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Protein

Formin-binding protein 1-like

Gene

Fnbp1l

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL-WASPIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Essential for autophagy of intracellular bacterial pathogens (By similarity). May negatively regulate neurite extension and axon branching in developing neurons.By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei165Mediates end-to-end attachment of dimersBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Autophagy, Differentiation, Endocytosis, Neurogenesis

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-RNO-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Formin-binding protein 1-like
Alternative name(s):
Transducer of Cdc42-dependent actin assembly protein 1
Short name:
Toca-1
Gene namesi
Name:Fnbp1l
Synonyms:Toca1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi1305386. Fnbp1l.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi51 – 52KK → QQ: Impairs membrane tubulation and suppression of neurite elongation. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002614361 – 605Formin-binding protein 1-likeAdd BLAST605

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295PhosphoserineCombined sources1
Modified residuei488PhosphoserineBy similarity1
Modified residuei501PhosphoserineCombined sources1
Modified residuei505PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2HWF0.
PRIDEiQ2HWF0.

PTM databases

iPTMnetiQ2HWF0.
PhosphoSitePlusiQ2HWF0.

Expressioni

Tissue specificityi

Isoform 1 is expressed in brain. Isoform 2 is expressed in brain, kidney and lung. Within the brain expression is seen in cortical neurons, hippocampal pyramidal neurons, hypothalamus and piriform cortex.1 Publication

Developmental stagei

Expression in brain declines from E18 to P8 and is undetectable from P14 onwards.1 Publication

Gene expression databases

ExpressionAtlasiQ2HWF0. baseline and differential.
GenevisibleiQ2HWF0. RN.

Interactioni

Subunit structurei

Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures. Interacts with GTP-bound CDC42. Interacts with DAAM1, DIAPH1, DIAPH2, DNM1, DNM2 and WASL/N-WASP. Interacts with ATG3. Interacts (via SH3 domain) with ABI1, WASF2, CDC42 and WIPF1.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052531.

Structurei

3D structure databases

ProteinModelPortaliQ2HWF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 263F-BARPROSITE-ProRule annotationAdd BLAST263
Repeati409 – 484REMAdd BLAST76
Domaini538 – 599SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni245 – 535Interaction with CDC42By similarityAdd BLAST291
Regioni522 – 605Interaction with DNM1By similarityAdd BLAST84
Regioni541 – 605Interaction with DAAM1, DIAPH1 and DIAPH2By similarityAdd BLAST65
Regioni541 – 597Interaction with DNM2 and WASLBy similarityAdd BLAST57

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili66 – 258By similarityAdd BLAST193
Coiled coili392 – 484By similarityAdd BLAST93

Domaini

The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation (By similarity).By similarity

Sequence similaritiesi

Belongs to the FNBP1 family.Curated
Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 REM (Hr1) repeat.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiENOG410INNW. Eukaryota.
ENOG410XS31. LUCA.
GeneTreeiENSGT00510000046403.
HOGENOMiHOG000231767.
HOVERGENiHBG002489.
InParanoidiQ2HWF0.
KOiK20121.
OMAiGWSVKMG.
OrthoDBiEOG091G038P.
PhylomeDBiQ2HWF0.
TreeFamiTF351162.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR030116. FNBP1L.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10663:SF154. PTHR10663:SF154. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q2HWF0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSWGTELWDQ FDSLDKHTQW GIDFLERYAK FVKERIEIEQ NYAKQLRNLV
60 70 80 90 100
KKYCPKRSSK DEEPRFTSCI AFFNILNELN DYAGQREVVA EEMAHRVYGE
110 120 130 140 150
LMRYAHDLKT ERKMHLQEGR KAQQYLDMCW KQMDNSKKKF ERECREAEKA
160 170 180 190 200
QQSYERLDND TNATKADVEK AKQQLNLRTH MADENKNEYA AQLQNFNGEQ
210 220 230 240 250
HKHFYVVIPQ IYKQLQEMDE RRTIKLSECY RGFADSERKV IPIISKCLEG
260 270 280 290 300
MILAAKSVDE RRDSQMVVDS FKSGFEPPGD FPFEDYSQHI YRTVSDGTIS
310 320 330 340 350
ASKQEGGKMD SKSTAGKAKG KLWLFGKKPK PQSPPLTPTS LFTSSPPNGS
360 370 380 390 400
QFLTLSIEPV HYCMNEIKTG KPRIPSFRSL KRGWSMKMGP ALEDFSHLPP
410 420 430 440 450
EQRRKKLQQR IDELNRGLQK ESDQKEALNK MKDVYEKNPQ MGDPGSLQPK
460 470 480 490 500
LAETMNNIDR LRMEIHKNEA WLSEVEGKTG VRGDRRHSSD INHLVTQGRE
510 520 530 540 550
SPEGSYTDDA NQEVRGPPQQ HGHHSEFDDE FEDDDPLPAI GHCKAIYPFD
560 570 580 590 600
GHNEGTLAMK EGEVLYIIEE DKGDGWTRAR RQNGEEGYVP TTYIDVTLEK

