Q2IBD4 (CTTB2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cortactin-binding protein 2
Short name=CortBP2

Gene names

Name:

Cttnbp2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1649 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates the dendritic spine distribution of CTTN/cortactin in hippocampal neurons, thus controls dendritic spinogenesis and dendritic spine maintenance. Ref.5
Subunit structure	Interacts with CTTN/cortactin SH3 domain. Interacts with STRN, STRN4/zinedin and MOB4/phocein; this interaction may regulate dendritic spine distribution of STRN and STRN4 in hippocampal neurons. Activation of glutamate receptors weakens the interaction with STRN and STRN4. Ref.5 Ref.6
Subcellular location	Cytoplasm › cell cortex By similarity. Cell projection › dendritic spine. Note: Remains associated with dendritic spines even after glutamate stimulation. Ref.5
Sequence similarities	Contains 6 ANK repeats.

Ontologies

Keywords
Cellular component	Cell projection Cytoplasm
Coding sequence diversity	Alternative splicing
Domain	ANK repeat Coiled coil Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	brain development Inferred from expression pattern Ref.1. Source: RGD
Cellular_component	synaptic vesicle Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q2IBD4-1)

Also known as: CTTNBP2-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q2IBD4-2)

Also known as: CTTNBP2-S;

The sequence of this isoform differs from the canonical sequence as follows:
     626-630: VGAWP → AGHPP
     631-1649: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1649	1649	Cortactin-binding protein 2		PRO_0000422173
Regions
	Repeat	699 – 729	31	ANK 1
	Repeat	733 – 762	30	ANK 2
	Repeat	766 – 795	30	ANK 3
	Repeat	799 – 828	30	ANK 4
	Repeat	832 – 861	30	ANK 5
	Repeat	901 – 931	31	ANK 6
	Coiled coil	118 – 275	158	Potential
Amino acid modifications
	Modified residue	1510	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	626 – 630	5	VGAWP → AGHPP in isoform 2.		VSP_046479
	Alternative sequence	631 – 1649	1019	Missing in isoform 2.		VSP_046480
Experimental info
	Sequence conflict	163	1	R → K in AAC35911. Ref.1
	Sequence conflict	260	1	K → Q in AAC35911. Ref.1
	Sequence conflict	280	1	L → F in AAC35911. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CTTNBP2-L) [UniParc]. Last modified March 7, 2006. Version 1. Checksum: 12C85BF86EB2634B	FASTA	1,649	178,768
	10 20 30 40 50 60 MATDSASCEP DLSRAPGDAE GATAEAAKKE FDVDTLSKSE LRMLLSVMEG ELEARDLVIE 70 80 90 100 110 120 ALRARRKEVF IQERYGRFNL NDPFLALQRD YEAGAGDKEK PVCTNPLSIL EAVMAHCRKM 130 140 150 160 170 180 QERMSAQLAA AESRQKKLEM EKLQLQALEQ EHKKLAAHLE EERGKNKHVV LMLVKECKQL 190 200 210 220 230 240 SGKVVEEAQK LEEVMVKLEE EKKKTSELED QLSAEKQRSA GMEAQLEKQL FEFDTEREQL 250 260 270 280 290 300 RAKLTREEAH TTDLKEEIDK MKKMMEQMKK GNDGKPGLSL PRKTKDKRLA SISVATEGPV 310 320 330 340 350 360 TRSVACQTDV VTESTDPVKK LPLSVPIKPS TGSPLVSTNT KGNVGPSALL IRPGIDRQAS 370 380 390 400 410 420 HSDLGPSPPT ALPSSASRIE ENGPSAGNAP DLSNSTPSTP SGTAPAAAQT LGAAPQNHSQ 430 440 450 460 470 480 APPVHSLHSP CANTHPGLNP RIQAARFRFQ GNANDPDQNG NNTQSPPSRD