Reviewed, UniProtKB/Swiss-Prot Q2IFU2 (PANB_ANADE)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase
      Short name=KPHMT

Gene names

Name:	panB
Ordered Locus Names:	Adeh_3682

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP MF_00156
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00156
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity. HAMAP MF_00156
Subcellular location	Cytoplasm Potential HAMAP MF_00156.
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156		PRO_0000297214
Regions
	Region	52 – 53	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	194	1	Proton acceptor By similarity
	Metal binding	52	1	Magnesium By similarity
	Metal binding	95	1	Magnesium By similarity
	Metal binding	127	1	Magnesium By similarity
	Binding site	95	1	Alpha-ketoisovalerate By similarity
	Binding site	125	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IFU2-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 6466692963670454	FASTA	305	32,132
	10 20 30 40 50 60 MSSHPPPRKH VNIHELRRMK EAGERIAMVT AYDATAARLV AAAGVDAVLV GDSLGMAVQG 70 80 90 100 110 120 HESTLPVTLD QMVYHSAMVR RGLARGDGRA HLVADMSFGS YQASADEAVK AAMRLVAEGG 130 140 150 160 170 180 AEAVKLEGGA EFGDVIRRIV RAGVPVMGHI GLTPQSVHKM GGYVVQGKDS EKAQQILRDA 190 200 210 220 230 240 RALEAAGCYA LVLECIPSEL ARIVTSQLRI PTIGIGAGPH CDGQVLVLND LLGLDASFTP 250 260 270 280 290 300 KFVKRFGELG AAVEGAVGAY VGEVKARAFP DDAHSFHSAS VRLVPVERHA EEAEEEPPDA IGAPI

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC83448.1.
RefSeq	YP_466885.1.
3D structure databases
SMR	Q2IFU2. Positions 11-274.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IFU2.
Genome annotation databases
GeneID	3886457.
GenomeReviews	Gene locus Adeh_3682 in contig CP000251_GR.
KEGG	ade:Adeh_3682.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0413.
HOGENOM	HBG299908.
OMA	YATPEQT.
PhylomeDB	Q2IFU2.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_3682-MONOMER.
Family and domain databases
HAMAP	MF_00156. PanB. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB_ANADE

Accession

Primary (citable) accession number: Q2IFU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)