Reviewed, UniProtKB/Swiss-Prot Q2IG33 (DDL_ANADE)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	Adeh_3774

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	D-alanine--D-alanine ligase		PRO_0000341050
Regions
	Domain	105 – 302	198	ATP-grasp
	Nucleotide binding	133 – 188	56	ATP By similarity
Sites
	Metal binding	256	1	Magnesium or manganese 1 By similarity
	Metal binding	269	1	Magnesium or manganese 1 By similarity
	Metal binding	269	1	Magnesium or manganese 2 By similarity
	Metal binding	271	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IG33-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 139E9F21B9EE075E	FASTA	308	32,040
	10 20 30 40 50 60 MSTWTGKRVA VLYGGRSSER EVSLRTGAAC AEALRQKGHD VVLVDVDLEV AARLRAERVE 70 80 90 100 110 120 VAFVALHGRW GEDGSIQGLL ESMAIPYTGS GVLASAMGMD KTVSKAIFRS LGLAVADYRV 130 140 150 160 170 180 FPRAAAGAIG VDDLPFGLPC VVKPAGEGSS VGVHLVNAAA ELGPACRDAA GYAGDVIVER 190 200 210 220 230 240 YVKGTEVDVA VLEGKALGAI EIVPANAFYD YAAKYTAGTT KYFYPARIPE AHVRAVMEAA 250 260 270 280 290 300 EAAHRGIGCS GVTRVDFIVA ADGTPYILEV NTLPGMTATS LVPKIAAGLG LSFPDLCDRI LDGAALKA

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC83540.1.
RefSeq	YP_466977.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3888271.
GenomeReviews	Gene locus Adeh_3774 in contig CP000251_GR.
KEGG	ade:Adeh_3774.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IG33.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_3774-MON.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_ANADE

Accession

Primary (citable) accession number: Q2IG33

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	March 7, 2006
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)