Q2IEX2 (NTPA_ANADE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

Adeh_3360

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_1000068407
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	70 – 71	2	Substrate binding By similarity
Sites
	Metal binding	70	1	Magnesium or manganese By similarity
	Binding site	183	1	Substrate By similarity
	Binding site	211	1	Substrate By similarity
	Binding site	217	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IEX2 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: BDF0A6FB224A2B89	FASTA	235	24,465
	10 20 30 40 50 60 MDLLFGSTNP GKLRELRRLV AGLPLRVVSP DDLGRPLPVV VEDGATFQAN AEKKAVAWAR 70 80 90 100 110 120 WSGLHAVADD SGLCVDALGG APGVHSARWS DLEPEGPASP VCELAGVAEL ELGPVAGRAA 130 140 150 160 170 180 RDERNNDKLL AALSGLPDPR RGARYEAVLA LARPDGTLVG TVTGTCPGRI GHARRGDGGF 190 200 210 220 230 GYDPLFVPAA ELAAGEGARV RTMAELSSDE KDALSHRGEA FRKLLPMLAA LARGA

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 2CP-C.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000251 Genomic DNA. Translation: ABC83127.1.
RefSeq	YP_466564.1. NC_007760.1.
3D structure databases
ProteinModelPortal	Q2IEX2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IEX2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3889350.
GenomeReviews	Gene locus Adeh_3360 in contig CP000251_GR.
KEGG	ade:Adeh_3360.
PATRIC	20922965. VBIAnaDeh31384_3442.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0127.
HOGENOM	HBG697237.
OMA	GEAHNDA.
PhylomeDB	Q2IEX2.
ProtClustDB	CLSK946631.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_3360-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTPA_ANADE

Accession

Primary (citable) accession number: Q2IEX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 7, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program