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Q2HR64

- DEN_HHV8P

UniProt

Q2HR64 - DEN_HHV8P

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Protein

Deneddylase

Gene

ORF64

Organism
Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei16 – 161Important for catalytic activityPROSITE-ProRule annotation
Active sitei29 – 291PROSITE-ProRule annotation
Active sitei159 – 1591PROSITE-ProRule annotation
Active sitei161 – 1611PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Alternative name(s):
Tegument protein VP1-2
Tegument protein VP1/2
Gene namesi
ORF Names:ORF64
OrganismiHuman herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi's sarcoma-associated herpesvirus)
Taxonomic identifieri868565 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000000942: Genome

Subcellular locationi

Virion tegument. Host cytoplasm. Host nucleus By similarity
Note: Tightly associated with the capsid.By similarity

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 26352635DeneddylasePRO_0000406914Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

Protein-protein interaction databases

IntActiQ2HR64. 3 interactions.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 223215Peptidase C76PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1085 – 111228Sequence AnalysisAdd
BLAST
Coiled coili1394 – 142734Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi300 – 32728Gly-richAdd
BLAST
Compositional biasi371 – 39323Pro-richAdd
BLAST
Compositional biasi427 – 4304Poly-Asp
Compositional biasi2237 – 2536300Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q2HR64-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAQPLYMEG MASTHQANCI FGEHAGSQCL SNCVMYLASS YYNSETPLVD
60 70 80 90 100
RASLDDVLEQ GMRLDLLLRK SGMLGFRQYA QLHHIPGFLR TDDWATKIFQ
110 120 130 140 150
SPEFYGLIGQ DAAIREPFIE SLRSVLSRNY AGTVQYLIII CQSKAGAIVV
160 170 180 190 200
KDKTYYMFDP HCIPNIPNSP AHVIKTNDVG VLLPYIATHD TEYTGCFLYF
210 220 230 240 250
IPHDYISPEH YIANHYRTIV FEELHGPRMD ISRGVESCSI TEITSPSVSP
260 270 280 290 300
APSEAPLRRD STQSQDETRP RRPRVVIPPY DPTDRPRPPH QDRPPEQAAG
310 320 330 340 350
YGGNKGRGGN KGRGGKTGRG GNEGRGGHQP PDEHQPPHIT AEHMDQSDGQ
360 370 380 390 400
GADGDMDSTP ANGETSVTET PGPEPNPPAR PDREPPPTPP ATPGATALLS
410 420 430 440 450
DLTATRGQKR KFSSLKESYP IDSPPSDDDD VSQPSQQTAP DTEDIWIDDP
460 470 480 490 500
LTPLYPLTDT PSFDITADVT PDNTHPEKAA DGDFTNKTTS TDADRYASAS
510 520 530 540 550
QESLGTLVSP YDFTNLDTLL AELGRLGTAQ PIPVIVDRLT SRPFREASAL
560 570 580 590 600
QAMDRILTHV VLEYGLVSGY STAAPSKCTH VLQFFILWGE KLGIPTEDAK
610 620 630 640 650
TLLESALEIP AMCEIVQQGR LKEPTFSRHI ISKLNPCLES LHATSRQDFK
660 670 680 690 700
SLIQAFNAEG IRIASRERET SMAELIETIT ARLKPNFNIV CARQDAQTIQ
710 720 730 740 750
DGVGLLRAEV NKRNAQIAQE AAYFENIITA LSTFQPPPQS QQTFEVLPDL
760 770 780 790 800
KLRTLVEHLT LVEAQVTTQT VESLQAYLQS AATAEHHLTN VPNVHSILSN
810 820 830 840 850
ISNTLKVIDY VIPKFIINTD TLAPYKQQFS YLGGELASMF SLDWPHAPAE
860 870 880 890 900
AVEPLPVLTS LRGKIAEALT RQENKNAVDQ ILTDAEGLLK NITDPNGAHF
910 920 930 940 950
HAQAVSIPVL ENYVHNAGVL LKGEKSERFS RLKTAIQNLV SSESFITVTL
960 970 980 990 1000
HSTNLGNLVT NVPKLGEAFT GGPHLLTSPS VRQSLSTLCT TLLRDALDAL
1010 1020 1030 1040 1050
EKKDPALLGE GTTLALETLL GYGSVQDYKE TVQIISSLVG IQKLVRDQGA
1060 1070 1080 1090 1100
DKWATAVTRL TDLKSTLATT AIETATKRKL YRLIQRDLKE AQKHETNRAM
1110 1120 1130 1140 1150
EEWKQKVLAL DNASPERVAT LLQQAPTAKA REFAEKHFKI LLPVPADAPV
1160 1170 1180 1190 1200
QASPTPMEYS ASPLPDPKDI DRATSIHGEQ AWKKIQQAFK DFNFAVLRPA
1210 1220 1230 1240 1250
