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Q2HR75

- ICP27_HHV8P

UniProt

Q2HR75 - ICP27_HHV8P

Protein

mRNA export factor ICP27 homolog

Gene

ORF57

Organism
Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    Early protein that promotes the accumulation and nuclear export of viral intronless RNA transcripts by interacting with mRNAs and cellular export proteins. Probably acts as a viral splicing factor that regulates viral RNA splicing. Functions as a multifunctional regulator of the expression of viral lytic genes.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri423 – 43210Sequence Analysis

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    mRNA export factor ICP27 homolog
    Alternative name(s):
    EB2 protein homolog
    Gene namesi
    ORF Names:ORF57
    OrganismiHuman herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
    Taxonomic identifieri868565 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000000942: Genome

    Subcellular locationi

    Host cytoplasm By similarity. Host nucleus By similarity
    Note: Shuttles between the nucleus and the cytoplasm.

    GO - Cellular componenti

    1. host cell cytoplasm Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cytoplasm, Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 455455mRNA export factor ICP27 homologPRO_0000387979Add
    BLAST

    Expressioni

    Inductioni

    Transactivated by ORF50 protein.

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Homodimer. Homodimerization is required for transactivation By similarity. Interacts with host ALYREF. Associates in a complex with RNA, and host export factors NXF1/TAP and ALYREF; these interactions allow nuclear export of viral transcripts Probable. Interacts with protein K-bZIP/K8; this interaction promotes viral gene expression during lytic infection.By similarity2 PublicationsCurated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    K8Q2HR825EBI-6884751,EBI-9006943
    WIBGQ9BRP84EBI-6884751,EBI-2352802From a different organism.

    Protein-protein interaction databases

    BioGridi1777028. 9 interactions.
    IntActiQ2HR75. 5 interactions.
    MINTiMINT-7291312.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi101 – 1077Nuclear localization signalSequence Analysis
    Motifi121 – 13010Nuclear localization signalSequence Analysis
    Motifi143 – 15210Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi122 – 15231Arg-richAdd
    BLAST

    Domaini

    Binds viral intronless RNAs.By similarity

    Sequence similaritiesi

    Belongs to the HHV-1 ICP27 protein family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri423 – 43210Sequence Analysis

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR008648. Herpes_UL69.
    [Graphical view]
    PfamiPF05459. Herpes_UL69. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q2HR75-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVQAMIDMDI MKGILEDSVS SSEFDESRDD ETDAPTLEDE QLSEPAEPPA    50
    DERIRGTQSA QGIPPPLGRI PKKSQGRSQL RSEIQFCSPL SRPRSPSPVN 100
    RYGKKIKFGT AGQNTRPPPE KRPRRRPRDR LQYGRTTRGG QCRAAPKRAT 150
    RRPQVNCQRQ DDDVRQGVSD AVKKLRLPAS MIIDGESPRF DDSIIPRHHG 200
    ACFNVFIPAP PSHVPEVFTD RDITALIRAG GKDDELINKK ISAKKIDHLH 250
    RQMLSFVTSR HNQAYWVSCR RETAAAGGLQ TLGAFVEEQM TWAQTVVRHG 300
    GWFDEKDIDI ILDTAIFVCN AFVTRFRLLH LSCVFDKQSE LALIKQVAYL 350
    VAMGNRLVEA CNLLGEVKLN FRGGLLLAFV LTIPGMQSRR SISARGQELF 400
    RTLLEYYRPG DVMGLLNVIV MEHHSLCRNS ECAAATRAAM GSAKFNKGLF 450
    FYPLS 455
    Length:455
    Mass (Da):51,012
    Last modified:March 21, 2006 - v1
    Checksum:iD3262C05AA472515
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 1616MVQAM…KGILE → RIVLSSSPGGPSPHPSFIAF DIT in AAB62615. 1 PublicationCuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148805 Genomic DNA. Translation: ABD28908.1.
    U93872 Genomic DNA. Translation: AAB62615.1.
    RefSeqiYP_001129410.1. NC_009333.1.

    Genome annotation databases

    GeneIDi4961525.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF148805 Genomic DNA. Translation: ABD28908.1 .
    U93872 Genomic DNA. Translation: AAB62615.1 .
    RefSeqi YP_001129410.1. NC_009333.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 1777028. 9 interactions.
    IntActi Q2HR75. 5 interactions.
    MINTi MINT-7291312.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 4961525.

    Family and domain databases

    InterProi IPR008648. Herpes_UL69.
    [Graphical view ]
    Pfami PF05459. Herpes_UL69. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Kaposi's sarcoma-associated herpesvirus immune modulation: an overview."
      Rezaee S.A.R., Cunningham C., Davison A.J., Blackbourn D.J.
      J. Gen. Virol. 87:1781-1804(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome of human herpesvirus 8 cloned from Kaposi's sarcoma."
      Neipel F., Albrecht J.-C., Ensser A., Huang Y.-Q., Li J.J., Friedman-Kien A.E., Fleckenstein B.
      Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The evolutionarily conserved Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with REF protein and acts as an RNA export factor."
      Malik P., Blackbourn D.J., Clements J.B.
      J. Biol. Chem. 279:33001-33011(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HUMAN ALYREF.
    4. "Kaposi's sarcoma-associated herpesvirus ORF57 in viral RNA processing."
      Majerciak V., Zheng Z.-M.
      Front. Biosci. 14:1516-1528(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    5. "Chromatin immunoprecipitation and microarray analysis suggest functional cooperation between Kaposi's Sarcoma-associated herpesvirus ORF57 and K-bZIP."
      Hunter O.V., Sei E., Richardson R.B., Conrad N.K.
      J. Virol. 87:4005-4016(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PROTEIN KBZIP/K8.

    Entry informationi

    Entry nameiICP27_HHV8P
    AccessioniPrimary (citable) accession number: Q2HR75
    Secondary accession number(s): O40938
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    ORF50 and ORF57 are the earliest genes expressed in the lytic cycle.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3