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Protein

C-type lectin domain family 12 member B

Gene

CLEC12B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface receptor that protects target cells against natural killer cell-mediated lysis. Modulates signaling cascades and mediates tyrosine phosphorylation of target MAP kinases.1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
C-type lectin domain family 12 member B
Alternative name(s):
Macrophage antigen H
Gene namesi
Name:CLEC12B
ORF Names:UNQ5782/PRO16089
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:31966. CLEC12B.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 4343CytoplasmicSequence analysisAdd
BLAST
Transmembranei44 – 6421Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini65 – 276212ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi7 – 71Y → F: Abolishes tyrosine phosphorylation. Abolishes interaction with PTPN6 and PTPN11. Abolishes protection against natural killer cell-mediated cytotoxicity. 1 Publication

Organism-specific databases

PharmGKBiPA162382325.

Polymorphism and mutation databases

BioMutaiCLEC12B.
DMDMi121941174.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276C-type lectin domain family 12 member BPRO_0000313581Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei7 – 71Phosphotyrosine1 Publication
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence analysis
Disulfide bondi172 ↔ 263PROSITE-ProRule annotation
Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence analysis
Glycosylationi237 – 2371N-linked (GlcNAc...)Sequence analysis
Disulfide bondi242 ↔ 255PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ2HXU8.
PeptideAtlasiQ2HXU8.
PRIDEiQ2HXU8.

PTM databases

iPTMnetiQ2HXU8.
PhosphoSiteiQ2HXU8.

Expressioni

Tissue specificityi

Detected in colon, heart, kidney, liver, lung, mammary gland, ovary, spleen and testis.1 Publication

Gene expression databases

BgeeiQ2HXU8.
CleanExiHS_CLEC12B.
ExpressionAtlasiQ2HXU8. baseline and differential.
GenevisibleiQ2HXU8. HS.

Organism-specific databases

HPAiHPA026973.

Interactioni

Subunit structurei

Homodimer. Interacts with PTPN6 and PTPN11.1 Publication

Protein-protein interaction databases

STRINGi9606.ENSP00000344563.

Structurei

3D structure databases

ProteinModelPortaliQ2HXU8.
SMRiQ2HXU8. Positions 110-268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini150 – 264115C-type lectinPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi5 – 106ITIM motif

Domaini

Sequence similaritiesi

Contains 1 C-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00700000104266.
HOGENOMiHOG000111777.
HOVERGENiHBG097789.
InParanoidiQ2HXU8.
KOiK17517.
OMAiLCQELII.
OrthoDBiEOG725DJD.
PhylomeDBiQ2HXU8.
TreeFamiTF336674.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR013600. Ly49_N.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF08391. Ly49. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2HXU8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSEEVTYATL TFQDSAGARN NRDGNNLRKR GHPAPSPIWR HAALGLVTLC
60 70 80 90 100
LMLLIGLVTL GMMFLQISND INSDSEKLSQ LQKTIQQQQD NLSQQLGNSN
110 120 130 140 150
NLSMEEEFLK SQISSVLKRQ EQMAIKLCQE LIIHTSDHRC NPCPKMWQWY
160 170 180 190 200
QNSCYYFTTN EEKTWANSRK DCIDKNSTLV KIDSLEEKDF LMSQPLLMFS
210 220 230 240 250
FFWLGLSWDS SGRSWFWEDG SVPSPSLFST KELDQINGSK GCAYFQKGNI
260 270
YISRCSAEIF WICEKTAAPV KTEDLD
Length:276
Mass (Da):31,616
Last modified:March 7, 2006 - v1
Checksum:i3FD95A30C5C1BAC4
GO
Isoform 2 (identifier: Q2HXU8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     228-232: FSTKE → YVSNY
     233-276: Missing.

Show »
Length:232
Mass (Da):26,740
Checksum:i3435E7021D177406
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti6 – 61T → N.1 Publication
Corresponds to variant rs1359082 [ dbSNP | Ensembl ].
VAR_037670
Natural varianti116 – 1161V → L.2 Publications
Corresponds to variant rs637790 [ dbSNP | Ensembl ].
VAR_037671

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei228 – 2325FSTKE → YVSNY in isoform 2. 2 PublicationsVSP_030034
Alternative sequencei233 – 27644Missing in isoform 2. 2 PublicationsVSP_030035Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ368812 mRNA. Translation: ABC96323.1.
AY358810 mRNA. Translation: AAQ89170.1.
AK128243 mRNA. Translation: BAC87350.1.
CH471094 Genomic DNA. Translation: EAW96136.1.
CCDSiCCDS44830.1. [Q2HXU8-1]
CCDS8610.1. [Q2HXU8-2]
RefSeqiNP_001123470.1. NM_001129998.2. [Q2HXU8-1]
NP_995324.2. NM_205852.3. [Q2HXU8-2]
XP_006719134.1. XM_006719071.2. [Q2HXU8-2]
XP_006719135.1. XM_006719072.1. [Q2HXU8-2]
UniGeneiHs.127937.

