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Protein

Leucine-rich repeat-containing protein 26

Gene

LRRC26

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Auxiliary protein of the large-conductance, voltage and calcium-activated potassium channel (BK alpha). Required for the conversion of BK alpha channels from a high-voltage to a low-voltage activated channel type in non-excitable cells. These are characterized by negative membrane voltages and constant low levels of calcium.2 Publications

GO - Molecular functioni

  • potassium channel regulator activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat-containing protein 26
Alternative name(s):
BK channel auxiliary gamma subunit LRRC26
Cytokeratin-associated protein in cancer
Gene namesi
Name:LRRC26
Synonyms:CAPC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

HGNCiHGNC:31409. LRRC26.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 261235ExtracellularSequence analysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence analysisAdd
BLAST
Topological domaini283 – 33452CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • voltage-gated potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134952867.

Polymorphism and mutation databases

BioMutaiLRRC26.
DMDMi160410009.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Add
BLAST
Chaini27 – 334308Leucine-rich repeat-containing protein 26PRO_0000309360Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 49Sequence analysis
Disulfide bondi47 ↔ 57Sequence analysis
Glycosylationi147 – 1471N-linked (GlcNAc...)1 Publication
Disulfide bondi205 ↔ 231Sequence analysis
Disulfide bondi207 ↔ 253Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ2I0M4.
PaxDbiQ2I0M4.
PRIDEiQ2I0M4.

PTM databases

PhosphoSiteiQ2I0M4.

Expressioni

Tissue specificityi

Isoform 1 is expressed highly in normal prostate and salivary gland, very weakly in colon, pancreas, and intestine, and not at all in other tissues. Isoform 1 is expressed highly in many cancer cell lines and in breast cancer, pancreatic cancer and colon cancer. Isoform 2 is expressed in cancer cell lines.2 Publications

Gene expression databases

BgeeiQ2I0M4.
CleanExiHS_LRRC26.

Organism-specific databases

HPAiHPA056312.

Interactioni

Subunit structurei

Interacts with KCNMA1.2 Publications

Protein-protein interaction databases

DIPiDIP-60459N.
STRINGi9606.ENSP00000360597.

Structurei

3D structure databases

ProteinModelPortaliQ2I0M4.
SMRiQ2I0M4. Positions 43-237.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 7138LRRNTAdd
BLAST
Repeati72 – 9322LRR 1Add
BLAST
Repeati96 – 11722LRR 2Add
BLAST
Repeati120 – 14122LRR 3Add
BLAST
Repeati144 – 16724LRR 4Add
BLAST
Repeati168 – 19023LRR 5Add
BLAST
Domaini201 – 25555LRRCTAdd
BLAST

Domaini

The transmembrane domain is necessary for interaction with KCNMA1.

Sequence similaritiesi

Contains 5 LRR (leucine-rich) repeats.Curated
Contains 1 LRRCT domain.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129749.
HOGENOMiHOG000113367.
HOVERGENiHBG096585.
InParanoidiQ2I0M4.
OMAiDAAFSHC.
OrthoDBiEOG7QZGC2.
PhylomeDBiQ2I0M4.
TreeFamiTF334689.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
[Graphical view]
SMARTiSM00369. LRR_TYP. 5 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2I0M4-1) [UniParc]FASTAAdd to basket

Also known as: L-CAPC

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGPSWSRPR PLLLLLLLLS PWPVWAQVSA TASPSGSLGA PDCPEVCTCV
60 70 80 90 100
PGGLASCSAL SLPAVPPGLS LRLRALLLDH NRVRALPPGA FAGAGALQRL
110 120 130 140 150
DLRENGLHSV HVRAFWGLGA LQLLDLSANQ LEALAPGTFA PLRALRNLSL
160 170 180 190 200
AGNRLARLEP AALGALPLLR SLSLQDNELA ALAPGLLGRL PALDALHLRG
210 220 230 240 250
NPWGCGCALR PLCAWLRRHP LPASEAETVL CVWPGRLTLS PLTAFSDAAF
260 270 280 290 300
SHCAQPLALR DLAVVYTLGP ASFLVSLASC LALGSGLTAC RARRRRLRTA
310 320 330
ALRPPRPPDP NPDPDPHGCA SPADPGSPAA AAQA
Length:334
Mass (Da):34,857
Last modified:November 13, 2007 - v2
Checksum:iB3E8C6C9AADEF958
GO
Isoform 2 (identifier: Q2I0M4-2) [UniParc]FASTAAdd to basket

Also known as: S-CAPC

The sequence of this isoform differs from the canonical sequence as follows:
     1-289: Missing.
     290-290: C → M

