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Protein

Diphthine--ammonia ligase

Gene

DPH6

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor eEF-2 (EEF2) to diphthamide (By similarity).By similarity

Catalytic activityi

ATP + diphthine-[translation elongation factor 2] + NH3 = AMP + diphosphate + diphthamide-[translation elongation factor 2].

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-5358493 Synthesis of diphthamide-EEF2
UniPathwayiUPA00559

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthine--ammonia ligase (EC:6.3.1.14)
Alternative name(s):
ATP-binding domain-containing protein 4
Diphthamide synthase
Diphthamide synthetase
Protein DPH6 homolog
Gene namesi
Name:DPH6
Synonyms:ATPBD4
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 10

Organism-specific databases

VGNCiVGNC:28181 DPH6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002823961 – 267Diphthine--ammonia ligaseAdd BLAST267

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97PhosphotyrosineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ2HJF5
PRIDEiQ2HJF5

Expressioni

Gene expression databases

BgeeiENSBTAG00000001364

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000051676

Structurei

3D structure databases

ProteinModelPortaliQ2HJF5
SMRiQ2HJF5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Diphthine--ammonia ligase family.Curated

Phylogenomic databases

eggNOGiKOG2316 Eukaryota
COG2102 LUCA
GeneTreeiENSGT00420000029820
HOVERGENiHBG100452
InParanoidiQ2HJF5
KOiK06927
OMAiLDESWLG
OrthoDBiEOG091G02O5
TreeFamiTF313566

Family and domain databases

CDDicd01994 Alpha_ANH_like_IV, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR002761 Diphthami_syn_dom
IPR030662 DPH6/MJ0570
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR12196 PTHR12196, 1 hit
PfamiView protein in Pfam
PF01902 Diphthami_syn_2, 1 hit
PIRSFiPIRSF039123 Diphthamide_synthase, 1 hit
TIGRFAMsiTIGR00290 MJ0570_dom, 1 hit

Sequencei

Sequence statusi: Complete.

Q2HJF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVAALISGG KDSCYNMMQC VAAGHQIVAL ANLRPAENQV GSDELDSYMY
60 70 80 90 100
QTVGHHAIDL YAEAMALPLY RRTIRGKSVD TGPVYTKCEG DEVEDLYELL
110 120 130 140 150
KLVKEKEEVE GISVGAILSD YQRVRVENVC KRLNLQPLAY LWQRNQEDLL
160 170 180 190 200
QEMISSNIQA IIIKVAALGL DPDKHLGKPL DQMEPYLLEL SKKYGVHVCG
210 220 230 240 250
EGGEYETFTL DCPLFKKKII VDSSEVVTHS ADAFAPVAYL RFLELHLEDK
260
VSPVPDNCRT SNDIHNS
Length:267
Mass (Da):29,860
Last modified:March 21, 2006 - v1
Checksum:iAA38E463F4A819E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC105462 mRNA Translation: AAI05463.1
RefSeqiNP_001070484.1, NM_001077016.1
UniGeneiBt.42412

Genome annotation databases

EnsembliENSBTAT00000055382; ENSBTAP00000051676; ENSBTAG00000001364
GeneIDi767944
KEGGibta:767944

Similar proteinsi

Entry informationi

Entry nameiDPH6_BOVIN
AccessioniPrimary (citable) accession number: Q2HJF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: March 21, 2006
Last modified: May 23, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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