIHVHFPDPW WKRRHHRRRL 130 140 150 160 170 180 VDDRMSALLL GLLAPGGVLD FRTDVERYAR EAVVRLEEVG FRNAAGPGRF AEAAPDEIPS 190 200 TRERRYLASG EPVWRLRLVK A

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC83551.1.
RefSeq	YP_466988.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3888282.
GenomeReviews	Gene locus Adeh_3785 in contig CP000251_GR.
KEGG	ade:Adeh_3785.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IG43.
OMA	Q2IG43. PDPWWKR.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_3785-MON.
Family and domain databases
HAMAP	MF_01057. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
PANTHER	PTHR23417:SF1. Methyltransf_4. 1 hit.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. CHP91. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_ANADE

Accession

Primary (citable) accession number: Q2IG43

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	March 7, 2006
Last modified:	May 5, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)