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Q2HR75

- ICP27_HHV8P

UniProt

Q2HR75 - ICP27_HHV8P

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Protein

mRNA export factor ICP27 homolog

Gene

ORF57

Organism
Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Early protein that promotes the accumulation and nuclear export of viral intronless RNA transcripts by interacting with mRNAs and cellular export proteins. Probably acts as a viral splicing factor that regulates viral RNA splicing. Functions as a multifunctional regulator of the expression of viral lytic genes.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri423 – 43210Sequence Analysis

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA export factor ICP27 homolog
Alternative name(s):
EB2 protein homolog
Gene namesi
ORF Names:ORF57
OrganismiHuman herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Taxonomic identifieri868565 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000000942: Genome

Subcellular locationi

Host cytoplasm By similarity. Host nucleus By similarity
Note: Shuttles between the nucleus and the cytoplasm.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 455455mRNA export factor ICP27 homologPRO_0000387979Add
BLAST

Expressioni

Inductioni

Transactivated by ORF50 protein.

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homodimer. Homodimerization is required for transactivation (By similarity). Interacts with host ALYREF. Associates in a complex with RNA, and host export factors NXF1/TAP and ALYREF; these interactions allow nuclear export of viral transcripts (Probable). Interacts with protein K-bZIP/K8; this interaction promotes viral gene expression during lytic infection.By similarity2 PublicationsCurated

Binary interactionsi

WithEntry#Exp.IntActNotes
K8Q2HR825EBI-6884751,EBI-9006943
WIBGQ9BRP84EBI-6884751,EBI-2352802From a different organism.

Protein-protein interaction databases

BioGridi1777028. 9 interactions.
IntActiQ2HR75. 5 interactions.
MINTiMINT-7291312.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi101 – 1077Nuclear localization signalSequence Analysis
Motifi121 – 13010Nuclear localization signalSequence Analysis
Motifi143 – 15210Nuclear localization signalSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi122 – 15231Arg-richAdd
BLAST

Domaini

Binds viral intronless RNAs.By similarity

Sequence similaritiesi

Belongs to the HHV-1 ICP27 protein family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri423 – 43210Sequence Analysis

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR008648. Herpes_UL69.
[Graphical view]
PfamiPF05459. Herpes_UL69. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2HR75-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVQAMIDMDI MKGILEDSVS SSEFDESRDD ETDAPTLEDE QLSEPAEPPA
60 70 80 90 100
DERIRGTQSA QGIPPPLGRI PKKSQGRSQL RSEIQFCSPL SRPRSPSPVN
110 120 130 140 150
RYGKKIKFGT AGQNTRPPPE KRPRRRPRDR LQYGRTTRGG QCRAAPKRAT
160 170 180 190 200
RRPQVNCQRQ DDDVRQGVSD AVKKLRLPAS MIIDGESPRF DDSIIPRHHG
210 220 230 240 250
ACFNVFIPAP PSHVPEVFTD RDITALIRAG GKDDELINKK ISAKKIDHLH
260 270 280 290 300
RQMLSFVTSR HNQAYWVSCR RETAAAGGLQ TLGAFVEEQM TWAQTVVRHG
310 320 330 340 350
GWFDEKDIDI ILDTAIFVCN AFVTRFRLLH LSCVFDKQSE LALIKQVAYL
360 370 380 390 400
VAMGNRLVEA CNLLGEVKLN FRGGLLLAFV LTIPGMQSRR SISARGQELF
410 420 430 440 450
RTLLEYYRPG DVMGLLNVIV MEHHSLCRNS ECAAATRAAM GSAKFNKGLF

FYPLS
Length:455
Mass (Da):51,012
Last modified:March 21, 2006 - v1
Checksum:iD3262C05AA472515
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 1616MVQAM…KGILE → RIVLSSSPGGPSPHPSFIAF DIT in AAB62615. 1 PublicationCuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148805 Genomic DNA. Translation: ABD28908.1.
U93872 Genomic DNA. Translation: AAB62615.1.
RefSeqiYP_001129410.1. NC_009333.1.

Genome annotation databases

GeneIDi4961525.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148805 Genomic DNA. Translation: ABD28908.1 .
U93872 Genomic DNA. Translation: AAB62615.1 .
RefSeqi YP_001129410.1. NC_009333.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 1777028. 9 interactions.
IntActi Q2HR75. 5 interactions.
MINTi MINT-7291312.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4961525.

Family and domain databases

InterProi IPR008648. Herpes_UL69.
[Graphical view ]
Pfami PF05459. Herpes_UL69. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Kaposi's sarcoma-associated herpesvirus immune modulation: an overview."
    Rezaee S.A.R., Cunningham C., Davison A.J., Blackbourn D.J.
    J. Gen. Virol. 87:1781-1804(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome of human herpesvirus 8 cloned from Kaposi's sarcoma."
    Neipel F., Albrecht J.-C., Ensser A., Huang Y.-Q., Li J.J., Friedman-Kien A.E., Fleckenstein B.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The evolutionarily conserved Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with REF protein and acts as an RNA export factor."
    Malik P., Blackbourn D.J., Clements J.B.
    J. Biol. Chem. 279:33001-33011(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HUMAN ALYREF.
  4. "Kaposi's sarcoma-associated herpesvirus ORF57 in viral RNA processing."
    Majerciak V., Zheng Z.-M.
    Front. Biosci. 14:1516-1528(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  5. "Chromatin immunoprecipitation and microarray analysis suggest functional cooperation between Kaposi's Sarcoma-associated herpesvirus ORF57 and K-bZIP."
    Hunter O.V., Sei E., Richardson R.B., Conrad N.K.
    J. Virol. 87:4005-4016(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PROTEIN KBZIP/K8.

Entry informationi

Entry nameiICP27_HHV8P
AccessioniPrimary (citable) accession number: Q2HR75
Secondary accession number(s): O40938
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: March 21, 2006
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

ORF50 and ORF57 are the earliest genes expressed in the lytic cycle.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3