Reviewed, UniProtKB/Swiss-Prot Q2IFU3 (PANC_ANADE)

Names and origin

Protein names

Recommended name:
    Pantothenate synthetase
      Short name=PS
    EC=6.3.2.1
Alternative name(s):
    Pantoate--beta-alanine ligase
    Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	Adeh_3681

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Pantothenate synthetase HAMAP MF_00158		PRO_0000305390
Regions
	Nucleotide binding	33 – 40	8	ATP By similarity
	Nucleotide binding	157 – 160	4	ATP By similarity
	Nucleotide binding	194 – 197	4	ATP By similarity
Sites
	Active site	40	1	Proton donor By similarity
	Binding site	70	1	Beta-alanine By similarity
	Binding site	70	1	Pantoate By similarity
	Binding site	163	1	Pantoate By similarity
	Binding site	186	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IFU3-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: F55FC51F1DA2F117	FASTA	289	30,882
	10 20 30 40 50 60 MKTPELITDP AAWQARCTAA REAGTRIALV TTMGYLHEGH LSLMREARRR ADAGGRRGLA 70 80 90 100 110 120 VGTIFVNPTQ FGPTEDLARY PRDLEGDLAK CAAAGLDAVL APSDPALMFA PGHETWVTVE 130 140 150 160 170 180 RASQGLDGAS RPGHFRGVAT VVAKLFNLTR PHVALFGEKD WQQLAVIRAM VRDLAFGIEI 190 200 210 220 230 240 VGMPIVREPD GLALSSRNAY LSPDERRRAL ALSGALAEAR EATARGERDA AALRAGARAR 250 260 270 280 LEAAGGRVDY VELVHPETLA PVARAEPGTV LLLAASFGTT RLIDNGRLP

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC83447.1.
RefSeq	YP_466884.1.
3D structure databases
SMR	Q2IFU3. Positions 5-288.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IFU3.
Genome annotation databases
GeneID	3886456.
GenomeReviews	Gene locus Adeh_3681 in contig CP000251_GR.
KEGG	ade:Adeh_3681.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0414.
HOGENOM	HBG428839.
OMA	MPIQIIG.
PhylomeDB	Q2IFU3.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_3681-MONOMER.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_ANADE

Accession

Primary (citable) accession number: Q2IFU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)