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Protein

Glycine amidinotransferase, mitochondrial

Gene

GATM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the biosynthesis of guanidinoacetate, the immediate precursor of creatine. Creatine plays a vital role in energy metabolism in muscle tissues. May play a role in embryonic and central nervous system development (By similarity).By similarity

Catalytic activityi

L-arginine + glycine = L-ornithine + guanidinoacetate.

Pathwayi: creatine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes creatine from L-arginine and glycine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glycine amidinotransferase, mitochondrial (GATM)
  2. Guanidinoacetate N-methyltransferase (GAMT)
This subpathway is part of the pathway creatine biosynthesis, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes creatine from L-arginine and glycine, the pathway creatine biosynthesis and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei254 – 2541By similarity
Active sitei303 – 3031By similarity
Active sitei407 – 4071Amidino-cysteine intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

ReactomeiR-BTA-71288. Creatine metabolism.
UniPathwayiUPA00104; UER00579.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine amidinotransferase, mitochondrial (EC:2.1.4.1)
Alternative name(s):
L-arginine:glycine amidinotransferase
Transamidinase
Gene namesi
Name:GATM
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3737MitochondrionBy similarityAdd
BLAST
Chaini38 – 423386Glycine amidinotransferase, mitochondrialPRO_0000286940Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei46 – 461PhosphoserineBy similarity
Modified residuei49 – 491PhosphoserineBy similarity
Modified residuei385 – 3851N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ2HJ74.
PeptideAtlasiQ2HJ74.
PRIDEiQ2HJ74.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007338.

Structurei

3D structure databases

ProteinModelPortaliQ2HJ74.
SMRiQ2HJ74. Positions 64-423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidinotransferase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IFBR. Eukaryota.
ENOG410Y45M. LUCA.
GeneTreeiENSGT00390000011613.
HOGENOMiHOG000231593.
HOVERGENiHBG002492.
InParanoidiQ2HJ74.
KOiK00613.
OMAiRPCHQID.
OrthoDBiEOG712TW4.
TreeFamiTF300256.

Family and domain databases

InterProiIPR033195. AmidinoTrfase.
[Graphical view]
PANTHERiPTHR10488. PTHR10488. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q2HJ74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRVRCLRGG SRGAEALHYI GSRLGRTVTG WVQRTFQSTQ AATASSRNSC
60 70 80 90 100
AADDKATDPL PKDCPVSSFN EWDPLEEVIV GRAENACVPP FTVEVKANTY
110 120 130 140 150
DKHWPFYQKY GGSYFPKDHL QKAVAEIEEM CNILKMEGVT VRRPDPIDWS
160 170 180 190 200
LKYKTPDFES TGLYGAMPRD ILIVVGNEII EAPMAWRARF FEYRAYRTII
210 220 230 240 250
KDYFRRGAKW TTAPKPTMAD ELYDQDYPIH SVEDRHKLAA QGKFVTTEFE
260 270 280 290 300
PCFDAADFIR AGRDIFVQRS QVTNYMGIEW MRKHLAPDYR VHIVSFKDPN
310 320 330 340 350
PMHIDATFNI IGPGLVLSNP DRPCHQIDLF KKAGWTIVTP PTPIIPDDHP
360 370 380 390 400
LWMSSKWLSM NVLMLDEKRV MVDANEVPIQ KMFEKLGIST IKVSIRNANS
410 420
LGGGFHCWTC DVRRRGTLQS YFD
Length:423
Mass (Da):48,357
Last modified:March 21, 2006 - v1
Checksum:iA330454DB84EE5BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC113272 mRNA. Translation: AAI13273.1.
RefSeqiNP_001039343.1. NM_001045878.1.
UniGeneiBt.30785.

Genome annotation databases

EnsembliENSBTAT00000007338; ENSBTAP00000007338; ENSBTAG00000005586.
GeneIDi414732.
KEGGibta:414732.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC113272 mRNA. Translation: AAI13273.1.
RefSeqiNP_001039343.1. NM_001045878.1.
UniGeneiBt.30785.

3D structure databases

ProteinModelPortaliQ2HJ74.
SMRiQ2HJ74. Positions 64-423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007338.

Proteomic databases

PaxDbiQ2HJ74.
PeptideAtlasiQ2HJ74.
PRIDEiQ2HJ74.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007338; ENSBTAP00000007338; ENSBTAG00000005586.
GeneIDi414732.
KEGGibta:414732.

Organism-specific databases

CTDi2628.

Phylogenomic databases

eggNOGiENOG410IFBR. Eukaryota.
ENOG410Y45M. LUCA.
GeneTreeiENSGT00390000011613.
HOGENOMiHOG000231593.
HOVERGENiHBG002492.
InParanoidiQ2HJ74.
KOiK00613.
OMAiRPCHQID.
OrthoDBiEOG712TW4.
TreeFamiTF300256.

Enzyme and pathway databases

UniPathwayiUPA00104; UER00579.
ReactomeiR-BTA-71288. Creatine metabolism.

Family and domain databases

InterProiIPR033195. AmidinoTrfase.
[Graphical view]
PANTHERiPTHR10488. PTHR10488. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiGATM_BOVIN
AccessioniPrimary (citable) accession number: Q2HJ74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: March 21, 2006
Last modified: July 6, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.