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Protein

Alpha-conotoxin Lt14.1

Gene
N/A
Organism
Conus litteratus (Lettered cone)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-conotoxins act on postsynaptic membranes, they bind to the nicotinic acetylcholine receptors (nAChR) and thus inhibit them (PubMed:16797781, PubMed:25617597). This synthetic peptide displays analgesic activity in a hot plate assay (PubMed:16797781, PubMed:25617597, PubMed:21126549). Analgesia is also observed against second phase pain in formalin-induced inflammatory pain model, and in a rat model of mechanically-induced pain (PubMed:25617597). Effects downstream of nAChR are inhibition of calcium influx, inhibition of ERK1/2 phosphorylation and inhibition of c-fos/NOS expression (PubMed:25617597). Genes associated with drug dependence are not up-regulated by this toxin (PubMed:25617597). Treatment with this toxin reversed morphine withdrawal symptoms in mice (PubMed:25617597).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Binds AChR1 Publication1

GO - Molecular functioni

Keywordsi

Molecular functionAcetylcholine receptor inhibiting toxin, Ion channel impairing toxin, Neurotoxin, Postsynaptic neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-conotoxin Lt14.1
Alternative name(s):
Alpha-L-conotoxin Lt14a
OrganismiConus litteratus (Lettered cone)
Taxonomic identifieri89445 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri1153. lt14a variant precursor.
27. LtXIVA precursor.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57K → A: Exhibits higher activity as long-lasting analgesic in the hotplate pain model in mice. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000026266621 – 50By similarityAdd BLAST30
PeptideiPRO_000026266751 – 63Alpha-conotoxin Lt14.1Add BLAST13

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 601 Publication
Disulfide bondi56 ↔ 631 Publication
Modified residuei63Cysteine amide3 Publications1

Post-translational modificationi

May contain a 4-hydroxyproline.Curated

Keywords - PTMi

Amidation, Disulfide bond, Hydroxylation

Expressioni

Tissue specificityi

Expressed by the venom duct.Curated

Structurei

3D structure databases

ProteinModelPortaliQ2I2R5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The cysteine framework is XIV (C-C-C-C).Curated

Sequence similaritiesi

Belongs to the conotoxin L superfamily.Curated

Keywords - Domaini

Signal

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2I2R5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSVMFIVF LMLTMPMTCA GISRSATNGG EADVRAHDKA ANLMALLQER
60
MCPPLCKPSC TNCG
Length:64
Mass (Da):6,878
Last modified:November 28, 2006 - v2
Checksum:i3818FA217399D902
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52C → R in ABC74975 (PubMed:16908117).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ205654 mRNA. Translation: ABA39796.1.
DQ345367 mRNA. Translation: ABC74975.1.

Similar proteinsi

Entry informationi

Entry nameiCLE1_CONLT
AccessioniPrimary (citable) accession number: Q2I2R5
Secondary accession number(s): Q0P0L5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: November 22, 2017
This is version 34 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families