Reviewed, UniProtKB/Swiss-Prot Q2HZ26 (TPH2_MACMU)

Names and origin

Protein names

Recommended name:
    Tryptophan 5-hydroxylase 2
    EC=1.14.16.4
Alternative name(s):
    Tryptophan 5-monooxygenase 2

Gene names

Name:

TPH2

Organism

Macaca mulatta (Rhesus macaque)

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.
Cofactor	Fe2+ ion By similarity.
Pathway	Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. Contains 1 ACT domain.

Ontologies

Keywords
Biological process	Serotonin biosynthesis
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	aromatic amino acid family metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW serotonin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 5-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Tryptophan 5-hydroxylase 2		PRO_0000252111
Regions
	Domain	64 – 136	73	ACT
Sites
	Metal binding	318	1	Iron By similarity
	Metal binding	323	1	Iron By similarity
	Metal binding	363	1	Iron By similarity
Amino acid modifications
	Modified residue	382	1	Phosphoserine By similarity
	Modified residue	392	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2HZ26-1 [UniParc]. Last modified March 7, 2006. Version 1. Checksum: DB6A60DD77B4D5AA	FASTA	490	56,095
	10 20 30 40 50 60 MQPAMMMFSS KYWARRGFSL DSAVPEEHQL LGNLTLNKAN SGKNDDKGNK GSSKNETATE 70 80 90 100 110 120 SGKTAVVFSL KNEVGGLVKA LRLFQEKRVH MVHIESRKSR RRSSEVEIFV DCECGKTEFN 130 140 150 160 170 180 ELIQLLKFQT TIVTLNPPEN IWTEEEELED VPWFPRKISE LDKCSHRVLM YGSELDADHP 190 200 210 220 230 240 GFKDNVYRQR RKYFVDVAMG YKYGQPIPRV EYTEEETKTW GVVFRELSKL YPTHACREYL 250 260 270 280 290 300 KNFPLLTKYC GYREDNVPQL EDVSMFLKER SGFTVRPVAG YLSPRDFLAG LAYRVFHCTQ 310 320 330 340 350 360 YIRHGSDPLY TPEPDTCHEL LGHVPLLADP KFAQFSQEIG LASLGASDED VQKLATCYFF 370 380 390 400 410 420 TIEFGLCKQE GQLRAYGAGL LSSIGELKHA LSDKACVKAF DPKTTCLQEC LITTFQEAYF 430 440 450 460 470 480 VSESFEEAKE KMRDFAKSIT RPFSVYFNPY TQSIEILKDT RSIENVVQDL RSDLNTVCDA 490 LNKMNQYLGI

References

[1]	Chen G.-L., Miller G.M. Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ360113 mRNA. Translation: ABC94730.1.
RefSeq	NP_001035035.1.
UniGene	Mmu.10250
3D structure databases
SMR	Q2HZ26. Positions 60-128, 151-484.
ModBase	Search...
Protein-protein interaction databases
STRING	Q2HZ26.
Genome annotation databases
Ensembl	ENSMMUT00000010821; ENSMMUP00000010151; ENSMMUG00000007730; Macaca mulatta. [Genome view]
GeneID	664730.
KEGG	mcc:664730.
Organism-specific databases
CTD	664730.
Phylogenomic databases
eggNOG	prNOG15939.
HOVERGEN	Q2HZ26.
InParanoid	Q2HZ26.
Enzyme and pathway databases
BRENDA	1.14.16.4. 1774.
Family and domain databases
InterPro	IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005963. Trp_5_mOase. IPR019773. Tyrosine_3-monooxygenase-like. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF00351. Biopterin_H. 1 hit. [Graphical view]
PIRSF	PIRSF000336. TH. 1 hit.
PRINTS	PR00372. FYWHYDRXLASE.
TIGRFAMs	TIGR01270. Trp_5_monoox. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPH2_MACMU

Accession

Primary (citable) accession number: Q2HZ26

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	March 7, 2006
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)