DWDALAAEYQ RRGSPLPAAV GPALSGFLET ILGTLNDIYM DKLRSFLPDA
1260 1270 1280 1290 1300
QPFQAPPFDW LTPYQDQVSF FLRTIGLPLV RALADKISVQ ALRLSHALQS
1310 1320 1330 1340 1350
GDLQQATVGT PLELPATEYA RIASNMKSVF NDHGLQVRSE VADYVEAQRA
1360 1370 1380 1390 1400
DAHTPHVPRP KIQAPKTLIP HPDAIVADGL PAFLKTSLLQ QEAKLLALQR
1410 1420 1430 1440 1450
ADFESLESDM RAAEAQRKAS REETQRKMAH AITQLLQQAP SAISGRPLSL
1460 1470 1480 1490 1500
QDPVGFLEGI IYDKVLERES YETGLEGLSW LEQTIKSITV YAPVEEKQRM
1510 1520 1530 1540 1550
HVLLDEVKKQ RANTETALEL EAAATHGDDA RLLQRAVDEL SPLRVKGGKA
1560 1570 1580 1590 1600
AVESWRQKIQ TLKSLVQEAE QAGLLLATID TVAGQAQETI SPSTLQGLYQ
1610 1620 1630 1640 1650
QGQEAMAAIK RFRDSPQLAG LQEKLAELQQ YVKYKKQYLE HFEATQSVVF
1660 1670 1680 1690 1700
TAFPLTQEVT IPALHYAGPF DNLERLSRYL HIGQTQPAPG QWLLTLPTFD
1710 1720 1730 1740 1750
PTRPACVPAG GHEPPLHRQV VFSSFLEAQI RLALSVAGPV PGRGLPGTPQ
1760 1770 1780 1790 1800
IRRGVEAAAC FLHQWDEISR LLPEVLDTFF HNAPLPAESS SNAFLAMCVL
1810 1820 1830 1840 1850
THLVYLAGRA VLGPREPEHA APDAYPREVA LAPRDLTYLL LAMWPSWISA
1860 1870 1880 1890 1900
ILKQPSHAEA AHACLVTLPT MLKAVPYLTL EASAGPLPAD MRHFATPEAR
1910 1920 1930 1940 1950
LFFPARWHHV NVQEKLWLRN DFMSLCHRSP GRARIAVLVW AVTCLDPEVI
1960 1970 1980 1990 2000
RQLWSTLRPL TADESDTASG LLRVLVEMEF GPPPKTPRRE AVAPGATLPP
2010 2020 2030 2040 2050
YPYGLATGER LVGQAQERSG GAGKMPVSGF EIVLGALLFR APLRIFSTAS
2060 2070 2080 2090 2100
THRISDFEGG FQILTPLLDC CPDREPFASL AAAPRRTVPL GDPCANIHTP
2110 2120 2130 2140 2150
EEIQIFARQA AWLQYTFANY QIPSTDNPIP IVVLNANNNL ENSYIPRDRK
2160 2170 2180 2190 2200
ADPLRPFYVV PLKPQGRWPE IMTTATTPCR LPTSPEEAGS QFARLLQSQV
2210 2220 2230 2240 2250
SATWSDIFSR VPERLAPNAP QKSSQTMSEI HEVAATPPLT ITPNKPTGTP
2260 2270 2280 2290 2300
HVSPEADPIT ERKRGQQPKI VADNMPSRIL PSLPTPKPRE PRITLPHALP
2310 2320 2330 2340 2350
VISPPAHRPS PIPHLPAPQV TEPKGVLQSK RGTLVLRPAA VIDPRKPVSA
2360 2370 2380 2390 2400
PITRYERTAL QPPRTEGEGR RPPDTQPVTL TFRLPPTAPT PATAALETKT
2410 2420 2430 2440 2450
TPPSTPPHAI DISPPQTPPM STSPHARDTS PPAEKRAAPV IRVMAPTQPS
2460 2470 2480 2490 2500
GEARVKRVEI EQGLSTRNEA PPLERSNHAV PAVTPRRTVA REIRIPPEIK
2510 2520 2530 2540 2550
AGWDTAPDIP LPHSSPESSP PTSPQPIRVD DKSPLPNLVE RYARGFLDTP
2560 2570 2580 2590 2600
SVEVMSLENQ DIAVDPGLLT RRIPSVVPMP HPIMWSPIVP ISLQNTDIDT
2610 2620 2630
AKITLISFIR RIKQKVAALS ASLAETVDRI KKWYL
Length:2,635
Mass (Da):289,691
Last modified:May 15, 2007 - v1
Checksum:i00070132EA8139AF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148805 Genomic DNA. Translation: ABD28919.1.
RefSeqiYP_001129421.1. NC_009333.1.

Genome annotation databases

GeneIDi4961441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148805 Genomic DNA. Translation: ABD28919.1 .
RefSeqi YP_001129421.1. NC_009333.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q2HR64. 3 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4961441.

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Kaposi's sarcoma-associated herpesvirus immune modulation: an overview."
    Rezaee S.A.R., Cunningham C., Davison A.J., Blackbourn D.J.
    J. Gen. Virol. 87:1781-1804(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Kaposi's sarcoma-associated herpesvirus encodes a viral deubiquitinase."
    Gonzalez C.M., Wang L., Damania B.
    J. Virol. 83:10224-10233(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDEN_HHV8P
AccessioniPrimary (citable) accession number: Q2HR64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 5, 2011
Last sequence update: May 15, 2007
Last modified: October 29, 2014
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3