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Q2IFT1 (PCKG_ANADE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxykinase [GTP]
Short name=PEP carboxykinase
Short name=PEPCK
EC=4.1.1.32
Alternative name(s):
Phosphoenolpyruvate carboxylase
Gene names
Name:pckG
Ordered Locus Names:Adeh_3676
OrganismAnaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]
Taxonomic identifier290397 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length596 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle By similarity. HAMAP MF_00452

Catalytic activity

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2. HAMAP MF_00452

Cofactor

Binds 1 manganese ion per subunit By similarity. HAMAP MF_00452

Pathway

Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00452

Subunit structure

Monomer By similarity. HAMAP MF_00452

Subcellular location

Cytoplasm By similarity HAMAP MF_00452.

Sequence similarities

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Ontologies

Keywords
   Biological processGluconeogenesis
   Cellular componentCytoplasm
   LigandGTP-binding
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionDecarboxylase
Lyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoenolpyruvate carboxykinase (GTP) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 596596Phosphoenolpyruvate carboxykinase [GTP] HAMAP MF_00452
PRO_1000060286

Regions

Nucleotide binding257 – 2626GTP By similarity
Nucleotide binding499 – 5024GTP By similarity
Region373 – 3753Substrate binding By similarity

Sites

Active site2581 By similarity
Metal binding2141Manganese By similarity
Metal binding2341Manganese By similarity
Metal binding2831Manganese By similarity
Binding site771Substrate By similarity
Binding site2071Substrate; via amide nitrogen By similarity
Binding site2141Substrate By similarity
Binding site2561Substrate By similarity
Binding site3751GTP By similarity
Binding site4061GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2IFT1 [UniParc].

Last modified March 7, 2006. Version 1.
Checksum: E868169319469F89

FASTA59665,789
        10         20         30         40         50         60 
MSTSPAARPT SNPHLLGWVD EMAKLCKPDR VHWCDGSEAE KKRLTDDAVA AKVLIPLDQQ 

        70         80         90        100        110        120 
KWPGCHYHHS NPSDVARVEH LTFICTPTKE QAGPTNNWME PKEAYRKLGA IFDGSMKGRT 

       130        140        150        160        170        180 
MYVVPYVMGP STSPFAKVGI EITDSVYVAL NMGIMARMGK VALDRLGDSN EFNRGLHSVA 

       190        200        210        220        230        240 
DCNPERRFIC HFPQDNTIWS VGSGYGGNAL LGKKCLALRI ASYLAKNEGW LAEHMLILEA 

       250        260        270        280        290        300 
ESPTGEKQYV AAAFPSACGK TNFAMMIPPA AFPGWKIRTV GDDISWMRVG EDGRLWAVNP 

       310        320        330        340        350        360 
ENGYFGVAPG TNRKTNPNAM DSVRKDTIFT NVARTPDGDI WWEGMDHEAP AELIDWKGQP 

       370        380        390        400        410        420 
WKKGSTEKAA HPNSRFTAPA KNNPAISPLV DDPKGVPISA IIFGGRRSTT VPLVLEAFNW 

       430        440        450        460        470        480 
THGVYLGSTM GSETTAAATG QVGVVRRDPM AMLPFIGYDC GSYLQHWLDM QSRIPNPPKI 

       490        500        510        520        530        540 
FLVNWFRKSA EGKFLWPGYG DNMRVLKWML DRAAGRAPAK ETLLGYTPGD AGLDLHGLDV 

       550        560        570        580        590 
SKDAIAAATR IDLGEWEQEL ESQAEWFEKL GKTLPAPLAL QRELLLERVR AARKVK

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References

[1]"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. expand/collapse author list , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P.
Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 2CP-C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000251 Genomic DNA. Translation: ABC83442.1.
RefSeqYP_466879.1. NC_007760.1.

3D structure databases

HSSPHSSP built from PDB template 2FAG based on UniProtKB P21642.
ProteinModelPortalQ2IFT1.
SMRQ2IFT1. Positions 9-589.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ2IFT1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3886451.
GenomeReviewsGene locus Adeh_3676 in contig CP000251_GR.
KEGGade:Adeh_3676.
PATRIC20923597. VBIAnaDeh31384_3755.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1274.
HOGENOMHBG484437.
OMACGYHMGD.
ProtClustDBPRK04210.

Enzyme and pathway databases

BioCycADEH290397:ADEH_3676-MONOMER.

Family and domain databases

HAMAPMF_00452. PEPCK_GTP.
[Tree]
InterProIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
Gene3DG3DSA:3.90.228.20. PEP_carboxykinase_C. 2 hits.
G3DSA:3.40.449.10. PEP_carboxykinase_N. 1 hit.
KOK01596.
PANTHERPTHR11561. PEP_carboxykin. 1 hit.
PfamPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMSSF68923. PEP_carboxykinase_N. 1 hit.
PROSITEPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePCKG_ANADE
AccessionPrimary (citable) accession number: Q2IFT1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 7, 2006
Last modified: January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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