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Protein

Respiratory burst oxidase homolog protein D

Gene

RBOHD

Organism
Solanum tuberosum (Potato)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide. May be responsible for the oxidative burst in response to pathogen attack in the leaves.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi207CalciumBy similarity1
Metal bindingi209CalciumBy similarity1
Metal bindingi211CalciumBy similarity1
Metal bindingi213Calcium; via carbonyl oxygenBy similarity1
Metal bindingi218CalciumBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi207 – 218PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase, Peroxidase
LigandCalcium, FAD, Flavoprotein, Metal-binding, NADP

Protein family/group databases

PeroxiBasei4548. StRboh04.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein D (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
NADPH oxidase RBOHD
StRBOHD
Gene namesi
Name:RBOHD
OrganismiSolanum tuberosum (Potato)
Taxonomic identifieri4113 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanum
Proteomesi
  • UP000011115 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 318CytoplasmicSequence analysisAdd BLAST318
Transmembranei319 – 339HelicalSequence analysisAdd BLAST21
Topological domaini340 – 351ExtracellularSequence analysisAdd BLAST12
Transmembranei352 – 372HelicalSequence analysisAdd BLAST21
Topological domaini373 – 397CytoplasmicSequence analysisAdd BLAST25
Transmembranei398 – 418HelicalSequence analysisAdd BLAST21
Topological domaini419 – 454ExtracellularSequence analysisAdd BLAST36
Transmembranei455 – 475HelicalSequence analysisAdd BLAST21
Topological domaini476 – 497CytoplasmicSequence analysisAdd BLAST22
Transmembranei498 – 518HelicalSequence analysisAdd BLAST21
Topological domaini519 – 675ExtracellularSequence analysisAdd BLAST157
Transmembranei676 – 696HelicalSequence analysisAdd BLAST21
Topological domaini697 – 858CytoplasmicSequence analysisAdd BLAST162

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003137661 – 858Respiratory burst oxidase homolog protein DAdd BLAST858

Post-translational modificationi

Phosphorylated by CPK.1 Publication

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Expressed in leaves.1 Publication

Interactioni

Subunit structurei

Monomer and homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ2HXK9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini194 – 229EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini238 – 273EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini357 – 514Ferric oxidoreductaseAdd BLAST158
Domaini548 – 670FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni134 – 144EF-hand-like 1By similarityAdd BLAST11
Regioni171 – 182EF-hand-like 2By similarityAdd BLAST12

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiQ2HXK9.
KOiK13447.

Family and domain databases

CDDicd00051. EFh. 1 hit.
InterProiView protein in InterPro
IPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2HXK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNPEDHHSD RELSSPSNTT KSNDDKNVEI TLDIRDDTMA GQSVKNATKT
60 70 80 90 100
KAEEAELEAL GKNLQKKCSF GATIVRNVSM RMRLPSFKRQ PHPPQTFDRS
110 120 130 140 150
STAAQNALKG FKFISKTDGG SGWDTVQQRF DELTATSDSL LPRAKFGECI
160 170 180 190 200
GMNRESEGFA LELFNALARR RNITSGCISK EQLKEFWDQI ANQSFDSRLR
210 220 230 240 250
TFFDMVDKDA DGRLTEEEVR EIICLSASAN KLSNIQKQAA EYAALIMEEL
260 270 280 290 300
DRDQKGYIML ENLEMLLLEA PIQPDGEKGL NRNLSHMLSM KLKPTLETNP
310 320 330 340 350
IKRWYNNLKY FLLDNWRRVW VLLLWIGVMA GLFAYKYVQY KNKAAFNVMG
360 370 380 390 400
HCVCVAKGAA EVLKLNMALI LLPVCRNTIT WLRNKTKLGG AVPFDDNINF
410 420 430 440 450
HKVVAGAIAV GVGIHVLAHM TCDFPRLLNA SPEKYKPMEP YFGDQPRNYW
460 470 480 490 500
HFVKGVEGVS GIIMVVLMSI AFTLASQRFR RNKIRLPRPL NKLTGFNAFW
510 520 530 540 550
YSHHLFVIVY SLLIVHGIEL YLTKEWYKKT TWMYLAIPII LYSGERLLRA
560 570 580 590 600
FRSSVKDVKI LKVAMYTGNV LTLQMSKPQG FNYKSGQYMF VNCAAVSPFE
610 620 630 640 650
WHPFSITSAP GDEYLSVHIR IVGDWTTKLR DVFSEPSPTG RSGLVADYLQ
660 670 680 690 700
DKINYPKVLI DGPYGAPAQD YKEYEVLLLV GLGIGATPMI SIVKDIVNNM
710 720 730 740 750
KEEKYDHDLE KKTVSGSGRS NFKRVYFYWV TREQGSFDWF KGLMNELAVM
760 770 780 790 800
DCDGIIEMHN YCTSVYEEGD ARSALIAMLQ SINHAKNGVD IVSGTRVKTH
810 820 830 840 850
FARPNWRNVY KRIALNHTDA RVGVFYCGAP ALTKVLGQLA LDFSHKTSTK

FDFHKENF
Length:858
Mass (Da):97,539
Last modified:June 12, 2007 - v2
Checksum:i9414DDA428D6EBB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB198717 mRNA. Translation: BAE79345.2.
RefSeqiNP_001305507.1. NM_001318578.1.
UniGeneiStu.17713.

Genome annotation databases

GeneIDi102586476.
KEGGisot:102586476.

Similar proteinsi

Entry informationi

Entry nameiRBOHD_SOLTU
AccessioniPrimary (citable) accession number: Q2HXK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 12, 2007
Last modified: May 10, 2017
This is version 74 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families