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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Anaeromyxobacter dehalogenans (strain 2CP-C)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateUniRule annotation1
Metal bindingi214ManganeseUniRule annotation1
Metal bindingi234Manganese; via tele nitrogenUniRule annotation1
Binding sitei256SubstrateUniRule annotation1
Active sitei258UniRule annotation1
Metal bindingi283ManganeseUniRule annotation1
Binding sitei375GTPUniRule annotation1
Binding sitei406GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi257 – 262GTPUniRule annotation6
Nucleotide bindingi499 – 502GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciADEH290397:G1G5W-3817-MONOMER
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:Adeh_3676
OrganismiAnaeromyxobacter dehalogenans (strain 2CP-C)
Taxonomic identifieri290397 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeAnaeromyxobacteraceaeAnaeromyxobacter
Proteomesi
  • UP000001935 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000602861 – 596Phosphoenolpyruvate carboxykinase [GTP]Add BLAST596

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi290397.Adeh_3676

Structurei

3D structure databases

ProteinModelPortaliQ2IFT1
SMRiQ2IFT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni205 – 207Substrate bindingUniRule annotation3
Regioni373 – 375Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M Bacteria
COG1274 LUCA
HOGENOMiHOG000191700
KOiK01596
OMAiCWLALTD

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

Q2IFT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSPAARPT SNPHLLGWVD EMAKLCKPDR VHWCDGSEAE KKRLTDDAVA
60 70 80 90 100
AKVLIPLDQQ KWPGCHYHHS NPSDVARVEH LTFICTPTKE QAGPTNNWME
110 120 130 140 150
PKEAYRKLGA IFDGSMKGRT MYVVPYVMGP STSPFAKVGI EITDSVYVAL
160 170 180 190 200
NMGIMARMGK VALDRLGDSN EFNRGLHSVA DCNPERRFIC HFPQDNTIWS
210 220 230 240 250
VGSGYGGNAL LGKKCLALRI ASYLAKNEGW LAEHMLILEA ESPTGEKQYV
260 270 280 290 300
AAAFPSACGK TNFAMMIPPA AFPGWKIRTV GDDISWMRVG EDGRLWAVNP
310 320 330 340 350
ENGYFGVAPG TNRKTNPNAM DSVRKDTIFT NVARTPDGDI WWEGMDHEAP
360 370 380 390 400
AELIDWKGQP WKKGSTEKAA HPNSRFTAPA KNNPAISPLV DDPKGVPISA
410 420 430 440 450
IIFGGRRSTT VPLVLEAFNW THGVYLGSTM GSETTAAATG QVGVVRRDPM
460 470 480 490 500
AMLPFIGYDC GSYLQHWLDM QSRIPNPPKI FLVNWFRKSA EGKFLWPGYG
510 520 530 540 550
DNMRVLKWML DRAAGRAPAK ETLLGYTPGD AGLDLHGLDV SKDAIAAATR
560 570 580 590
IDLGEWEQEL ESQAEWFEKL GKTLPAPLAL QRELLLERVR AARKVK
Length:596
Mass (Da):65,789
Last modified:March 7, 2006 - v1
Checksum:iE868169319469F89
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000251 Genomic DNA Translation: ABC83442.1
RefSeqiWP_011422724.1, NC_007760.1

Genome annotation databases

EnsemblBacteriaiABC83442; ABC83442; Adeh_3676
KEGGiade:Adeh_3676

Similar proteinsi

Entry informationi

Entry nameiPCKG_ANADE
AccessioniPrimary (citable) accession number: Q2IFT1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 7, 2006
Last modified: March 28, 2018
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health