Genome annotation databases

EnsembliENST00000338896; ENSP00000344563; ENSG00000256660. [Q2HXU8-1]
ENST00000396502; ENSP00000379759; ENSG00000256660. [Q2HXU8-2]
ENST00000544853; ENSP00000439561; ENSG00000256660. [Q2HXU8-2]
GeneIDi387837.
KEGGihsa:387837.
UCSCiuc001qwx.3. human. [Q2HXU8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ368812 mRNA. Translation: ABC96323.1.
AY358810 mRNA. Translation: AAQ89170.1.
AK128243 mRNA. Translation: BAC87350.1.
CH471094 Genomic DNA. Translation: EAW96136.1.
CCDSiCCDS44830.1. [Q2HXU8-1]
CCDS8610.1. [Q2HXU8-2]
RefSeqiNP_001123470.1. NM_001129998.2. [Q2HXU8-1]
NP_995324.2. NM_205852.3. [Q2HXU8-2]
XP_006719134.1. XM_006719071.2. [Q2HXU8-2]
XP_006719135.1. XM_006719072.1. [Q2HXU8-2]
UniGeneiHs.127937.

3D structure databases

ProteinModelPortaliQ2HXU8.
SMRiQ2HXU8. Positions 110-268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000344563.

PTM databases

iPTMnetiQ2HXU8.
PhosphoSiteiQ2HXU8.

Polymorphism and mutation databases

BioMutaiCLEC12B.
DMDMi121941174.

Proteomic databases

PaxDbiQ2HXU8.
PeptideAtlasiQ2HXU8.
PRIDEiQ2HXU8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338896; ENSP00000344563; ENSG00000256660. [Q2HXU8-1]
ENST00000396502; ENSP00000379759; ENSG00000256660. [Q2HXU8-2]
ENST00000544853; ENSP00000439561; ENSG00000256660. [Q2HXU8-2]
GeneIDi387837.
KEGGihsa:387837.
UCSCiuc001qwx.3. human. [Q2HXU8-1]

Organism-specific databases

CTDi387837.
GeneCardsiCLEC12B.
HGNCiHGNC:31966. CLEC12B.
HPAiHPA026973.
neXtProtiNX_Q2HXU8.
PharmGKBiPA162382325.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4297. Eukaryota.
ENOG410XPJ1. LUCA.
GeneTreeiENSGT00700000104266.
HOGENOMiHOG000111777.
HOVERGENiHBG097789.
InParanoidiQ2HXU8.
KOiK17517.
OMAiLCQELII.
OrthoDBiEOG725DJD.
PhylomeDBiQ2HXU8.
TreeFamiTF336674.

Miscellaneous databases

ChiTaRSiCLEC12B. human.
GenomeRNAii387837.
PROiQ2HXU8.

Gene expression databases

BgeeiQ2HXU8.
CleanExiHS_CLEC12B.
ExpressionAtlasiQ2HXU8. baseline and differential.
GenevisibleiQ2HXU8. HS.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR016187. C-type_lectin_fold.
IPR013600. Ly49_N.
[Graphical view]
PfamiPF00059. Lectin_C. 1 hit.
PF08391. Ly49. 1 hit.
[Graphical view]
SMARTiSM00034. CLECT. 1 hit.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
PROSITEiPS50041. C_TYPE_LECTIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, INTERACTION WITH PTPN6 AND PTPN11, TISSUE SPECIFICITY, MUTAGENESIS OF TYR-7, PHOSPHORYLATION AT TYR-7, ALTERNATIVE SPLICING.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT LEU-116.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS ASN-6 AND LEU-116.
    Tissue: Testis.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiCL12B_HUMAN
AccessioniPrimary (citable) accession number: Q2HXU8
Secondary accession number(s): Q6UWF2, Q6ZRG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: March 7, 2006
Last modified: July 6, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.