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Q2HWU2

- PDIA1_MACFU

UniProt

Q2HWU2 - PDIA1_MACFU

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Protein

Protein disulfide-isomerase

Gene
P4HB, PDI, PDIA1
Organism
Macaca fuscata fuscata (Japanese macaque)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP By similarity.

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei55 – 551Nucleophile By similarity
Sitei56 – 561Contributes to redox potential value By similarity
Sitei57 – 571Contributes to redox potential value By similarity
Active sitei58 – 581Nucleophile By similarity
Sitei122 – 1221Lowers pKa of C-terminal Cys of first active site By similarity
Active sitei399 – 3991Nucleophile By similarity
Sitei400 – 4001Contributes to redox potential value By similarity
Sitei401 – 4011Contributes to redox potential value By similarity
Active sitei402 – 4021Nucleophile By similarity
Sitei463 – 4631Lowers pKa of C-terminal Cys of second active site By similarity

GO - Molecular functioni

  1. protein disulfide isomerase activity Source: UniProtKB-EC

GO - Biological processi

  1. cell redox homeostasis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase (EC:5.3.4.1)
Short name:
PDI
Alternative name(s):
Prolyl 4-hydroxylase subunit beta
Gene namesi
Name:P4HB
Synonyms:PDI, PDIA1
OrganismiMacaca fuscata fuscata (Japanese macaque)
Taxonomic identifieri9543 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniCercopithecidaeCercopithecinaeMacaca

Subcellular locationi

Endoplasmic reticulum lumen By similarity. Melanosome By similarity. Cell membrane; Peripheral membrane protein Reviewed prediction
Note: Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. Localizes near CD4-enriched regions on lymphoid cell surfaces By similarity.

GO - Cellular componenti

  1. endoplasmic reticulum Source: MGI
  2. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  3. melanosome Source: UniProtKB-SubCell
  4. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 By similarityAdd
BLAST
Chaini20 – 510491Protein disulfide-isomerasePRO_0000246155Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi55 ↔ 58Redox-active By similarity
Modified residuei202 – 2021N6-acetyllysine By similarity
Modified residuei224 – 2241N6-succinyllysine By similarity
Modified residuei273 – 2731N6-succinyllysine By similarity
Disulfide bondi399 ↔ 402Redox-active By similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PRIDEiQ2HWU2.

Interactioni

Subunit structurei

Homodimer. Monomers and homotetramers may also occur. Also constitutes the structural subunit of prolyl 4-hydroxylase and of the microsomal triacylglycerol transfer protein MTTP in mammalian cells. Stabilizes both enzymes and retain them in the ER without contributing to the catalytic activity. Binds UBQLN1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ2HWU2.
SMRiQ2HWU2. Positions 20-359, 370-473.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 136117Thioredoxin 1Add
BLAST
Domaini351 – 477127Thioredoxin 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi507 – 5104Prevents secretion from ER By similarity

Sequence similaritiesi

Contains 2 thioredoxin domains.

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

HOVERGENiHBG005920.

Family and domain databases

Gene3Di3.40.30.10. 4 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 4 hits.
TIGRFAMsiTIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2HWU2-1 [UniParc]FASTAAdd to Basket

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MLRRALLCLA VAAAPGLYAD APEEEDHVLV LRKSNFAEAL AAHKYLLVEF    50
YAPWCGHCKA LAPEYAKAAG KLKAEGSEIR LAKVDATEES DLAQQYGVRG 100
YPTIKFFRNG DTASPKEYTA GREADDIVNW LKKRTGPAAT TLPDGAAAES 150
LVESSEVAVI GFFKDVESDS AKQFLQAAEA IDDIPFGITS NSDVFSKYQL 200
DKDGVVLFKK FDEGRNNFEG EVTKENLLDF IKYNQLPLVI EFTEQTAPKI 250
FGGEIKTHIL LFLPKSVSDY DGKLSNFKTA AESFKGKILF IFIDSDHTDN 300
QRILEFFGLK KEECPAVRLI TLEEEMTKYK PESDELTAER ITEFCHRFLE 350
GKIKPHLMSQ ELPEDWDKQP VKVLVGKNFE EVAFDENKNV FVEFYAPWCG 400
HCKQLAPIWD KLGETYKDHE NIVIAKMDST ANEVEAIKVH SFPTLKFFPA 450
SVDRTVIDYN GERTLDGFKK FLESGGQDGA GDDDDLEDLE EAEEPDMEED 500
DDQKAVKDEL 510
Length:510
Mass (Da):57,304
Last modified:March 21, 2006 - v1
Checksum:i5F917B017F418BE2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB232156 mRNA. Translation: BAE79726.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB232156 mRNA. Translation: BAE79726.1 .

3D structure databases

ProteinModelPortali Q2HWU2.
SMRi Q2HWU2. Positions 20-359, 370-473.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q2HWU2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG005920.

Family and domain databases

Gene3Di 3.40.30.10. 4 hits.
InterProi IPR005788. Disulphide_isomerase.
IPR005792. Prot_disulphide_isomerase.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view ]
Pfami PF00085. Thioredoxin. 2 hits.
[Graphical view ]
SUPFAMi SSF52833. SSF52833. 4 hits.
TIGRFAMsi TIGR01130. ER_PDI_fam. 1 hit.
TIGR01126. pdi_dom. 2 hits.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and gene expression of endoplasmic reticulum stress proteins in Japanese monkey, Macaca fuscata."
    Higashino A., Fukuhara R., Tezuka T., Kageyama T.
    J. Med. Primatol. 35:376-383(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPDIA1_MACFU
AccessioniPrimary (citable) accession number: Q2HWU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 21, 2006
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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