Note: Translation initiates from a UGC codon. It is unsure whether the initiator amino acid is a modified cysteine or a methionine. Could also be the result of a proteolytic cleavage from a longer precursor.
Show »
Length:45
Mass (Da):4,736
Checksum:iD7AC871D6CE8057F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271Q → H in ABC79623 (PubMed:16585525).Curated
Sequence conflicti27 – 271Q → H in AAI40912 (PubMed:15164053).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 289289Missing in isoform 2. 1 PublicationVSP_040058Add
BLAST
Alternative sequencei290 – 2901C → M in isoform 2. 1 PublicationVSP_040205

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ355157 mRNA. Translation: ABC79623.1.
EU588721 mRNA. Translation: ACO90295.1.
AL929554 Genomic DNA. Translation: CAH72880.1.
BC140911 mRNA. Translation: AAI40912.1.
CCDSiCCDS35184.1. [Q2I0M4-1]
RefSeqiNP_001013675.1. NM_001013653.2. [Q2I0M4-1]
UniGeneiHs.669977.

Genome annotation databases

EnsembliENST00000371542; ENSP00000360597; ENSG00000184709. [Q2I0M4-1]
GeneIDi389816.
KEGGihsa:389816.
UCSCiuc004clp.4. human. [Q2I0M4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ355157 mRNA. Translation: ABC79623.1.
EU588721 mRNA. Translation: ACO90295.1.
AL929554 Genomic DNA. Translation: CAH72880.1.
BC140911 mRNA. Translation: AAI40912.1.
CCDSiCCDS35184.1. [Q2I0M4-1]
RefSeqiNP_001013675.1. NM_001013653.2. [Q2I0M4-1]
UniGeneiHs.669977.

3D structure databases

ProteinModelPortaliQ2I0M4.
SMRiQ2I0M4. Positions 43-237.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60459N.
STRINGi9606.ENSP00000360597.

PTM databases

PhosphoSiteiQ2I0M4.

Polymorphism and mutation databases

BioMutaiLRRC26.
DMDMi160410009.

Proteomic databases

MaxQBiQ2I0M4.
PaxDbiQ2I0M4.
PRIDEiQ2I0M4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371542; ENSP00000360597; ENSG00000184709. [Q2I0M4-1]
GeneIDi389816.
KEGGihsa:389816.
UCSCiuc004clp.4. human. [Q2I0M4-1]

Organism-specific databases

CTDi389816.
GeneCardsiLRRC26.
H-InvDBHIX0035220.
HGNCiHGNC:31409. LRRC26.
HPAiHPA056312.
MIMi613505. gene.
neXtProtiNX_Q2I0M4.
PharmGKBiPA134952867.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00840000129749.
HOGENOMiHOG000113367.
HOVERGENiHBG096585.
InParanoidiQ2I0M4.
OMAiDAAFSHC.
OrthoDBiEOG7QZGC2.
PhylomeDBiQ2I0M4.
TreeFamiTF334689.

Miscellaneous databases

ChiTaRSiLRRC26. human.
GenomeRNAii389816.
NextBioi103112.
PROiQ2I0M4.
SOURCEiSearch...

Gene expression databases

BgeeiQ2I0M4.
CleanExiHS_LRRC26.

Family and domain databases

Gene3Di3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
[Graphical view]
SMARTiSM00369. LRR_TYP. 5 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Mammary gland and Prostatic carcinoma.
  2. "Non-AUG translational initiation of a short CAPC transcript generating protein isoform."
    Anaganti S., Hansen J.K., Ha D., Hahn Y., Chertov O., Pastan I., Bera T.K.
    Biochem. Biophys. Res. Commun. 380:508-513(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PROTEIN SEQUENCE OF 291-304, USE OF A NON-AUG INITIATOR START CODON, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
  3. "DNA sequence and analysis of human chromosome 9."
    Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L.
    , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
    Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "LRRC26 auxiliary protein allows BK channel activation at resting voltage without calcium."
    Yan J., Aldrich R.W.
    Nature 466:513-516(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KCNMA1, IDENTIFICATION BY MASS SPECTROMETRY.
  6. "BK potassium channel modulation by leucine-rich repeat-containing proteins."
    Yan J., Aldrich R.W.
    Proc. Natl. Acad. Sci. U.S.A. 109:7917-7922(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SIGNAL SEQUENCE, DISULFIDE BONDS, GLYCOSYLATION AT ASN-147, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiLRC26_HUMAN
AccessioniPrimary (citable) accession number: Q2I0M4
Secondary accession number(s): B9EIR7, C3RUL3, Q5VSG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: May 11, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.