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Q2HR64 (DEN_HHV8P) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Alternative name(s):
Tegument protein VP1-2
Tegument protein VP1/2
Gene names
ORF Names:ORF64
OrganismHuman herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus) [Reference proteome]
Taxonomic identifier868565 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length2635 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity. Ref.2

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument. Host cytoplasm. Host nucleus By similarity. Note: Tightly associated with the capsid By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 26352635Deneddylase
PRO_0000406914

Regions

Domain9 – 223215Peptidase C76
Coiled coil1085 – 111228 Potential
Coiled coil1394 – 142734 Potential
Compositional bias300 – 32728Gly-rich
Compositional bias371 – 39323Pro-rich
Compositional bias427 – 4304Poly-Asp
Compositional bias2237 – 2536300Pro-rich

Sites

Active site291 By similarity
Active site1591 By similarity
Active site1611 By similarity
Site161Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q2HR64 [UniParc].

Last modified May 15, 2007. Version 1.
Checksum: 00070132EA8139AF

FASTA2,635289,691
        10         20         30         40         50         60 
MAAQPLYMEG MASTHQANCI FGEHAGSQCL SNCVMYLASS YYNSETPLVD RASLDDVLEQ 

        70         80         90        100        110        120 
GMRLDLLLRK SGMLGFRQYA QLHHIPGFLR TDDWATKIFQ SPEFYGLIGQ DAAIREPFIE 

       130        140        150        160        170        180 
SLRSVLSRNY AGTVQYLIII CQSKAGAIVV KDKTYYMFDP HCIPNIPNSP AHVIKTNDVG 

       190        200        210        220        230        240 
VLLPYIATHD TEYTGCFLYF IPHDYISPEH YIANHYRTIV FEELHGPRMD ISRGVESCSI 

       250        260        270        280        290        300 
TEITSPSVSP APSEAPLRRD STQSQDETRP RRPRVVIPPY DPTDRPRPPH QDRPPEQAAG 

       310        320        330        340        350        360 
YGGNKGRGGN KGRGGKTGRG GNEGRGGHQP PDEHQPPHIT AEHMDQSDGQ GADGDMDSTP 

       370        380        390        400        410        420 
ANGETSVTET PGPEPNPPAR PDREPPPTPP ATPGATALLS DLTATRGQKR KFSSLKESYP 

       430        440        450        460        470        480 
IDSPPSDDDD VSQPSQQTAP DTEDIWIDDP LTPLYPLTDT PSFDITADVT PDNTHPEKAA 

       490        500        510        520        530        540 
DGDFTNKTTS TDADRYASAS QESLGTLVSP YDFTNLDTLL AELGRLGTAQ PIPVIVDRLT 

       550        560        570        580        590        600 
SRPFREASAL QAMDRILTHV VLEYGLVSGY STAAPSKCTH VLQFFILWGE KLGIPTEDAK 

       610        620        630        640        650        660 
TLLESALEIP AMCEIVQQGR LKEPTFSRHI ISKLNPCLES LHATSRQDFK SLIQAFNAEG 

       670        680        690        700        710        720 
IRIASRERET SMAELIETIT ARLKPNFNIV CARQDAQTIQ DGVGLLRAEV NKRNAQIAQE 

       730        740        750        760        770        780 
AAYFENIITA LSTFQPPPQS QQTFEVLPDL KLRTLVEHLT LVEAQVTTQT VESLQAYLQS 

       790        800        810        820        830        840 
AATAEHHLTN VPNVHSILSN ISNTLKVIDY VIPKFIINTD TLAPYKQQFS YLGGELASMF 

       850        860        870        880        890        900 
SLDWPHAPAE AVEPLPVLTS LRGKIAEALT RQENKNAVDQ ILTDAEGLLK NITDPNGAHF 

       910        920        930        940        950        960 
HAQAVSIPVL ENYVHNAGVL LKGEKSERFS RLKTAIQNLV SSESFITVTL HSTNLGNLVT 

       970        980        990       1000       1010       1020 
NVPKLGEAFT GGPHLLTSPS VRQSLSTLCT TLLRDALDAL EKKDPALLGE GTTLALETLL 

      1030       1040       1050       1060       1070       1080 
GYGSVQDYKE TVQIISSLVG IQKLVRDQGA DKWATAVTRL TDLKSTLATT AIETATKRKL 

      1090       1100       1110       1120       1130       1140 
YRLIQRDLKE AQKHETNRAM EEWKQKVLAL DNASPERVAT LLQQAPTAKA REFAEKHFKI 

      1150       1160       1170       1180       1190       1200 
LLPVPADAPV QASPTPMEYS ASPLPDPKDI DRATSIHGEQ AWKKIQQAFK DFNFAVLRPA 

      1210       1220       1230       1240       1250       1260 
DWDALAAEYQ RRGSPLPAAV GPALSGFLET ILGTLNDIYM DKLRSFLPDA QPFQAPPFDW 

      1270       1280       1290       1300       1310       1320 
LTPYQDQVSF FLRTIGLPLV RALADKISVQ ALRLSHALQS GDLQQATVGT PLELPATEYA 