VSPTSRDNLV 490 500 510 520 530 540 AKQLARNTVT QALSRFTSPQ AGASSRLGAS PGGDAGTCPP VGRTGLKTPG AARVDRGNPP 550 560 570 580 590 600 PIPPKKPGLS QTPSPPHPQL RASNAGAKVD NKIVASPPST LPQGTKVVNE ENVPKSSSPQ 610 620 630 640 650 660 LPPKPSIDLT VASAGCPVSA LATSQVGAWP AETPGLNQPA CTDSSLVIPT TVAFRSSINP 670 680 690 700 710 720 VSASSRSPGA SDSLLVTASG WSPSLTPLLM SGGPAPLAGR PTLLQQAAAQ GNVTLLSMLL 730 740 750 760 770 780 NEEGLDINYC CEDSHSALYS AAKNGHTDCV RLLLNAEARV DAADKNGFTP LCVAAAQGHF 790 800 810 820 830 840 ECIELLTAYN ANINHSAAGG QTPLYLACKT GNKECIKLLL EAGTDRSIKT RDGWTPIHAA 850 860 870 880 890 900 VDTGNVDSLK LLMYHRVPAP GNSLSAEEPK SGLFSLNGGE SPPGSSKPVV PADLINHADK 910 920 930 940 950 960 EGWTAAHIAA SKGFKNCLEI LCRHGGLEPE RRDKCNRTVH DVATDDCKHL LENLNALKIP 970 980 990 1000 1010 1020 LRISVGEIQP SNDGSDDFEC EHTICTLNIR KQTSWEDFSK AVSQALTNHF QAISSDGCWG 1030 1040 1050 1060 1070 1080 LEDGTLNNTT DSCIGLGTSS IQSIMLGSIP WSTGQSFSQS PWDFMKKKKV EQVTVLLSGP 1090 1100 1110 1120 1130 1140 QEGCLSSVTY TSMIPLQMLQ NYLRLVEQYH NVIFHGPEGS LQDYIANQLA LCMKHRQMAA 1150 1160 1170 1180 1190 1200 GFPCEIVRAE VDSGFSKEQL VDVFISNACL IPVKQFPVKK KIIVILENLE KSSLSELLGD 1210 1220 1230 1240 1250 1260 FLAPLENRST ESPCTFQKGN GTSECYYFHE NCFLLGTLAK ACLQGSDLLV QQHFRWVQLR 1270 1280 1290 1300 1310 1320 WDCEPSQGLL QRFLRRKAVS KFRGQLPAPC DPVCKIVDWV ISVWRQLNSC LARLGTPEAL 1330 1340 1350 1360 1370 1380 LGPKYFLSCP VVPGHAQATV KWMSKLWNAI IAPRVQEAIL SRAAMNKQPG ARQTASKKHP 1390 1400 1410 1420 1430 1440 SQGQQAVVRA ALSILLNKAI LHGCPLPRTE LDQQIADFKG GSFPLSIVSS YSKKKGESAA 1450 1460 1470 1480 1490 1500 WRKVNTSPRK KPGHFSSPMW NKPDLKHEGM RNKSVPHLNI NRSSSLSKQQ SLENDLSMTL 1510 1520 1530 1540 1550 1560 TLDHRLSLGS DDEADLVKEL QSMCSSKSES DISKIADSRE DLRTFDSSRT NPVTSAPVNL 1570 1580 1590 1600 1610 1620 RMPVPQKEAS PLSSHQTTEC SNSKSKTELG VSRVKSFLPV PRSKVAQCSQ NTKRSSSSSS 1630 1640 NTRQLEINNN SKEENWNVDK HEHVEKRNK
	Isoform 2 (CTTNBP2-S) [UniParc]. Checksum: CE583C3D3DCBEE8E	FASTA	630	67,351
	10 20 30 40 50 60 MATDSASCEP DLSRAPGDAE GATAEAAKKE FDVDTLSKSE LRMLLSVMEG ELEARDLVIE 70 80 90 100 110 120 ALRARRKEVF IQERYGRFNL NDPFLALQRD YEAGAGDKEK PVCTNPLSIL EAVMAHCRKM 130 140 150 160 170 180 QERMSAQLAA AESRQKKLEM EKLQLQALEQ EHKKLAAHLE EERGKNKHVV LMLVKECKQL 190 200 210 220 230 240 SGKVVEEAQK LEEVMVKLEE EKKKTSELED QLSAEKQRSA GMEAQLEKQL FEFDTEREQL 250 260 270 280 290 300 RAKLTREEAH TTDLKEEIDK MKKMMEQMKK GNDGKPGLSL PRKTKDKRLA SISVATEGPV 310 320 330 340 350 360 TRSVACQTDV VTESTDPVKK LPLSVPIKPS TGSPLVSTNT KGNVGPSALL IRPGIDRQAS 370 380 390 400 410 420 HSDLGPSPPT ALPSSASRIE ENGPSAGNAP DLSNSTPSTP SGTAPAAAQT LGAAPQNHSQ 430 440 450 460 470 480 APPVHSLHSP CANTHPGLNP RIQAARFRFQ GNANDPDQNG NNTQSPPSRD VSPTSRDNLV 490 500 510 520 530 540 AKQLARNTVT QALSRFTSPQ AGASSRLGAS PGGDAGTCPP VGRTGLKTPG AARVDRGNPP 550 560 570 580 590 600 PIPPKKPGLS QTPSPPHPQL RASNAGAKVD NKIVASPPST LPQGTKVVNE ENVPKSSSPQ 610 620 630 LPPKPSIDLT VASAGCPVSA LATSQAGHPP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of cortactin isoforms and a novel cortactin-binding protein, CBP90." Ohoka Y., Takai Y. Genes Cells 3:603-612(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"Comparative analyses of multi-species sequences from targeted genomic regions." Thomas J.W., Touchman J.W., Blakesley R.W., Bouffard G.G., Beckstrom-Sternberg S.M., Margulies E.H., Blanchette M., Siepel A.C., Thomas P.J., McDowell J.C., Maskeri B., Hansen N.F., Schwartz M.S., Weber R.J., Kent W.J., Karolchik D., Bruen T.C., Bevan R. , Cutler D.J., Schwartz S., Elnitski L., Idol J.R., Prasad A.B., Lee-Lin S.