Reviewed, UniProtKB/Swiss-Prot Q2HJD7 (3HIDH_BOVIN)

Names and origin

Protein names

Recommended name:
    3-hydroxyisobutyrate dehydrogenase, mitochondrial
      Short name=HIBADH
    EC=1.1.1.31

Gene names

Name:

HIBADH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the 3-hydroxyisobutyrate dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Mitochondrion By similarity
	Chain	37 – 336	300	3-hydroxyisobutyrate dehydrogenase, mitochondrial		PRO_0000290342
Regions
	Nucleotide binding	40 – 69	30	NAD By similarity
	Nucleotide binding	103 – 104	2	NAD By similarity
Sites
	Active site	209	1	By similarity
	Binding site	108	1	NAD By similarity
	Binding site	134	1	NAD; via amide nitrogen By similarity
	Binding site	284	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q2HJD7-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: 11AB54F01BA8D5B8	FASTA	336	35,410
	10 20 30 40 50 60 MAASLRLRGA ASGLRYWSRR QPPAVASLAA VCSRSMASKT PVGFIGVGNM GNPMAKNLMK 70 80 90 100 110 120 HGYPLIIYDV FPDACKEFLD AGEQVVSSPA DVAEKADRII TMLPTSINAI EAYSGANGIL 130 140 150 160 170 180 KKVKKGSLLI DSSTIDPMVS KELAKEVEKM GAVFMDAPVS GGVGAARSGN LTFMVGGVEE 190 200 210 220 230 240 EFAAAQELLG CMGSNVVYCG AVGTGQAAKI CNNLLLAISM IGTAEAMNLG IRLGLDPKLL 250 260 270 280 290 300 AKILNMSSGR CWSSDTYNPV PGVMDGVPSA NNYQGGFGTT LMAKDLGLAQ DSATSTKSPI 310 320 330 LLGSQAHQIY RMMCAKGYSK KDFSSVFQFL REEETF

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Ascending colon.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC105543 mRNA. Translation: AAI05544.1.
IPI	IPI00712360.
RefSeq	NP_001039571.1.
UniGene	Bt.23720
3D structure databases
SMR	Q2HJD7. Positions 41-335.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000001036. Bos taurus. [Contig view]
GeneID	512002.
KEGG	bta:512002.
Phylogenomic databases
HOVERGEN	Q2HJD7.
OMA	Q2HJD7. PMARNLI.
Enzyme and pathway databases
BRENDA	1.1.1.31. 251.
Family and domain databases
InterPro	IPR002204. 3-OH-isobutyrate_DH-rel_CS. IPR015815. 3hydroxyacid_DH/Rdtase. IPR006183. 6-phosphogluconate_DH. IPR006115. 6PGDH_NAD-bd. IPR013328. DH_multihelical. IPR011548. IsoBut3OH_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR22981. 3hydroxy_acid_DH. 1 hit.
Pfam	PF03446. NAD_binding_2. 1 hit. [Graphical view]
PRINTS	PR00076. 6PGDHDRGNASE.
TIGRFAMs	TIGR01692. HIBADH. 1 hit.
PROSITE	PS00895. 3_HYDROXYISOBUT_DH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

3HIDH_BOVIN

Accession

Primary (citable) accession number: Q2HJD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	March 21, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)