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Protein

Shutoff alkaline exonuclease

Gene

ORF37

Organism
Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in processing non linear or branched viral DNA intermediates in order to promote the production of mature packaged unit-length linear progeny viral DNA molecules. Exhibits endonuclease and exonuclease activities and accepts both double-stranded and single-stranded DNA as substrate. Exonuclease digestion of DNA is in the 5'-> 3' direction and the products are 5'-monophosphate nucleosides. Additionally, forms a recombinase with the major DNA-binding protein, which displays strand exchange activity. Also acts as a cytoplasmic RNA endonuclease that induces degradation of the majority of the cellular messenger RNAs during early lytic infection. The resulting inhibition of cellular protein synthesis serves to ensure maximal viral gene expression and evasion from host immune response. Internally cleaves host mRNAs which are then degraded by the cellular exonuclease XRN1. Bypasses therefore the regulatory steps of deadenylation and decapping normally required for XRN1 activation.UniRule annotation3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei184Required for functionUniRule annotation1
Sitei221Required for functionUniRule annotation1
Sitei244Required for functionUniRule annotation1
Sitei246Required for functionUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease, RNA-binding
Biological processDecay of host mRNAs by virus, Eukaryotic host gene expression shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Shutoff alkaline exonucleaseUniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
SOXUniRule annotation
Gene namesi
Name:ORF37
OrganismiHuman herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Taxonomic identifieri868565 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000000942 Componenti: Genome

Subcellular locationi

Q2HR95:
  • Host nucleus UniRule annotation1 Publication
  • Host cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi184E → A: Complete loss of ability to deplete cellular mRNAs. 1 Publication1
Mutagenesisi221D → A or S: Complete loss of ability to deplete cellular mRNAs. 1 Publication1
Mutagenesisi244E → A: Complete loss of ability to deplete cellular mRNAs. 1 Publication1
Mutagenesisi246K → I: Complete loss of ability to deplete cellular mRNAs. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004231451 – 486Shutoff alkaline exonucleaseAdd BLAST486

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Forms a complex with the DNA polymerase, the DNA polymerase processivity factor, and the major DNA binding protein.UniRule annotation

Structurei

3D structure databases

SMRiQ2HR95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the herpesviridae alkaline nuclease family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900004U.

Family and domain databases

Gene3Di3.90.320.10. 1 hit.
HAMAPiMF_04009. HSV_AN. 1 hit.
InterProiView protein in InterPro
IPR011604. Exonuc_phg/RecB_C.
IPR001616. Herpes_alk_exo.
IPR011335. Restrct_endonuc-II-like.
IPR034720. Viral_alk_exo.
PfamiView protein in Pfam
PF01771. Herpes_alk_exo. 1 hit.
PRINTSiPR00924. ALKEXNUCLASE.
SUPFAMiSSF52980. SSF52980. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2HR95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEATPTPADL FSEDYLVDTL DGLTVDDQQA VLASLSFSKF LKHAKVRDWC
60 70 80 90 100
AQAKIQPSMP ALRMAYNYFL FSKVGEFIGS EDVCNFFVDR VFGGVRLLDV
110 120 130 140 150
ASVYAACSQM NAHQRHHICC LVERATSSQS LNPVWDALRD GIISSSKFHW
160 170 180 190 200
AVKQQNTSKK IFSPWPITNN HFVAGPLAFG LRCEEVVKTL LATLLHPDEA
210 220 230 240 250
NCLDYGFMQS PQNGIFGVSL DFAANVKTDT EGRLQFDPNC KVYEIKCRFK
260 270 280 290 300
YTFAKMECDP IYAAYQRLYE APGKLALKDF FYSISKPAVE YVGLGKLPSE
310 320 330 340 350
SDYLVAYDQE WEACPRKKRK LTPLHNLIRE CILHNSTTES DVYVLTDPQD
360 370 380 390 400
TRGQISIKAR FKANLFVNVR HSYFYQVLLQ SSIVEEYIGL DSGIPRLGSP
410 420 430 440 450
KYYIATGFFR KRGYQDPVNC TIGGDALDPH VEIPTLLIVT PVYFPRGAKH
460 470 480
RLLHQAANFW SRSAKDTFPY IKWDFSYLSA NVPHSP
Length:486
Mass (Da):55,076
Last modified:March 21, 2006 - v1
Checksum:iD7E0FA2FCB04AACF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148805 Genomic DNA. Translation: ABD28888.1.
RefSeqiYP_001129390.1. NC_009333.1.

Genome annotation databases

GeneIDi4961512.

Similar proteinsi

Entry informationi

Entry nameiAN_HHV8P
AccessioniPrimary (citable) accession number: Q2HR95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: March 21, 2006
Last modified: September 27, 2017
This is version 55 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families