-Q., Maduro V.V.B., Summers T.J., Portnoy M.E., Dietrich N.L., Akhter N., Ayele K., Benjamin B., Cariaga K., Brinkley C.P., Brooks S.Y., Granite S., Guan X., Gupta J., Haghighi P., Ho S.-L., Huang M.C., Karlins E., Laric P.L., Legaspi R., Lim M.J., Maduro Q.L., Masiello C.A., Mastrian S.D., McCloskey J.C., Pearson R., Stantripop S., Tiongson E.E., Tran J.T., Tsurgeon C., Vogt J.L., Walker M.A., Wetherby K.D., Wiggins L.S., Young A.C., Zhang L.-H., Osoegawa K., Zhu B., Zhao B., Shu C.L., De Jong P.J., Lawrence C.E., Smit A.F., Chakravarti A., Haussler D., Green P., Miller W., Green E.D. Nature 424:788-793(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[3]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Cortactin-binding protein 2 modulates the mobility of cortactin and regulates dendritic spine formation and maintenance." Chen Y.K., Hsueh Y.P. J. Neurosci. 32:1043-1055(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CTTN, SUBCELLULAR LOCATION.
[6]	"CTTNBP2, but not CTTNBP2NL, regulates dendritic spinogenesis and synaptic distribution of the striatin-PP2A complex." Chen Y.K., Chen C.Y., Hu H.T., Hsueh Y.P. Mol. Biol. Cell 23:4383-4392(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH STRN; STRN4 AND MOB4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF053768 mRNA. Translation: AAC35911.1. DP000027 Genomic DNA. Translation: AAR16316.1. AC087251 Genomic DNA. No translation available. AC111378 Genomic DNA. No translation available. AC119088 Genomic DNA. No translation available. CH473959 Genomic DNA. Translation: EDM15128.1.
IPI	IPI00370652.
RefSeq	NP_001107873.1. NM_001114401.1.
UniGene	Rn.16244.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q2IBD4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000011135; ENSRNOP00000011135; ENSRNOG00000008440.
GeneID	282587.
KEGG	rno:282587.
UCSC	RGD:628691. rat.
Organism-specific databases
CTD	83992.
RGD	628691. Cttnbp2.
Phylogenomic databases
GeneTree	ENSGT00680000099614.
HOGENOM	HOG000112055.
HOVERGEN	HBG073485.
InParanoid	Q2IBD4.
Gene expression databases
ArrayExpress	Q2IBD4.
Genevestigator	Q2IBD4.
Family and domain databases
Gene3D	1.25.40.20. 2 hits.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR019131. Cortactin-binding_p2_N. [Graphical view]
Pfam	PF00023. Ank. 2 hits. PF12796. Ank_2. 1 hit. PF09727. CortBP2. 2 hits. [Graphical view]
SMART	SM00248. ANK. 6 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	624821.

Entry information

Entry name

CTTB2_RAT

Accession

Primary (citable) accession number: Q2IBD4
Secondary accession number(s): O88864

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2013
Last sequence update:	March 7, 2006
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program