Reviewed, UniProtKB/Swiss-Prot Q2IEC3 (T23O_ANADE)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	Adeh_3165

Organism

Anaeromyxobacter dehalogenans (strain 2CP-C) [Complete proteome] [HAMAP]

Taxonomic identifier

290397 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Myxococcales › Cystobacterineae › Myxococcaceae › Anaeromyxobacter

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Tryptophan 2,3-dioxygenase		PRO_0000360080
Regions
	Region	13 – 17	5	Substrate binding By similarity
	Region	38 – 42	5	Substrate binding By similarity
Sites
	Metal binding	223	1	Iron (heme axial ligand) By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	111	1	Heme By similarity
	Binding site	237	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2IEC3-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: FCE500FAEA6B7510	FASTA	265	29,720
	10 20 30 40 50 60 MTDPSAHSAA LTYGSYLALD ELLAAQRPRS EEHDELLFIV VHQVYELWFK QVVHELTWLQ 70 80 90 100 110 120 ERLHRGEGGH ALATLKRVLT ILKTVVAQVD VIETMTPRQF TAFRSRLEAA SGFQSAQFRV 130 140 150 160 170 180 LEAMLGRRDD RMLAPYPPDG PGYARIAAAM AAPSLFDSLL RYLATQGFET PVVPEPRPAG 190 200 210 220 230 240 WRQPSEAVQR VLLEVYRADG EAALVCERFV DLDEGVQEWR YRHVKMVERT IGDKPGTGGS 250 260 AGARYLRSTL FTPAFPDLWA VRGAL

References

[1]

"Complete sequence of Anaeromyxobacter dehalogenans 2CP-C." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Kiss H., Schmutz J., Larimer F., Land M. , Kyrpides N., Anderson I., Sanford R.A., Ritalahti K.M., Thomas H.S., Kirby J.R., Zhulin I.B., Loeffler F.E., Richardson P. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000251 Genomic DNA. Translation: ABC82934.1.
RefSeq	YP_466371.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q2IEC3.
Genome annotation databases
GeneID	3888971.
GenomeReviews	Gene locus Adeh_3165 in contig CP000251_GR.
KEGG	ade:Adeh_3165.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q2IEC3.
OMA	QWSVLAT.
Enzyme and pathway databases
BioCyc	ADEH290397:ADEH_3165-MON.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_ANADE

Accession

Primary (citable) accession number: Q2IEC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	March 7, 2006
Last modified:	November 3, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)