Q2IG40 (SYA_ANADE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Adeh_3784

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 905	905	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347488
Sites
	Metal binding	595	1	Zinc Potential
	Metal binding	599	1	Zinc Potential
	Metal binding	696	1	Zinc Potential
	Metal binding	700	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2IG40 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 08BB48C42CE1E0CC	FASTA	905	98,444
	10 20 30 40 50 60 MKTPTAAEIR ELFQRYFEEH GHRRVASSSL VPQNDPTLLF TNAGMVQFKD VFTGRERRDY 70 80 90 100 110 120 SRATTAQKCV RAGGKHNDLE NVGFTARHHT FFEMMGNFSF GDYFKADAIA WAWELVTSPA 130 140 150 160 170 180 WLGIAKDRLA ATVFAGEGTL PWDEEAFELW KAQGVPVERI HKLGAKDNFW AMGDTGPCGP 190 200 210 220 230 240 CSELHYFQGN DVPCAEEQAG RKCQGVACDC DRWLEIWNLV FMQFERGQDG GLTPLPKPSI 250 260 270 280 290 300 DTGAGLERLA AVAQGKRSNY DTDLFRSIIH AVEGLSDKRY DAASDDGVSM RVIADHARAT 310 320 330 340 350 360 TFLVGDGVLP SNEGRGYVLR RIMRRAIRHG KRLGLERPFL AAVCGAVIDE MGAAYPETRE 370 380 390 400 410 420 NRAFIEKVAQ QEEESFRRTL DKGLAILEGE MRKLVPDATH DGKPATPAPD RARPIIDGKV 430 440 450 460 470 480 AFQLYDTFGF PLDLTRVIAA ERGFDVDEQG FDRHMAEQRA RSEWKGSGEQ GVDDLHKQIA 490 500 510 520 530 540 GELGEVKFLG YELPAARAQV KAILANGARV ARAGKGDKVE IVTDATPFYG ESGGQVGDVG 550 560 570 580 590 600 HITGAGLEIR VDDAQRPVPG LVTHVGEVLR GEVAVGDAVE LSVDDRRRDL VRANHSATHL 610 620 630 640 650 660 LQLALREVLG EHVKQAGSVV APDYLRFDFS HFQPVTEEEL AAVERRVNEL VRENAETETA 670 680 690 700 710 720 VLKLEEARHA GAMMIFGEKY GDVVRVVRIG PSKELCGGTH VRRSGDIAFF KIGSEESIAS 730 740 750 760 770 780 GVRRLVAYTG ARAVEVQQRE AEELRRAAAL LKAGALEVSQ KIEQTQRRVK DLERALEEAR 790 800 810 820 830 840 SKAAAAQSGD LAALAKDVGG AKVLAARVEG DGKALRELAD KLRDRLGKGV VALGAEQDGK 850 860 870 880 890 900 AILLVAVTRD LTARLKAGDL VKEAAKLVGG SGGGKPDMAQ AGGSDPAGLE KALEKVAELA ARALA

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2CP-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC83550.1.
RefSeq	YP_466987.1. NC_007760.1.
3D structure databases
ProteinModelPortal	Q2IG40.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IG40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3888281.
GenomeReviews	Gene locus Adeh_3784 in contig CP000251_GR.
KEGG	ade:Adeh_3784.
PATRIC	20923811. VBIAnaDeh31384_3862.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	MFTNSGM.
PhylomeDB	Q2IG40.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_3784-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_ANADE

Accession

Primary (citable) accession number: Q2IG40

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program