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Protein

Diphthine--ammonia ligase

Gene

DPH6

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor eEF-2 (EEF2) to diphthamide (By similarity).By similarity

Catalytic activityi

ATP + diphthine-[translation elongation factor 2] + NH3 = AMP + diphosphate + diphthamide-[translation elongation factor 2].

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-5358493. Synthesis of diphthamide-EEF2.
UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthine--ammonia ligase (EC:6.3.1.14)
Alternative name(s):
ATP-binding domain-containing protein 4
Diphthamide synthase
Diphthamide synthetase
Protein DPH6 homolog
Gene namesi
Name:DPH6
Synonyms:ATPBD4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Diphthine--ammonia ligasePRO_0000282396Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei97 – 971PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2HJF5.
PRIDEiQ2HJF5.

Expressioni

Gene expression databases

BgeeiENSBTAG00000001364.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000051676.

Structurei

3D structure databases

ProteinModelPortaliQ2HJF5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Diphthine--ammonia ligase family.Curated

Phylogenomic databases

eggNOGiKOG2316. Eukaryota.
COG2102. LUCA.
GeneTreeiENSGT00420000029820.
HOVERGENiHBG100452.
InParanoidiQ2HJF5.
KOiK06927.
OMAiYGLGKEW.
OrthoDBiEOG091G02O5.
TreeFamiTF313566.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR002761. Diphthami_syn_dom.
IPR030662. DPH6/MJ0570.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR12196. PTHR12196. 1 hit.
PfamiPF01902. Diphthami_syn_2. 1 hit.
[Graphical view]
PIRSFiPIRSF039123. Diphthamide_synthase. 1 hit.
TIGRFAMsiTIGR00290. MJ0570_dom. 1 hit.

Sequencei

Sequence statusi: Complete.

Q2HJF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVAALISGG KDSCYNMMQC VAAGHQIVAL ANLRPAENQV GSDELDSYMY
60 70 80 90 100
QTVGHHAIDL YAEAMALPLY RRTIRGKSVD TGPVYTKCEG DEVEDLYELL
110 120 130 140 150
KLVKEKEEVE GISVGAILSD YQRVRVENVC KRLNLQPLAY LWQRNQEDLL
160 170 180 190 200
QEMISSNIQA IIIKVAALGL DPDKHLGKPL DQMEPYLLEL SKKYGVHVCG
210 220 230 240 250
EGGEYETFTL DCPLFKKKII VDSSEVVTHS ADAFAPVAYL RFLELHLEDK
260
VSPVPDNCRT SNDIHNS
Length:267
Mass (Da):29,860
Last modified:March 21, 2006 - v1
Checksum:iAA38E463F4A819E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105462 mRNA. Translation: AAI05463.1.
RefSeqiNP_001070484.1. NM_001077016.1.
UniGeneiBt.42412.

Genome annotation databases

EnsembliENSBTAT00000055382; ENSBTAP00000051676; ENSBTAG00000001364.
GeneIDi767944.
KEGGibta:767944.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105462 mRNA. Translation: AAI05463.1.
RefSeqiNP_001070484.1. NM_001077016.1.
UniGeneiBt.42412.

3D structure databases

ProteinModelPortaliQ2HJF5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000051676.

Proteomic databases

PaxDbiQ2HJF5.
PRIDEiQ2HJF5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000055382; ENSBTAP00000051676; ENSBTAG00000001364.
GeneIDi767944.
KEGGibta:767944.

Organism-specific databases

CTDi89978.

Phylogenomic databases

eggNOGiKOG2316. Eukaryota.
COG2102. LUCA.
GeneTreeiENSGT00420000029820.
HOVERGENiHBG100452.
InParanoidiQ2HJF5.
KOiK06927.
OMAiYGLGKEW.
OrthoDBiEOG091G02O5.
TreeFamiTF313566.

Enzyme and pathway databases

UniPathwayiUPA00559.
ReactomeiR-BTA-5358493. Synthesis of diphthamide-EEF2.

Gene expression databases

BgeeiENSBTAG00000001364.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
InterProiIPR002761. Diphthami_syn_dom.
IPR030662. DPH6/MJ0570.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR12196. PTHR12196. 1 hit.
PfamiPF01902. Diphthami_syn_2. 1 hit.
[Graphical view]
PIRSFiPIRSF039123. Diphthamide_synthase. 1 hit.
TIGRFAMsiTIGR00290. MJ0570_dom. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPH6_BOVIN
AccessioniPrimary (citable) accession number: Q2HJF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 21, 2006
Last modified: September 7, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.