      1330       1340       1350       1360       1370       1380 
RIASNMKSVF NDHGLQVRSE VADYVEAQRA DAHTPHVPRP KIQAPKTLIP HPDAIVADGL 

      1390       1400       1410       1420       1430       1440 
PAFLKTSLLQ QEAKLLALQR ADFESLESDM RAAEAQRKAS REETQRKMAH AITQLLQQAP 

      1450       1460       1470       1480       1490       1500 
SAISGRPLSL QDPVGFLEGI IYDKVLERES YETGLEGLSW LEQTIKSITV YAPVEEKQRM 

      1510       1520       1530       1540       1550       1560 
HVLLDEVKKQ RANTETALEL EAAATHGDDA RLLQRAVDEL SPLRVKGGKA AVESWRQKIQ 

      1570       1580       1590       1600       1610       1620 
TLKSLVQEAE QAGLLLATID TVAGQAQETI SPSTLQGLYQ QGQEAMAAIK RFRDSPQLAG 

      1630       1640       1650       1660       1670       1680 
LQEKLAELQQ YVKYKKQYLE HFEATQSVVF TAFPLTQEVT IPALHYAGPF DNLERLSRYL 

      1690       1700       1710       1720       1730       1740 
HIGQTQPAPG QWLLTLPTFD PTRPACVPAG GHEPPLHRQV VFSSFLEAQI RLALSVAGPV 

      1750       1760       1770       1780       1790       1800 
PGRGLPGTPQ IRRGVEAAAC FLHQWDEISR LLPEVLDTFF HNAPLPAESS SNAFLAMCVL 

      1810       1820       1830       1840       1850       1860 
THLVYLAGRA VLGPREPEHA APDAYPREVA LAPRDLTYLL LAMWPSWISA ILKQPSHAEA 

      1870       1880       1890       1900       1910       1920 
AHACLVTLPT MLKAVPYLTL EASAGPLPAD MRHFATPEAR LFFPARWHHV NVQEKLWLRN 

      1930       1940       1950       1960       1970       1980 
DFMSLCHRSP GRARIAVLVW AVTCLDPEVI RQLWSTLRPL TADESDTASG LLRVLVEMEF 

      1990       2000       2010       2020       2030       2040 
GPPPKTPRRE AVAPGATLPP YPYGLATGER LVGQAQERSG GAGKMPVSGF EIVLGALLFR 

      2050       2060       2070       2080       2090       2100 
APLRIFSTAS THRISDFEGG FQILTPLLDC CPDREPFASL AAAPRRTVPL GDPCANIHTP 

      2110       2120       2130       2140       2150       2160 
EEIQIFARQA AWLQYTFANY QIPSTDNPIP IVVLNANNNL ENSYIPRDRK ADPLRPFYVV 

      2170       2180       2190       2200       2210       2220 
PLKPQGRWPE IMTTATTPCR LPTSPEEAGS QFARLLQSQV SATWSDIFSR VPERLAPNAP 

      2230       2240       2250       2260       2270       2280 
QKSSQTMSEI HEVAATPPLT ITPNKPTGTP HVSPEADPIT ERKRGQQPKI VADNMPSRIL 

      2290       2300       2310       2320       2330       2340 
PSLPTPKPRE PRITLPHALP VISPPAHRPS PIPHLPAPQV TEPKGVLQSK RGTLVLRPAA 

      2350       2360       2370       2380       2390       2400 
VIDPRKPVSA PITRYERTAL QPPRTEGEGR RPPDTQPVTL TFRLPPTAPT PATAALETKT 

      2410       2420       2430       2440       2450       2460 
TPPSTPPHAI DISPPQTPPM STSPHARDTS PPAEKRAAPV IRVMAPTQPS GEARVKRVEI 

      2470       2480       2490       2500       2510       2520 
EQGLSTRNEA PPLERSNHAV PAVTPRRTVA REIRIPPEIK AGWDTAPDIP LPHSSPESSP 

      2530       2540       2550       2560       2570       2580 
PTSPQPIRVD DKSPLPNLVE RYARGFLDTP SVEVMSLENQ DIAVDPGLLT RRIPSVVPMP 

      2590       2600       2610       2620       2630 
HPIMWSPIVP ISLQNTDIDT AKITLISFIR RIKQKVAALS ASLAETVDRI KKWYL 

« Hide

References

« Hide 'large scale' references
[1]"Kaposi's sarcoma-associated herpesvirus immune modulation: an overview."
Rezaee S.A.R., Cunningham C., Davison A.J., Blackbourn D.J.
J. Gen. Virol. 87:1781-1804(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]"Kaposi's sarcoma-associated herpesvirus encodes a viral deubiquitinase."
Gonzalez C.M., Wang L., Damania B.
J. Virol. 83:10224-10233(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF148805 Genomic DNA. Translation: ABD28919.1.
RefSeqYP_001129421.1. NC_009333.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ2HR64. 3 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4961441.

Phylogenomic databases

ProtClustDBCLSP2509700.

Family and domain databases

InterProIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_HHV8P
AccessionPrimary (citable) accession number: Q2HR64
Entry history
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: May 15, 2007
Last modified: October 16, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries