Q2HR75 (ORF57_HHV8P) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ORF57 protein

Alternative name(s):
EB2 protein homolog

Gene names

ORF Names:

ORF57

Organism

Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus) [Reference proteome]

Taxonomic identifier

868565 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Gammaherpesvirinae › Rhadinovirus › Human herpesvirus 8

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	455 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Early protein that promotes the accumulation and nuclear export of viral intronless RNA transcripts by interacting with mRNAs and cellular export proteins. Probably acts as a viral splicing factor that regulates viral RNA splicing. Functions as a multifunctional regulator of the expression of viral lytic genes. Ref.3
Subunit structure	Homodimer. Homodimerization is required for transactivation By similarity. Interacts with host ALYREF. Associates in a complex with RNA, and host export factors NXF1/TAP and ALYREF; these interactions allow nuclear export of viral transcripts Probable. Ref.3
Subcellular location	Host cytoplasm By similarity. Host nucleus By similarity. Note: Shuttles between the nucleus and the cytoplasm.
Induction	Transactivated by ORF50 protein.
Domain	Binds viral intronless RNAs By similarity.
Miscellaneous	ORF50 and ORF57 are the earliest genes expressed in the lytic cycle.
Sequence similarities	Belongs to the HHV-1 ICP27 protein family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Host cytoplasm Host nucleus
Developmental stage	Early protein
Domain	Zinc-finger
Ligand	Metal-binding RNA-binding Zinc
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 455	455	ORF57 protein		PRO_0000387979
Regions
	Zinc finger	423 – 432	10	Potential
	Motif	101 – 107	7	Nuclear localization signal Potential
	Motif	121 – 130	10	Nuclear localization signal Potential
	Motif	143 – 152	10	Nuclear localization signal Potential
	Compositional bias	122 – 152	31	Arg-rich
Experimental info
	Sequence conflict	1 – 16	16	MVQAM…KGILE → RIVLSSSPGGPSPHPSFIAF DIT in AAB62615. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q2HR75 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: D3262C05AA472515	FASTA	455	51,012
	10 20 30 40 50 60 MVQAMIDMDI MKGILEDSVS SSEFDESRDD ETDAPTLEDE QLSEPAEPPA DERIRGTQSA 70 80 90 100 110 120 QGIPPPLGRI PKKSQGRSQL RSEIQFCSPL SRPRSPSPVN RYGKKIKFGT AGQNTRPPPE 130 140 150 160 170 180 KRPRRRPRDR LQYGRTTRGG QCRAAPKRAT RRPQVNCQRQ DDDVRQGVSD AVKKLRLPAS 190 200 210 220 230 240 MIIDGESPRF DDSIIPRHHG ACFNVFIPAP PSHVPEVFTD RDITALIRAG GKDDELINKK 250 260 270 280 290 300 ISAKKIDHLH RQMLSFVTSR HNQAYWVSCR RETAAAGGLQ TLGAFVEEQM TWAQTVVRHG 310 320 330 340 350 360 GWFDEKDIDI ILDTAIFVCN AFVTRFRLLH LSCVFDKQSE LALIKQVAYL VAMGNRLVEA 370 380 390 400 410 420 CNLLGEVKLN FRGGLLLAFV LTIPGMQSRR SISARGQELF RTLLEYYRPG DVMGLLNVIV 430 440 450 MEHHSLCRNS ECAAATRAAM GSAKFNKGLF FYPLS

References

[1]	"Kaposi's sarcoma-associated herpesvirus immune modulation: an overview." Rezaee S.A.R., Cunningham C., Davison A.J., Blackbourn D.J. J. Gen. Virol. 87:1781-1804(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome of human herpesvirus 8 cloned from Kaposi's sarcoma." Neipel F., Albrecht J.-C., Ensser A., Huang Y.-Q., Li J.J., Friedman-Kien A.E., Fleckenstein B. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The evolutionarily conserved Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with REF protein and acts as an RNA export factor." Malik P., Blackbourn D.J., Clements J.B. J. Biol. Chem. 279:33001-33011(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH HUMAN ALYREF.
[4]	"Kaposi's sarcoma-associated herpesvirus ORF57 in viral RNA processing." Majerciak V., Zheng Z.-M. Front. Biosci. 14:1516-1528(2009) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF148805 Genomic DNA. Translation: ABD28908.1. U93872 Genomic DNA. Translation: AAB62615.1.
RefSeq	YP_001129410.1. NC_009333.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-7291312.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4961525.
Phylogenomic databases
ProtClustDB	CLSP2509694.
Family and domain databases
InterPro	IPR008648. Herpes_UL69. [Graphical view]
Pfam	PF05459. Herpes_UL69. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ORF57_HHV8P

Accession

Primary (citable) accession number: Q2HR75
Secondary accession number(s): O40938

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	March 21, 2006
Last modified:	October 3, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program