Reviewed, UniProtKB/Swiss-Prot Q2HJ89 (FKB10_BOVIN)

Names and origin

Protein names

Recommended name:
    FK506-binding protein 10
    EC=5.2.1.8
Alternative name(s):
    Peptidyl-prolyl cis-trans isomerase
      Short name=PPIase
      Short name=Rotamase

Gene names

Name:

FKBP10

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	PPIases accelerate the folding of proteins during protein synthesis.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Enzyme regulation	Inhibited by both FK506 and rapamycin, but not by cyclosporin A By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Post-translational modification	Glycosylated and phosphorylated By similarity.
Sequence similarities	Contains 2 EF-hand domains. Contains 4 PPIase FKBP-type domains.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium
Molecular function	Isomerase Rotamase
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-prolyl cis-trans isomerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 583	556	FK506-binding protein 10		PRO_0000285594
Regions
	Domain	63 – 151	89	PPIase FKBP-type 1
	Domain	175 – 263	89	PPIase FKBP-type 2
	Domain	287 – 375	89	PPIase FKBP-type 3
	Domain	400 – 487	88	PPIase FKBP-type 4
	Domain	498 – 533	36	EF-hand 1
	Domain	543 – 578	36	EF-hand 2
	Calcium binding	511 – 522	12	1 Potential
	Calcium binding	556 – 567	12	2 Potential
	Motif	580 – 583	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Glycosylation	183	1	N-linked (GlcNAc...) Potential
	Glycosylation	295	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q2HJ89-1 [UniParc]. Last modified March 21, 2006. Version 1. Checksum: E651201D6D74D5F6	FASTA	583	64,484
	10 20 30 40 50 60 MLRAGPPSHT LLRLPLLQLL LLLLVQAVGR GLGRASPAGG PLEDVVIERY HIPRVCPREV 70 80 90 100 110 120 QMGDFVRYHY NGTFEDGKKF DSSYDRHTLV AIVVGVGRLI TGMDRGLMGM CVNERRRLIV 130 140 150 160 170 180 PPHLGYGSIG VAGLIPPDAT LYFDVVLLDV WNKEDTVQVS TLLRPPHCPR MVQDSDFVRY 190 200 210 220 230 240 HYNGTLLDGT AFDTSYSKGG TYDTYVGSGW LIKGMDQGLL GMCPGERRKI VIPPFLAYGE 250 260 270 280 290 300 KGYGTVIPSQ ASLVFHVLLI DVHNPKDTVQ LETLELPPGC VRRAVAGDFM RYHYNGSLMD 310 320 330 340 350 360 GTLFDSSYSR NHTYNTYVGQ GYIIPGMDQG LQGSCMGERR RITIPPHLAY GENGTGDKIP 370 380 390 400 410 420 GSAVLIFDVH VIDFHNPADP VEIKTLSRPL ETCNETAKLG DFVHYHYNCS LLDGTRLFSS 430 440 450 460 470 480 HDYGAPQEAT LGAHKVIEGL DTGLQGMCVG ERRQLVVPPH LAHGESGARG VPGSAVLLFE 490 500 510 520 530 540 VELVSREDGL PTGYLFVWHE DPPAHLFEHM DLNKDGEVPV EEFSTFIKAQ VSEGKGRLLP 550 560 570 580 GQDPEKTIGD MFQNQDRNQD GKITAEELKL KSDEDQDRVH EEL

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC113250 mRNA. Translation: AAI13251.1.
IPI	IPI00697856.
RefSeq	NP_001039868.1.
UniGene	Bt.2698
3D structure databases
HSSP	HSSP built from PDB template 2DG4 based on UniProtKB P62942.
SMR	Q2HJ89. Positions 61-276, 173-427, 398-485.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000015220; ENSBTAP00000015220; ENSBTAG00000011454; Bos taurus. [Genome view]
GeneID	535310.
KEGG	bta:535310.
Organism-specific databases
CTD	535310.
Phylogenomic databases
eggNOG	maNOG05965.
HOVERGEN	Q2HJ89.
InParanoid	Q2HJ89.
OMA	VQDSDFV.
OrthoDB	EOG9MSGHW.
PhylomeDB	Q2HJ89.
Enzyme and pathway databases
BRENDA	5.2.1.8. 251.
Family and domain databases
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR001179. PPIase_FKBP. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
PANTHER	PTHR10516. PPIase_FKBP. 1 hit.
Pfam	PF00254. FKBP_C. 4 hits. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. PS00014. ER_TARGET. 1 hit. PS50059. FKBP_PPIASE. 4 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FKB10_BOVIN

Accession

Primary (citable) accession number: Q2HJ89

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	March 21, 2006
Last modified:	January 19, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)