NSKGS
Note: Gene prediction based on EST data.
Length:605
Mass (Da):69,974
Last modified:November 28, 2006 - v2
Checksum:i6DF6AA35D562FAF7
GO
Isoform 2 (identifier: Q2HWF0-2) [UniParc]FASTAAdd to basket
Also known as: Long, L

The sequence of this isoform differs from the canonical sequence as follows:
     605-605: S → AVTYI

Show »
Length:609
Mass (Da):70,435
Checksum:iC862B6CFC596AA35
GO
Isoform 3 (identifier: Q2HWF0-3) [UniParc]FASTAAdd to basket
Also known as: Short, S

The sequence of this isoform differs from the canonical sequence as follows:
     331-388: Missing.
     605-605: S → AVTYI

Show »
Length:551
Mass (Da):63,947
Checksum:i6B93588F7274B088
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021714331 – 388Missing in isoform 3. 1 PublicationAdd BLAST58
Alternative sequenceiVSP_021715605S → AVTYI in isoform 2 and isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB250295 mRNA. Translation: BAE79635.1.
AB250296 mRNA. Translation: BAE79636.1.
AABR03012297 Genomic DNA. No translation available.
RefSeqiNP_001034698.1. NM_001039609.1. [Q2HWF0-2]
UniGeneiRn.213503.

Genome annotation databases

EnsembliENSRNOT00000019244; ENSRNOP00000019244; ENSRNOG00000013798. [Q2HWF0-3]
ENSRNOT00000055669; ENSRNOP00000052531; ENSRNOG00000013798. [Q2HWF0-1]
GeneIDi310839.
KEGGirno:310839.
UCSCiRGD:1305386. rat. [Q2HWF0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB250295 mRNA. Translation: BAE79635.1.
AB250296 mRNA. Translation: BAE79636.1.
AABR03012297 Genomic DNA. No translation available.
RefSeqiNP_001034698.1. NM_001039609.1. [Q2HWF0-2]
UniGeneiRn.213503.

3D structure databases

ProteinModelPortaliQ2HWF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000052531.

PTM databases

iPTMnetiQ2HWF0.
PhosphoSitePlusiQ2HWF0.

Proteomic databases

PaxDbiQ2HWF0.
PRIDEiQ2HWF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019244; ENSRNOP00000019244; ENSRNOG00000013798. [Q2HWF0-3]
ENSRNOT00000055669; ENSRNOP00000052531; ENSRNOG00000013798. [Q2HWF0-1]
GeneIDi310839.
KEGGirno:310839.
UCSCiRGD:1305386. rat. [Q2HWF0-1]

Organism-specific databases

CTDi54874.
RGDi1305386. Fnbp1l.

Phylogenomic databases

eggNOGiENOG410INNW. Eukaryota.
ENOG410XS31. LUCA.
GeneTreeiENSGT00510000046403.
HOGENOMiHOG000231767.
HOVERGENiHBG002489.
InParanoidiQ2HWF0.
KOiK20121.
OMAiGWSVKMG.
OrthoDBiEOG091G038P.
PhylomeDBiQ2HWF0.
TreeFamiTF351162.

Enzyme and pathway databases

ReactomeiR-RNO-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

PROiQ2HWF0.

Gene expression databases

ExpressionAtlasiQ2HWF0. baseline and differential.
GenevisibleiQ2HWF0. RN.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR030116. FNBP1L.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10663:SF154. PTHR10663:SF154. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFBP1L_RAT
AccessioniPrimary (citable) accession number: Q2HWF0
Secondary accession number(s): Q2HWE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: November 30, 2016
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.