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Protein

ER degradation-enhancing alpha-mannosidase-like protein 3

Gene

Edem3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes, by catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2 in the N-glycans. Seems to have alpha 1,2-mannosidase activity.By similarity1 Publication

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei147Proton donorBy similarity1
Active sitei294By similarity1
Active sitei388Proton donorBy similarity1
Active sitei406By similarity1
Metal bindingi492CalciumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processUnfolded protein response
LigandMetal-binding

Enzyme and pathway databases

ReactomeiR-MMU-901032 ER Quality Control Compartment (ERQC)
UniPathwayiUPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
ER degradation-enhancing alpha-mannosidase-like protein 3 (EC:3.2.1.113By similarity)
Alternative name(s):
Alpha-1,2-mannosidase EDEM3
Gene namesi
Name:Edem3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1914217 Edem3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi147E → Q: Loss of ERAD activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 42Sequence analysisAdd BLAST42
ChainiPRO_000031695843 – 931ER degradation-enhancing alpha-mannosidase-like protein 3Add BLAST889

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi119N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi196N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi505N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi512N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi811N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi815N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi899N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ2HXL6
MaxQBiQ2HXL6
PaxDbiQ2HXL6
PeptideAtlasiQ2HXL6
PRIDEiQ2HXL6

PTM databases

iPTMnetiQ2HXL6
PhosphoSitePlusiQ2HXL6

Expressioni

Tissue specificityi

Widely expressed. Expressed at higher level in liver, heart and kidney.1 Publication

Inductioni

Slightly increased by endoplasmic reticulum stress.1 Publication

Gene expression databases

BgeeiENSMUSG00000043019
CleanExiMM_EDEM3
ExpressionAtlasiQ2HXL6 baseline and differential
GenevisibleiQ2HXL6 MM

Interactioni

Protein-protein interaction databases

IntActiQ2HXL6, 6 interactors
STRINGi10090.ENSMUSP00000058941

Structurei

3D structure databases

ProteinModelPortaliQ2HXL6
SMRiQ2HXL6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini675 – 780PAAdd BLAST106

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi928 – 931Prevents secretion from ERPROSITE-ProRule annotation4

Domaini

Contains a protease-associated domain of unknown function.

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2429 Eukaryota
ENOG410XS6F LUCA
GeneTreeiENSGT00530000063069
HOGENOMiHOG000007735
HOVERGENiHBG107835
InParanoidiQ2HXL6
KOiK10086
PhylomeDBiQ2HXL6
TreeFamiTF300807

Family and domain databases

CDDicd02126 PA_EDEM3_like, 1 hit
Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR037322 EDEM3_PA
IPR001382 Glyco_hydro_47
IPR003137 PA_domain
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PF02225 PA, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2HXL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKAGGCRGC GCRVPQRASW SLVAATAALC LVLATSVCTA GAAPMSREEK
60 70 80 90 100
QKLGNQVLEM FDHAYGNYME HAYPADELMP LTCRGRVRGQ EPSRGDVDDA
110 120 130 140 150
LGKFSLTLID SLDTLVVLNK TKEFEDAVRK VLRDVNLDND VVVSVFETNI
160 170 180 190 200
RVLGGLLGGH SLAIMLKEKG EHMQWYNDEL LHMAKQLGYK LLPAFNTTSG
210 220 230 240 250
LPYPRINLKF GIRKPEARTG TETDTCTACA GTLILEFAAL SRFTGATIFE
260 270 280 290 300
EYARKALDFL WEKRQRSSNL VGVTINIHTG DWVRKDSGVG AGIDSYYEYL
310 320 330 340 350
LKAYVLLGDD SFLERFNTHY DAIMRYISQP PLLLDVHIHK PMLNARTWMD
360 370 380 390 400
ALLAFFPGLQ VLKGDIRPAI ETHEMLYQVI KKHNFLPEAF TTDFRVHWAQ
410 420 430 440 450
HPLRPEFAES TYFLYKATGD PYYLEVGKTL IENLNKYARV PCGFAAMKDV
460 470 480 490 500
RTGSHEDRMD SFFLAEMFKY LYLLFADKED IIFDIEDYIF TTEAHLLPLW
510 520 530 540 550
LSTTNRSISK KNTTSEYTEL DDSNFDWTCP NTQILFPNDP LYAQSIREPL
560 570 580 590 600
KNVVDKSCPR GIIRVEESFR SGAKPPLRAR DFMATNPEHL EILKKMGVSL
610 620 630 640 650
IHLKDGRVQL VQHAIQAASS IDAEDGLRFM QEMIELSSQQ QKEQQLPPRA
660 670 680 690 700
VQIISHPFFG RVVLTAGPAQ FGLDLSKHKE TRGFVASSKP YNGCSELTNP
710 720 730 740 750
EAVMGKIALI QRGQCMFAEK ARNIQNAGAI GGIVIDDNEG SSSDTAPLFQ
760 770 780 790 800
MAGDGKDTDD IKIPMLFLFS KEGSIILDAI REHEQVEVLL SDKARDRDPE
810 820 830 840 850
MENEDQPSSE NDSQNQSAEQ MLSLSQTVDL ADKESPEHPA DSHSEASPSD
860 870 880 890 900
SEEAAGFAPS EQISGSTENH ETTSLDGECT DLDNQVQEQS ETEEDSSPNV
910 920 930
SWGTKAQPID SILADWNEDI EAFEMMEKDE L
Length:931
Mass (Da):104,200
Last modified:March 3, 2009 - v2
Checksum:i4F010D17E84E542C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti784E → K in BAE79485 (PubMed:16431915).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB188342 mRNA Translation: BAE79485.1
BC060718 mRNA Translation: AAH60718.1
BC138658 mRNA Translation: AAI38659.1
BC138659 mRNA Translation: AAI38660.1
CCDSiCCDS35736.1
RefSeqiNP_001034733.2, NM_001039644.2
UniGeneiMm.337691

Genome annotation databases

EnsembliENSMUST00000059498; ENSMUSP00000058941; ENSMUSG00000043019
GeneIDi66967
KEGGimmu:66967
UCSCiuc007czc.2 mouse

Similar proteinsi

Entry informationi

Entry nameiEDEM3_MOUSE
AccessioniPrimary (citable) accession number: Q2HXL6
Secondary accession number(s): B2RS10, B9EHZ4, Q6P9L0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: March 3, 2009
Last modified: March 28, 2018
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health